Intracellular RNAs of the Feline Infectious Peritonitis Coronavirus Strain 79-1146

Groot, Raoul J. de; Haar, Rohini J.; Horzinek, Marian C.; Zeijst, Bernard A.M. van der

doi:10.1099/0022-1317-68-4-995

Cited by 52 publications

(49 citation statements)

References 31 publications

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“…Assuming an RNA leader sequence of 60-70 nucleotides (Spaan et al, 1983;Lai eta/., 1984;Brown eta/., 1984) and a poly(A) tail of about 100 nucleotides we arrive at a predicted length of approximately 1400 nucleotides. Previously, RNA 6 was estimated to be 1600 nucleotides (de Groot et aL, 1987a). By using gels with a better resolution in this MW range, we have now measured a length of about 1450 nucle0tides (not shown).…”

Section: Resultsmentioning

confidence: 60%

“…Differences in the mRNA sets of TGEV Purdue and FIPV 79-1146 indicated the presence of large insertions at the 3' end of the FIPV genome (de Groot et al, 1987a). We have characterized these insertions by sequence analysis of recombinants which had been isolated from a FIPV-specific cDNA library by hybridization with the smallest FIPV mRNA (RNA 6).…”

Section: Discussionmentioning

confidence: 99%

“…In vitro translation of the viral mRNAs (Rottier et al, 1981;Siddell, 1983;Stern and Sefton, 1984;Jacobs et al, 1986;de Groot et aL, 1987a) and the sequence analysis of coronavirus genomes Rasschaert et al, 1987;Skinner and Siddell, 1985;Schmidt et aL, 1987;Luytjes et aL, 1987) have allowed the construction of genomic maps. The relative position of the genes encoding the structural proteins is conserved on the genomes of these viruses.…”

Section: Introductionmentioning

confidence: 99%

“…However, differences in the genomic maps indicated that other transcription units have been lost, gained, or transloTo whom requests for reprints should be addressed. cated as the coronaviruses diverged (de Groot et al, 1987a).…”

Section: Introductionmentioning

confidence: 99%

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Sequence analysis of the 3′ end of the feline coronavirus FIPV 79-1146 genome: Comparison with the genome of porcine coronavirus TGEV reveals large insertions

Groot¹,

Andeweg²,

Horzinek³

et al. 1988

Virology

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The genetic information, carried on mRNA 6 of feline infectious peritonitis virus (FIPV) strain 79-1146, was determined by sequence analysis of cDNA clones derived from the 3' end of the FIPV genome. Two ORFs were found, encoding polypeptides of 11K (ORF-1) and 22K (ORF-2). The FIPV sequence was compared to the 3' end sequence of transmissible gastroenteritis virus (TGEV). ORF-1 has a homologous counterpart (ORF-X3) in the TGEV genome; both ORFs are located at the same position relative to the nucleocapsid gene. However, as a result of an in-frame insertion or deletion, ORF-1 is 69 nucleotides larger than ORF-X3. A similar event has occurred immediately downstream of ORF1 : a 624-nucleotide segment, containing the complete ORF-2, is absent in the TGEV sequence. Most sequence similarity (98.5%) was found in the 3' noncoding sequences. ORF-X3 and ORF-1 are preceded by the sequence AAC-TAAAC, which is assumed to be the transcription-initiation signal in FIPV and TGEV (P. A. Kapke and D. A. Brian (1986) Virology 151,[41][42][43][44][45][46][47][48][49]. By Sl nuclease analysis, the 5' end of FIPV RNA 6 was mapped immediately upstream of this sequence. A 700-nucleotide TGEV-specific RNA was found by cross-hybridization with an FIPV 3' end probe, suggesting that TGEV ORF-X3 is also carried on a separate mRNA. The differences at the 3' ends of the FIPV and TGEV genomes may be the result of RNA recombination events.

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Section: Resultsmentioning

confidence: 60%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Sequence analysis of the 3′ end of the feline coronavirus FIPV 79-1146 genome: Comparison with the genome of porcine coronavirus TGEV reveals large insertions

Groot¹,

Andeweg²,

Horzinek³

et al. 1988

Virology

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“…In a previous report (de Groot et al, 1987a) we have shown that in FIPV-infected cells six virus-specific RNAs are produced with sizes ranging from 1.6 to at least 20 kb. Data on the RNAs and their coding assignment are summarized in Fig.…”

Section: Introductionmentioning

confidence: 93%

cDNA Cloning and Sequence Analysis of the Gene Encoding the Peplomer Protein of Feline Infectious Peritonitis Virus

Groot

Maduro

Lenstra

et al. 1987

Journal of General Virology

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SUMMARYThe peplomer gene of feline infectious peritonitis virus (FIPV) strain 79-1146 was isolated from a genomic cDNA library by differential hybridization with RNA 2 and 3 as probes. From the nucleotide sequence a primary translation product of 1452 residues (Mr 160472) was predicted, containing an N-terminal signal sequence, a C-terminal transmembrane segment and 35 potential N-linked glycosylation.sites. By S1 nuclease analysis the 5' end of the presumptive RNA 2 body was located at about 30 nucleotides upstream from the initiating AUG codon. At approximately the same position a nine nucleotide sequence ACUAAACUU was found, which was also present 37 nucleotides downstream from the open reading frame. Comparison of the sequences of the FIPV, murine hepatitis virus and infectious bronchitis virus peplomer proteins showed about 27% overall homology, with most conservation in the C-terminal half.

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The Genome Organization of the Nidovirales: Similarities and Differences between Arteri-, Toro-, and Coronaviruses

Vries

Horzinek

Rottier

et al. 1997

Seminars in Virology

256

217

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Viruses in the families Arteriviridae and Coronaviridae have enveloped virions which contain nonsegmented, positive-stranded RNA, but the constituent genera differ markedly in genetic complexity and virion structure. Nevertheless, there are striking resemblances among the viruses in the organization and expression of their genomes, and sequence conservation among the polymerase polyproteins strongly suggests that they have a common ancestry. On this basis, the International Committee on Taxonomy of Viruses recently established a new order, Nidovirales, to contain the two families. Here, the common traits and distinguishing features of the Nidovirales are reviewed. r 1997 Academic Press

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Intracellular RNAs of the Feline Infectious Peritonitis Coronavirus Strain 79-1146

Cited by 52 publications

References 31 publications

Sequence analysis of the 3′ end of the feline coronavirus FIPV 79-1146 genome: Comparison with the genome of porcine coronavirus TGEV reveals large insertions

Sequence analysis of the 3′ end of the feline coronavirus FIPV 79-1146 genome: Comparison with the genome of porcine coronavirus TGEV reveals large insertions

cDNA Cloning and Sequence Analysis of the Gene Encoding the Peplomer Protein of Feline Infectious Peritonitis Virus

The Genome Organization of the Nidovirales: Similarities and Differences between Arteri-, Toro-, and Coronaviruses

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