Intracellular pH measurement during high-pressure CO2 pasteurization evaluated by cell fluorescent staining

Spilimbergo, Sara; Quaranta, A.; Garcia‐Gonzalez, Linsey; Contrini, C.; Cinquemani, Claudio; Ginneken, Luc Van

doi:10.1016/j.supflu.2010.03.004

Cited by 21 publications

(5 citation statements)

References 24 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…One treatment that has attracted significant interest is supercritical carbon dioxide (scCO 2 ) at pressures of 2–20 mega Pascals (mPa) and temperatures from 25 to 50°C (Spilimbergo et al . , ; White et al . ; Park et al .…”

Section: Introductionmentioning

confidence: 99%

“…Consequently, there is significant interest in ways to inactivate spores, in particular using procedures that do not damage sensitive drugs, biomaterials or medical devices. One treatment that has attracted significant interest is supercritical carbon dioxide (scCO 2 ) at pressures of 2-20 mega Pascals (mPa) and temperatures from 25 to 50°C (Spilimbergo et al 2003(Spilimbergo et al , 2010White et al 2006;Park et al 2013;Tamburini et al 2014a,b;Bernhardt et al 2015), conditions that alone are not especially damaging to drugs or devices. However, while such conditions can be efficient at inactivating growing bacteria of a variety of species, generally higher temperatures or pressures are needed to efficiently inactivate spores (Ishikawa et al 1997;Rao et al 2015).…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Mechanism of Bacillus subtilis spore inactivation by and resistance to supercritical CO₂ plus peracetic acid

et al. 2015

View full text Add to dashboard Cite

Aims Determine how supercritical CO2 (scCO2) plus peracetic acid (PAA) inactivates Bacillus subtilis spores, factors important in spore resistance to scCO2-PAA, and if spores inactivated by scCO2-PAA are truly dead. Methods and Results Spores of wild-type B. subtilis and isogenic mutants lacking spore protective proteins were treated with scCO2-PAA in liquid or dry at 35°C. Wild-type wet spores (aqueous suspension) were more susceptible than dry spores. Treated spores were examined for viability (and were truly dead), dipicolinic acid (DPA), mutations, permeability to nucleic acid stains, germination under different conditions, energy metabolism and outgrowth. ScCO2-PAA-inactivated spores retained DPA, and survivors had no notable DNA damage. However, DPA was released from inactivated spores at a normally innocuous temperature (85°C), and colony formation from treated spores was salt sensitive. The inactivated spores germinated but did not outgrow, and these germinated spores had altered plasma membrane permeability and defective energy metabolism. Wet or dry coat-defective spores had increased scCO2-PAA sensitivity, and dry spores but not wet spores lacking DNA protective proteins were more scCO2-PAA sensitive. Conclusions These findings suggest that scCO2-PAA inactivates spores by damaging spores’ inner membrane. The spore coat provided scCO2-PAA resistance for both wet and dry spores. DNA protective proteins provided scCO2-PAA resistance only for dry spores. Significance and Impact of Study These results provide information on mechanisms of spore inactivation of and resistance to scCO2-PAA, an agent with increasing use in sterilization applications.

show abstract

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Mechanism of Bacillus subtilis spore inactivation by and resistance to supercritical CO₂ plus peracetic acid

et al. 2015

View full text Add to dashboard Cite

show abstract

“…Firstly, high pressure in HPCD was applied in a way similar to HHP, though treatment conditions were more moderate. Secondly, HPCD caused a pH decrease in the suspending medium and the interior of microbial cells due to carbonic acid formation on dissolution of HPDC, as shown in previous studies (Spilimbergo et al, 2005(Spilimbergo et al, , 2010. The pH decrease, both externally and internally of microbial cells, would induce DNA denaturation as DNA is sensitive to extreme acidic environments.…”

Section: Introductionmentioning

confidence: 51%

Effects of high-pressure carbon dioxide on proteins and DNA in Escherichia coli

Liao

Zhang

et al. 2011

Microbiology

View full text Add to dashboard Cite

Protein changes in Escherichia coli, when subjected to high-pressure carbon dioxide (HPCD) at 10 MPa and 376C for 5-75 min, were assessed using the Bradford method, 2D electrophoresis (2-DE) and liquid chromatography-electrospray ionization-MS-MS (LC-ESI-MS-MS). The changes in DNA in E. coli under the same conditions were also investigated by using flow cytometry with propidium iodide and acridine orange, agarose gel electrophoresis (AGE) and the comet assay. The results showed that HPCD induced leakage loss of the proteins and DNA of E. coli as a function of treatment time. With regard to the protein changes, 182 proteins in the 2-DE profile were not found in the HPCD-treated E. coli. Among 20 selected protein spots exhibiting significant changes in intensity, 18 protein spots were identified as 15 known proteins and two as hypothetical proteins. These proteins were involved in cell composition, energy metabolism pathways, nucleic acid metabolism, global stress regulation and general metabolism. The DNA denaturation of E. coli induced by HPCD was demonstrated in this study for the first time to our knowledge, and the denaturation was enhanced by increasing treatment time. However, HPCD did not cause DNA degradation, as suggested by both AGE analysis and the comet assay.

show abstract

“…We hypothesize that, beside the recently proven inactivation effect of pH drop [42], CO 2 could fluidize the cell wall (and maybe also the spore wall to a smaller extent), and thus enable a sterilizing agent to enter the cell/core. As solvent power varies with pressure [4] so does partition coefficient, which is key in inactivation of vegetative germs [22,23].…”

Section: Resultsmentioning

confidence: 99%

Human pathogens, nosocomial infections, heat-sensitive textile implants, and an innovative approach to deal with them

Cinquemani

2010

J Ind Microbiol Biotechnol

View full text Add to dashboard Cite

Intracellular pH measurement during high-pressure CO2 pasteurization evaluated by cell fluorescent staining

Cited by 21 publications

References 24 publications

Mechanism of Bacillus subtilis spore inactivation by and resistance to supercritical CO₂ plus peracetic acid

Mechanism of Bacillus subtilis spore inactivation by and resistance to supercritical CO₂ plus peracetic acid

Effects of high-pressure carbon dioxide on proteins and DNA in Escherichia coli

Human pathogens, nosocomial infections, heat-sensitive textile implants, and an innovative approach to deal with them

Contact Info

Product

Resources

About

Intracellular pH measurement during high-pressure CO2 pasteurization evaluated by cell fluorescent staining

Cited by 21 publications

References 24 publications

Mechanism of Bacillus subtilis spore inactivation by and resistance to supercritical CO2 plus peracetic acid

Mechanism of Bacillus subtilis spore inactivation by and resistance to supercritical CO2 plus peracetic acid

Effects of high-pressure carbon dioxide on proteins and DNA in Escherichia coli

Human pathogens, nosocomial infections, heat-sensitive textile implants, and an innovative approach to deal with them

Contact Info

Product

Resources

About

Mechanism of Bacillus subtilis spore inactivation by and resistance to supercritical CO₂ plus peracetic acid

Mechanism of Bacillus subtilis spore inactivation by and resistance to supercritical CO₂ plus peracetic acid