Intracellular pH and Metabolic Activity of Long-Chain Dicarboxylic Acid-Producing Yeast Candida tropicalis

LIU, SHUCHEN; LI, CHUN; Xie, Langyi; Zhu-an, Cao

doi:10.1263/jbb.96.349

Cited by 4 publications

(6 citation statements)

References 16 publications

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“…Yeast CYP monooxygenase as well as CYP reductase are inducible by hydrocarbons and fatty acids but induction is also dependent on the growth phase, on the inner pH of the cell as well as on oxygen conditions [5,10,25,31,[39][40][41][42].…”

Section: Oxidation Mechanismsmentioning

confidence: 99%

“…After complete metabolization of the carbon source the pH is adjusted to pH 8. The hydrophobic substrate, alkane or fatty acid, is added continuously during this phase together with little amounts of glucose, in order to maintain basal metabolism [80,106,111,112]. It is important to find the balance between limited DCA production at low pH and negative effects on cell viability at high pH values.…”

Section: Bioprocess Engineeringmentioning

confidence: 99%

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Biotechnological synthesis of long‐chain dicarboxylic acids as building blocks for polymers

Huf

Krügener

Hirth

et al. 2011

Eur. J. Lipid Sci. Technol.

121

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An important field in sustainable industrial chemistry is the development of new applications for fats and oils. One of the promising applications is the use of fatty acid derivatives, e.g. dicarboxylic acid (DCA), as polymer building blocks. In contrast to conventional plastics, bioplastics are polymers derived from renewable biomass sources. In addition to their contribution to the conservation of fossil resources and reduction in CO2 emissions by waste incineration, many bioplastics are biodegradable. The majority of industrial DCA production for polyamide (PA) and polyester (PE) synthesis is still done via chemical synthesis. While short-chain DCA can be synthesized in high yields, costs of long-chain DCA production rise significantly due to the generation of various by-products and are connected mostly to a costly purification. Thus biotechnology provides novel biochemical approaches for long-chain DCA synthesis that can provide an eco-efficient process alternative . In the present article, strategies for the development of high-level production strains for long-chain DCA are illustrated. Basic strategies for strain development, in order to achieve an effective enrichment of DCA, require the knowledge of the respective biochemical pathways. These are discussed in detail. Furthermore an overview of fermentation strategies and characteristics of corresponding polymers is given

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Section: Oxidation Mechanismsmentioning

confidence: 99%

Section: Bioprocess Engineeringmentioning

confidence: 99%

Biotechnological synthesis of long‐chain dicarboxylic acids as building blocks for polymers

Huf

Krügener

Hirth

et al. 2011

Eur. J. Lipid Sci. Technol.

121

View full text Add to dashboard Cite

show abstract

“…For an industrial production of DC, bioprocess engineering was carried out to enhance the production of DC by optimizing parameters, such as medium composition , pH , oxygen availability . Scheller et al.…”

Section: Introductionmentioning

confidence: 99%

“…For an industrial production of DC, bioprocess engineering was carried out to enhance the production of DC by optimizing parameters, such as medium composition [7,8], pH [9,10], oxygen availability [11,12]. Scheller et al [11] reported that oxygenation cascaded in conversion of n-alkanes to α, ω-dioic acids catalyzed by cytochrome P450, and Ji et al [13] also indicated that the oxygen supply was of crucial importance for the oxygenation.…”

mentioning

confidence: 99%

Role of oxygen supply in α, ω‐dodecanedioic acid biosynthesis from n‐dodecane by Candida viswanathii ipe‐1: Effect of stirring speed and aeration

Cao

Wang

Luo

et al. 2017

Engineering in Life Sciences

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α, ω‐Dodecanedioic acid (DC12) usually serves as a monomer of polyamides or some special nylons. During the biosynthesis, oxygenation cascaded in conversion of hydrophobic n‐dodecane to DC12, while the oxidation of n‐dodecane took place in the intracellular space. Therefore, it was important to investigate the role of oxygen supply on the cell growth and DC12 biosynthesis. It was found that stirring speed and aeration influenced the dissolved oxygen (DO) concentration which in turn affected cell growth as well as DC12 biosynthesis. However, the effect of culture redox potential (Orp) level on DC12 biosynthesis was more significant than that of DO level. For DC12 biosynthesis, the first step was to form the emulsion droplets through the interaction of n‐dodecane and the cell. When the stirring speed was enhanced, slits in the surface layer of the emulsion droplets would be increased. Thus, the substances transportation by water through the slits would be intensified, leading to an enhanced DC12 production. Compared with the batch culture at a lower stirring speed (400 rpm) without culture redox potential (Orp) control, the DC12 concentration was increased by 5 times up to 201.3 g/L with Orp controlled above 0 mV at a higher stirring speed (800 rpm).

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“…C. tropicalis is one of the main yeast species with high abilities of degrading hydrocarbons represented by nalkanes with long carbon chain (C 10 -C 18 ), benzene and phenolic derivates, through monoterminal oxidation, respectively, the 3-oxoadipate pathway, being also able to assimilate by ω-oxidation, the fatty acids from vegetable oil wastes [3][4][5][6]. Long chain fatty acids and α,ω-dicarboxilic fatty acids resulted from this metabolism are further used in industry for obtaining cosmetics, lubricants, adhesives or plastics [7].…”

mentioning

confidence: 99%

Studies on the Correlation Between Biosurfactant and Lipid Synthesis in Candida tropicalis CMGB114 Using Hydrocarbons and Vegetable Oil Wastes

Csutak¹,

Corbu²,

Vassu³

2017

Rev. Chim.

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During past decades there is an augmented interest regarding the possibility of biosurfactant and lipid production using microorganisms able to assimilate various polluting substrates represented by hydrocarbons and industrial or household wastes such as glycerol and fried vegetable oil. The yeast strain Candida tropicalis CMGB114 produced hhigh rates of biosurfactants when grown on yeast extract and peptone supplemented with 1% petroleum, fried sunflower oil or n-hexadecane, respectively, on yeast extract and NH4NO3 with 40 g/L glycerol. Proliferating lipid bodies were observed in the cells from all the tested culture media, indicating an active lipogenesis and a correlation between biosurfactant and lipid synthesis. Future research will focus on developing strategies for practical applications of C. tropicalis CMGB114 in bioremediation and biofuel production.

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Intracellular pH and Metabolic Activity of Long-Chain Dicarboxylic Acid-Producing Yeast Candida tropicalis

Cited by 4 publications

References 16 publications

Biotechnological synthesis of long‐chain dicarboxylic acids as building blocks for polymers

Biotechnological synthesis of long‐chain dicarboxylic acids as building blocks for polymers

Role of oxygen supply in α, ω‐dodecanedioic acid biosynthesis from n‐dodecane by Candida viswanathii ipe‐1: Effect of stirring speed and aeration

Studies on the Correlation Between Biosurfactant and Lipid Synthesis in Candida tropicalis CMGB114 Using Hydrocarbons and Vegetable Oil Wastes

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