2020
DOI: 10.1021/acsnano.9b07930
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Intracellular Nanomaterial Delivery via Spiral Hydroporation

Abstract: In recent nanobiotechnology developments, a wide variety of functional nanomaterials and engineered biomolecules have been created, and these have numerous applications in cell biology. For these nanomaterials to fulfill their promises completely, they must be able to reach their biological targets at the subcellular level and with a high level of specificity. Traditionally, either nanocarrier- or membrane disruption-based method has been used to deliver nanomaterials inside cells; however, these methods are s… Show more

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Cited by 58 publications
(77 citation statements)
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“…This method has the highest delivery efficiency and cell viability of all microfluidic techniques. [104,105] Delivery efficiency in hydroporators is a balance between delivery and viability that can be controlled through the Reynolds number of the fluid flow. Notably, nanomaterials with a variety of sizes ranging up to 2000 kDa have been delivered using a platform based on spiral vortex and vortex breakdown due to the flow at the cross and T-junctions.…”
Section: Flow-through Microfluidic Methodsmentioning
confidence: 99%
See 1 more Smart Citation
“…This method has the highest delivery efficiency and cell viability of all microfluidic techniques. [104,105] Delivery efficiency in hydroporators is a balance between delivery and viability that can be controlled through the Reynolds number of the fluid flow. Notably, nanomaterials with a variety of sizes ranging up to 2000 kDa have been delivered using a platform based on spiral vortex and vortex breakdown due to the flow at the cross and T-junctions.…”
Section: Flow-through Microfluidic Methodsmentioning
confidence: 99%
“…Notably, nanomaterials with a variety of sizes ranging up to 2000 kDa have been delivered using a platform based on spiral vortex and vortex breakdown due to the flow at the cross and T‐junctions. [ 104 ]…”
Section: Throughput and Controlmentioning
confidence: 99%
“…Reproduced with permission. [ 211 ] Copyright 2020, American Chemical Society. C) The mechanism of the microfluidic vortex shedding delivery.…”
Section: Micro‐ and Nanoengineered Intracellular Deliverymentioning
confidence: 99%
“…Considerable delivery efficiency of loading different FITC‐dextran sizes (3–2000 kDa), gold nanoparticles, doxorubicin‐loaded mesoporous nanoparticles, and EGFP mRNA have been achieved using spiral hydroporation. [ 211 ]…”
Section: Micro‐ and Nanoengineered Intracellular Deliverymentioning
confidence: 99%
“…Although some progress has been made to reduce costs by improving viral transduction efficiency 11 , additional tools will be necessary to address all of these challenges. Researchers have explored gene-editing tools such as CRISPR/Cas9 combined with alternative gene delivery methods 12 including electrotransfection 13 , lipofection 14,15 , and physical membrane deformation [16][17][18][19] . Electrotransfection in particular has been demonstrated in several cellular therapy manufacturing workflows, including direct delivery of guide RNA (gRNA) complexed with Cas9 protein in the form of a ribonucleoprotein (RNP) complex for gene disruption 20 , delivery of mRNAs that encode for gene-editing proteins 21 , delivery of multiple plasmids as transposon/transposase systems 22 , and co-electroporation of RNPs with a double-stranded DNA (dsDNA) homology-directed repair (HDR) template for totally non-viral, targeted gene insertion 13 .…”
mentioning
confidence: 99%