Intracellular reaction rates, enzyme activities and biomass yields in Methylomonas L3: growth rate and substrate composition effects

Bussineau, C. M.; Papoutsakis, Eleftherios T.

doi:10.1007/bf00250319

Cited by 12 publications

(2 citation statements)

References 16 publications

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“…The reaction stoichiometry for PHB production from acetate was derived from the measurements and the biomass yield on PHB was obtained from (Van Aalst-Van Leeuwen et al, 1994). For methanol, due to lack of experimental data, the stoichiometric growth yield from (Bussineau and Papoutsakis, 1986) was used ( Table 3). The operational conditions including the variables tested are reported in Table 4.…”

Section: Kinetic Modelmentioning

confidence: 99%

Survival of the fastest: Selective removal of the side population for enhanced PHA production in a mixed substrate enrichment

Korkakaki

Loosdrecht

Kleerebezem

2016

Bioresource Technology

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Section: Kinetic Modelmentioning

confidence: 99%

Survival of the fastest: Selective removal of the side population for enhanced PHA production in a mixed substrate enrichment

Korkakaki

Loosdrecht

Kleerebezem

2016

Bioresource Technology

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“…Methane monooxygenase from several species of methanotrophs has been shown to insert an oxygen atom into a wide range of substrates (Anthony, 1975;Anthony, 1982;Bussineau and Papoutsakis, 1988;Bussineau and Papoutsakis, 1986;Bussineau et al, 1987). These include carbon monoxide, ammonia, n-alkanes, n-alkenes, dimethyl and diethyl ether, alicyclic and aromatic hydrocarbons.…”

Section: Introductionmentioning

confidence: 99%

Fossil Energy Biotechnology

1993

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Portions of this document may be illegible in electronic image products.Images are produced from the best avaiiable original document. EXPERT PANELThe following panel of distinguished scientists evaluated the research needs for fossil energy biotechnology applicable to the programs of the Department of Energy. This project was conducted under ,contract to Consultec Scientific, Inc., Knoxville, TN from the US.

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Growth dynamics of a methylotroph (Methylomonas L3) in continuous cultures. II. Growth inhibition and comparison against an unstructured model

Chu

Papoutsakis

1987

Biotech & Bioengineering

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High methanol concentrations have a negative effect on the growth rate and the biomass yield of growth transients induced by methanol pulses in continuous cultures of Methylomonas L3. The physiological basis of this effect is investigated by measuring the effect of the methanol pulse on the cell energy charge (EC) and ATP, ADP, and AMP concentrations, and by comparing the results of the pulse transients against an unstructured model. The methanol pulse is shown to lead to increased values of the cell EC and ATP concentration, and thus, inhibition and reduced availability of biosynthetic energy are excluded as causes of inhibition. When the biomass and methanol profiles of the transient experiments are compared in phase-plane diagrams against computer simulations based on the model, satisfactory agreement between experimental data and model predictions is found in single-substrate, high-dilution-rate experiments. Conversely, poor agreement between experimental data and simulation results indicates a more severe growth inhibition than the model predicts at low dilution rates and a less severe one in mixed-substrate experiments. Based on these findings and other relevant physiological information, we propose that the large variations in the negative effect of methanol on growth result from the fact that cells accumulate methanol to widely different concentrations depending on their physiological state. In their effort to detoxify from the high intracellular methanol and formaldehyde concentrations, cells oxidize considerably more methanol than they can incorporate into biomass. This leads to a useless ATP surplus, which the cells must hydrolyze without doing any useful biosynthetic work, and this results in lower biomass yields.

show abstract

Intracellular reaction rates, enzyme activities and biomass yields in Methylomonas L3: growth rate and substrate composition effects

Cited by 12 publications

References 16 publications

Survival of the fastest: Selective removal of the side population for enhanced PHA production in a mixed substrate enrichment

Survival of the fastest: Selective removal of the side population for enhanced PHA production in a mixed substrate enrichment

Fossil Energy Biotechnology

Growth dynamics of a methylotroph (Methylomonas L3) in continuous cultures. II. Growth inhibition and comparison against an unstructured model

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