Intracellular Localization of Enzymes in Spleen

Abstract

Some properties of rat spleen ribonuclease have been studied, and the intracellular distribution of the enzyme and ribonucleic acid have been presented. Spleen ribonuclease exhibits maximal activity at pH 5.8, and although there is some evidence for the presence of an enzyme with an optimum at pH 7.0, it is not conclusive. The enzyme is concentrated primarily in the mitochondrial fraction, but significant quantities occur in the supernatant fluid. The latter contains ribonuclease inhibitor similar to that found in liver. The effects of whole body x-irradiation, magnesium ion, substrate concentration, type of buffer, presence of p-chloromercuriphenylsulfonic acid, deoxycholate, and Triton X-100 on ribonuclease activity are examined.

Application of gene specific mRNA level determinations in individual cells using flow cytometry-based PrimeFlow™ in immunotoxicology

“…We have also found that there is a need for optimization based on cell type and tissue of origin. Specifically, high constitutive RNase activity in tissues such as the spleen (Eichel and Roth, 1962), pancreas (Dickman et al , 1960), and lungs (Aho et al , 1983) makes isolation of intact mRNA a challenge. Likewise, we discovered that the elevated levels of B cell RNase activity can be mitigated by simply increasing the concentration of RNase inhibitor.…”

Distribution of Enzymes Between Subcellular Fractions in Animal Tissues

“…The distribution of acid ribonuclease has been investigated in rat liver (10,12,22,96,99,100,318,331,397), including regenerating and precancerous liver (lo), in mouse liver f363), rat kidney (400,401), rat spleen (26,124,334), moure, rabbit, and chicken skeletal muscle (454a), rat brain (23), pig anterior pituitary (229), calf thymus (53), rat mammary gland (156), rat adipose tissue (123), chick embryo Mullerian ducts (42), rabbit leucocytes (76), rat azodye hepatoma, and Novikoff hepatoma (10).…”