Intracellular Localization of the Proteins Encoded by Some Type II Toxin-Antitoxin Systems in Escherichia coli

Abstract

Bacterial toxin-antitoxin (TA) systems, which were discovered in 1985, have since been studied extensively. These studies have focused particularly on the distribution of these bacterial TA systems on either plasmids or on bacterial chromosomes, their functionality, their targets, their relation to virulence, and their mechanisms of action.

Loss of Flagella-Related Genes Enables a Nonflagellated, Fungal-Predating Bacterium To Strengthen the Synthesis of an Antifungal Weapon

“…The protein localization and expression assay was conducted as previously described ( 83 ). To determine protein localization and expression of MotA, MotB, FliG, FliD, FliE, and FlgL in L. enzymogenes OH11.…”

Commentary on “Type II Toxin-antitoxin Systems Have a Peculiar Localization in Escherichia Coli Cells

“…Here we investigated the intracellular localization of the protein products of the following type II TA modules: mazEF, chpBIK, mqsRA and rnlAB [19]. We followed the localization of these proteins by fusing them with the fluorescent protein mCherry.…”