2015
DOI: 10.1080/10826076.2015.1020164
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Intracellular Metabolomic Approach for Evaluating Antioxidant Capacity and Its Application

Abstract: Metabolomics is frequently used in the analysis of the dynamic changes within small molecules in response to the disturbance in an organism. It has been extensively used in various fields involving human diseases. To better evaluate the antioxidant capacity, cells subjected to oxidative stress were cultured, and the intracellular metabolites were prepared using HSDE (high-speed shear dispersing emulsifier)-assisted extraction. The metabolites were then analyzed under optimized chromatographic conditions. Parti… Show more

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Cited by 2 publications
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“…For example, conventional extraction of anthocyanins is typically conducted at temperatures ranging from 20 to 50°C (Jackman et al, 1987), and temperatures higher than 70°C cause rapid anthocyanin degradation (Havlikova and Mikova, 1985). Liu et al (2015) confirmed that HSDE-assisted extraction could promote the release of intracellular metabolites, and the production was clearly enhanced when the shearing time increased from 0 to 3 min.…”
Section: Discussionmentioning
confidence: 79%
“…For example, conventional extraction of anthocyanins is typically conducted at temperatures ranging from 20 to 50°C (Jackman et al, 1987), and temperatures higher than 70°C cause rapid anthocyanin degradation (Havlikova and Mikova, 1985). Liu et al (2015) confirmed that HSDE-assisted extraction could promote the release of intracellular metabolites, and the production was clearly enhanced when the shearing time increased from 0 to 3 min.…”
Section: Discussionmentioning
confidence: 79%