Intracellular recordings from neurobiotin-labeled cells in brain slices of the rat medial nucleus of the trapezoid body

Banks, Matthew I.; Ph, Smith

doi:10.1523/jneurosci.12-07-02819.1992

Cited by 243 publications

(241 citation statements)

References 46 publications

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“…Both GABA and glycine caused a decrease in the input resistance of MNTB neurons, resulting in subthreshold EPSPs and the loss of action potential firing (Wu and Kelly 1995). In addition, long-latency IPSPs evoked by stimulation of the trapezoid body could be completely blocked by strychnine (Banks and Smith 1992).…”

Section: Failure Of Spike Transmissionmentioning

confidence: 99%

“…This inhibitory influence could be mediated through the GABAergic and glycinergic inputs to MNTB somata that have been described in immunocytochemical and electronmicroscopical studies (Roberts and Ribak 1987;Adams and Mugnaini 1990;Cant 1991). Further evidence for postsynaptic inhibition and the functional role of these transmitters comes from slice electrophysiological studies (Banks and Smith 1992;Wu and Kelly 1995) and GABA (Wu and Kelly 1995). Both GABA and glycine caused a decrease in the input resistance of MNTB neurons, resulting in subthreshold EPSPs and the loss of action potential firing (Wu and Kelly 1995).…”

Section: Failure Of Spike Transmissionmentioning

confidence: 99%

“…The glycinergic terminals arise, in part, from recurrent projections of MNTB neurons Smith et al 1998). To date, the physiological effect of inhibitory neurotransmitters and of inhibitory input to the MNTB has been studied exclusively using slice preparations (Banks and Smith 1992;Forsythe and Barnes-Davies 1993b;Wu and Kelly 1995;Turecek and Trussell 2001). Electrical stimulation of the trapezoid body in rat brain slices gives rise to IPSPs in MNTB neurons, which can be blocked by strychnine (Banks and Smith 1992; Forsythe and Barnes-Davies 1993b).…”

Section: Introductionmentioning

confidence: 99%

“…To date, the physiological effect of inhibitory neurotransmitters and of inhibitory input to the MNTB has been studied exclusively using slice preparations (Banks and Smith 1992;Forsythe and Barnes-Davies 1993b;Wu and Kelly 1995;Turecek and Trussell 2001). Electrical stimulation of the trapezoid body in rat brain slices gives rise to IPSPs in MNTB neurons, which can be blocked by strychnine (Banks and Smith 1992; Forsythe and Barnes-Davies 1993b). In addition, the membrane resistance of MNTB cells in the mouse is lowered by application of GABA and glycine (Wu and Kelly 1995).…”

Section: Introductionmentioning

confidence: 99%

“…The innervation of MNTB neurons by extrinsic and intrinsic inhibitory afferents, the effect of inhibitory neurotransmitters, and the fact that the MNTB projects to several other brainstem auditory nuclei in addition to the LSO (Kuwabara and Zook 1991;Schofield and Cant 1991;Banks and Smith 1992;Sommer et al 1993;Schofield 1994;Smith et al 1998) suggest that the MNTB does not serve only as a sign-converting relay to the LSO. To test this hypothesis, the excitatory afferent input to the MNTB neuron and the firing of the MNTB neuron itself were simultaneously monitored with the same electrode during in vivo extracellular recordings in the gerbil.…”

Section: Introductionmentioning

confidence: 99%

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The Medial Nucleus of the Trapezoid Body in the Gerbil Is More Than a Relay: Comparison of Pre- and Postsynaptic Activity

Kopp-Scheinpflug

Lippe

Dörrscheidt

et al. 2003

JARO - Journal of the Association for Research in Otolaryngolog

114

158

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The medial nucleus of the trapezoid body (MNTB) plays an important role in the processing of interaural intensity differences, a feature that is critical for the localization of sound sources. It is generally believed that the MNTB functions primarily as a passive relay in converting excitatory input originating from the contralateral cochlear nucleus (CN) into an inhibitory input to the ipsilateral lateral superior olive. However, studies showing that the MNTB itself is also the target of inhibitory input suggest that the MNTB may serve more than a sign-converting function. To examine the fidelity of signal transmission at the CN-MNTB synapse, presynaptic calyceal potentials (''prepotentials''), reflecting the excitatory input to the MNTB neuron, and postsynaptic action potentials were simultaneously monitored with the same electrode during in vivo extracellular recordings from the gerbil's MNTB. Presynaptic activity differed from postsynaptic activity in several respects: (1) Spontaneous and sound-evoked discharge rates were greater presynaptically than postsynaptically. (2) Frequency tuning was sharper postsynaptically than presynaptically. (3) Calyceal terminals and MNTB neurons both showed phasic-tonic response patterns to tonal stimulation, but the duration of the onset response and the level of the tonic component were reduced postsynaptically. (4) Phase-locking to sound frequencies up to 1 kHz was greater postsynaptically than presynaptically. (5) The rate-intensity characteristics of pre-and postsynaptic activities differed significantly from each other in half of the MNTB neurons. To test the hypothesis that acoustically evoked inhibition of MNTB neurons contributed to the relatively lower levels of postsynaptic discharge, two-tone stimulation was applied, wherein the response to one tone-burst, set at the neuron's characteristic frequency, can be reduced by addition of a second ''inhibitory'' tone. The inhibitory tone caused a much larger reduction in post-than in presynaptic activity, indicating an acoustically evoked inhibitory influence directly on MNTB units. These findings show that transmission at the CN-MNTB synapse does not occur in a fixed one-to-one manner and that the response of MNTB neurons reflects the integration of their excitatory and inhibitory inputs.

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Section: Failure Of Spike Transmissionmentioning

confidence: 99%

Section: Failure Of Spike Transmissionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 3 more Smart Citations

The Medial Nucleus of the Trapezoid Body in the Gerbil Is More Than a Relay: Comparison of Pre- and Postsynaptic Activity

Kopp-Scheinpflug

Lippe

Dörrscheidt

et al. 2003

JARO - Journal of the Association for Research in Otolaryngolog

114

158

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Organization of ventrolateral periolivary cells of the cat superior olive as revealed by PEP-19 immunocytochemistry and Nissl stain

Spirou

Berrebi

1996

J. Comp. Neurol.

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Ventrolateral periolivary cell groups, through their descending projections to the cochlear nucleus (CN) and local projections to principal nuclei of the superior olive, may participate in brainstem mechanisms mediating such tasks as signal detection in noisy environments and sound localization. Understanding the function of these cell groups can be improved by increased knowledge of the organization of their synaptic inputs in relation to their cellular characteristics. Immunocytochemistry for PEP-19 (a putative calcium binding protein) reveals four patterns of immunolabeling within the ventrolateral periolivary region. Three of the patterns, which have distinct fiber and punctate labeling characteristics, help to define three subdivisions of the lateral nucleus of the trapezoid body (LNTB). The fourth pattern defines two other nuclei, the anterolateral periolivary nucleus (rostral) and the posterior periolivary nucleus (caudal), which display many immunoreactive cell bodies but little fiber and punctate labeling. One of the subdivisions of the LNTB contains large PEP-19 immunolabeled puncta arranged in pericellular nests. Analysis of Nissl-stained sections reveals a neuronal population that resembles globular cells of the ventral cochlear nucleus (VCN) and which colocalizes with pericellular nests of large immunolabeled puncta. Cell counts reveal that roughly 10,000 neurons constitute the cat ventrolateral periolivary region, 9,000 of which are found in the LNTB. Three-dimensional reconstructions of auditory brainstem nuclei clarify the complex spatial relationships among these structures.

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Temporary sensory deprivation changes calcium-binding proteins levels in the auditory brainstem

et al. 1997

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Auditory brainstem neurons probably depend on afferent input to maintain calcium homeostasis within a narrow range. These neurons are endowed with high concentrations of the calcium-binding proteins parvalbumin, calretinin, and calbindin D28k that are presumed to buffer cytosolic calcium transients. To determine the effects of functional deafferentation on these proteins in the auditory brainstem of adult guinea pigs, we have manipulated the sensory input with an intracochlear perfusion of the glutamate agonist alpha-amino-3-hydroxy-5-methyl-4-isoxazole propionic acid (AMPA), which is known to transiently disconnect inner hair cells and primary auditory dendrites. Semiquantitative measures of immunostaining intensities showed rapid and reversible changes in calcium-binding protein levels. By 24 hours after AMPA treatment, calretinin immunostaining was reduced in deafferented neurons of the cochlear nuclei and their axons in the superior olivary nuclei. In contrast, calbindin D28k immunoreactivity levels by this time were higher in deafferented neurons of the medial nucleus of the trapezoid body and their axons in the lateral superior olivary nucleus (LSO). Parvalbumin immunostaining was also generally increased in deafferented neurons, but changes were less evident and more complex. The changes in all three immunoreactivities disappeared with the progressive restoration of afferent input. Normal levels were reestablished by 5 days after AMPA treatment, when afferent activity had almost completely recovered. These results show that calcium-binding protein immunostaining in auditory neurons is functionally responsive to afferent activity. The increased buffering capacity in deafferented neurons as shown by the rises in parvalbumin and calbindin D28k immunostaining may be part of mechanisms promoting neuronal survival after loss of sensory input. This input, on the other hand, may be necessary for maintaining the high calretinin levels normally present in cochlear nucleus neurons.

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Intracellular recordings from neurobiotin-labeled cells in brain slices of the rat medial nucleus of the trapezoid body

Cited by 243 publications

References 46 publications

The Medial Nucleus of the Trapezoid Body in the Gerbil Is More Than a Relay: Comparison of Pre- and Postsynaptic Activity

The Medial Nucleus of the Trapezoid Body in the Gerbil Is More Than a Relay: Comparison of Pre- and Postsynaptic Activity

Organization of ventrolateral periolivary cells of the cat superior olive as revealed by PEP-19 immunocytochemistry and Nissl stain

Temporary sensory deprivation changes calcium-binding proteins levels in the auditory brainstem

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