Intracellular mechanics: connecting rheology and mechanotransduction

Mathieu, Samuel; Manneville, Jean‐Baptiste

doi:10.1016/j.ceb.2018.08.007

Cited by 56 publications

(49 citation statements)

References 81 publications

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“…The mapping of cellular mechanics has been achieved using multiple modalities including optical and magnetic tweezer based active rheology, passive rheology, atomic force microscopy and Brillouin microscopy [17,30,31]. Measurements of single cells in suspension can be performed using an optical stretcher and the high throughput, RT-DC system [32].…”

Section: Discussionmentioning

confidence: 99%

“…This mechano-phenotype regulates the kinetics and diversity of enzymatic processes which in turn drive cell fate [13][14][15]. Subcellular components such as the cytoskeleton, nucleus and a copious distribution of cytoplasmic proteins, enzymes and solutes drive the viscoelastic behavior of single cells [13][14][15][16][17]. Thus, defining these mechanical states may offer additional insight in what may drive the interchange between sub types in GBM.…”

Section: Introductionmentioning

confidence: 99%

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Patient-derived glioblastoma cells (GBM) exhibit distinct biomechanical profiles associated with altered activity in the cytoskeleton regulatory pathway

Foss

Zanoni

et al. 2020

Preprint

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Glioblastoma multiforme (GBM) is the most commonly diagnosed brain cancer in adults, characterized by rapid proliferation and aggressive invasion into the stroma. Advances in our understanding of the molecular subtypes of GBM have provided attractive druggable targets. However, the high degree of heterogeneity both among patients and within individual tumors has proven a significant challenge for the development of effective therapies. We hypothesized that this heterogeneity is also represented in the mechanical phenotypes of GBM, as the physical properties of tumor tissue strongly influence elements of tumor progression including cell cycle regulation, migration, and therapeutic resistance. To assess these phenotypes, we employed optical trap-based active microrheology to determine the viscoelastic properties of patient-derived GBM cells in 3D hydrogels mimicking the brain ECM. We found that each GBM cell line had a distinct rheological profile as a function of treatment status, and cell lines could be further characterized by strong power law dependence describing intracellular viscoelastic behavior. Single-cell phenotyping according to power law dependence was able to identify subpopulations of cells within the treatment-resistant line. Finally, proteomic analysis indicated that altered mechanical profiles were associated with differential cytoskeletal regulation, particularly in actin- and myosin-binding pathways. This work suggests that evaluating mechanical properties may serve as a valuable strategy for the further stratification of these tumors, and encourages the investigation of cytoskeleton regulation as a potential therapeutic target for GBM.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Patient-derived glioblastoma cells (GBM) exhibit distinct biomechanical profiles associated with altered activity in the cytoskeleton regulatory pathway

Foss

Zanoni

et al. 2020

Preprint

View full text Add to dashboard Cite

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“…The third one combines both, which may be used to explain how a cell senses and responds to a variety of forces with different spatial and temporal scales. 14…”

Section: Force Transmission By Insoluble Cytoskeleton and Biochemicalmentioning

confidence: 99%

Mechanotransduction in Liver Diseases

Kang

2019

Semin Liver Dis

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Chronic liver diseases, such as fibrosis and cancer, lead to a rigid or stiff liver because of perpetual activation of hepatic stellate cells or portal fibroblasts into matrix-producing myofibroblasts. Mechanical forces, as determined by the mechanical properties of extracellular matrix or pressure of circulating blood flow/shear stress, are sensed by mechanoreceptors at the plasma membrane and transmitted into a cell to impact cell function. This process is termed as mechanotransduction. This review includes basic knowledge regarding how external forces are sensed, amplified, and transmitted into the interior of a cell as far as the nucleus to regulate gene transcription and generate biological responses. It also reviews literatures to highlight the mechanisms by which mechanical forces in a normal or diseased liver influence the phenotype of hepatocytes, hepatic stellate cells, portal fibroblasts, and sinusoidal endothelial cells, and these cells in turn participate in the initiation and progression of liver diseases.

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“…The nervous system is the most complicated system in the body affecting the sensory and motor functions, when the system is damaged. Injuries of the central nervous system (CNS), i.e., brain and spinal cord, lead usually to permanent disability due to severe limitations for spontaneous regeneration of the CNS [1][2][3][4][5][6][7][8][9], leading to considerable socio-economic problems. For instance, 577 cases of traumatic brain injuries (TBI) per 100,000 people per year occurred in the U.S. alone, while, in Europe, the number of patients with diagnosed TBI was estimated at 262 per 100,000 [10].…”

Section: Introductionmentioning

confidence: 99%

Piezoelectric Scaffolds as Smart Materials for Neural Tissue Engineering

2020

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Injury to the central or peripheral nervous systems leads to the loss of cognitive and/or sensorimotor capabilities, which still lacks an effective treatment. Tissue engineering in the post-injury brain represents a promising option for cellular replacement and rescue, providing a cell scaffold for either transplanted or resident cells. Tissue engineering relies on scaffolds for supporting cell differentiation and growth with recent emphasis on stimuli responsive scaffolds, sometimes called smart scaffolds. One of the representatives of this material group is piezoelectric scaffolds, being able to generate electrical charges under mechanical stimulation, which creates a real prospect for using such scaffolds in non-invasive therapy of neural tissue. This paper summarizes the recent knowledge on piezoelectric materials used for tissue engineering, especially neural tissue engineering. The most used materials for tissue engineering strategies are reported together with the main achievements, challenges, and future needs for research and actual therapies. This review provides thus a compilation of the most relevant results and strategies and serves as a starting point for novel research pathways in the most relevant and challenging open questions.

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Intracellular mechanics: connecting rheology and mechanotransduction

Cited by 56 publications

References 81 publications

Patient-derived glioblastoma cells (GBM) exhibit distinct biomechanical profiles associated with altered activity in the cytoskeleton regulatory pathway

Patient-derived glioblastoma cells (GBM) exhibit distinct biomechanical profiles associated with altered activity in the cytoskeleton regulatory pathway

Mechanotransduction in Liver Diseases

Piezoelectric Scaffolds as Smart Materials for Neural Tissue Engineering

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