Intracellular ROS scavenging and antioxidant regulation of WL15 from cysteine and glycine-rich protein 2 demonstrated in zebrafish in vivo model

Guru, Ajay; Lite, Christy; Freddy, Allen J.; Issac, Praveen Kumar; Pasupuleti, Mukesh; Saraswathi, Nadar; Arasu, Mariadhas Valan; Al-Dhabi, Naïf Abdullah; Arshad, Aziz; Arockiaraj, Jesu

doi:10.1016/j.dci.2020.103863

Cited by 74 publications

(43 citation statements)

References 57 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Some other findings suggested that both hydrophobic and aromatic amino acids help to increase the antioxidant properties of the peptide (Xiong et al 2013). In accordance with these reports, RM12 consists of hydrophobic and aromatic amino acids including Ile (8%), Leu (8%), Phe (8%), Met (8%), Gly (8%), and Pro (16%); altogether, RM12 contributes antioxidant properties, thus scavenging free radicals (Guru et al 2021).…”

Section: Discussionsupporting

confidence: 75%

“…The acetate group of DCFH-DA was cleaved by the intracellular esterase and formed a cell membrane-impermeable product, H 2 DCF, which undergo intracellular oxidation that leads to a higher yield of the fluorescent product DCF. RM12 peptide has shown significant activity to inhibit the production of fluorescent product DCF in larvae after exposure to ROS inducer, H 2 O 2 (Guru et al 2021). The quantitative analysis of DCFH-DA was carried out to evaluate the intracellular ROS level in the zebrafish embryo lysate, and the fluorescence was determined through a microplate reader at 529 nm.…”

Section: Discussionmentioning

confidence: 99%

“…Hemolytic assay using fish erythrocytes C. striatus blood (2 ml) was collected, centrifuged, and obtained erythrocyte pellets. The hemolytic assay was performed using the pellets along with different concentrations of RM12 peptide (10 μM, 20 μM, 40 μM, 80 μM, 160 μM, and 320 μM) (Guru et al 2021). The erythrocytes were washed and rinsed thrice with 1X PBS.…”

Section: Toxicity Analysis Of Rm12mentioning

confidence: 99%

See 2 more Smart Citations

RM12 similar to substance P from tachykinin of freshwater murrel Channa striatus influence intracellular ROS in vitro fish erythrocytes and developmental toxicity and antioxidant enzymes in vivo zebrafish embryo

Raju

Mukherjee

Sarkar

et al. 2021

Fish Physiol Biochem

Self Cite

View full text Add to dashboard Cite

zebrafish embryo, the intracellular ROS level was estimated by 5 mM H 2 O 2 stress in the zebrafish embryo, and inhibition of apoptosis was evaluated. The antioxidant enzymes were extracted from the H 2 O 2 -stressed zebrafish embryo, and the intracellular ROS was eliminated due to RM12. Collectively, the experiment showed that the substance P from the freshwater murrel C. striatus possessed potent antioxidant properties; thus, it can further be focused to develop it as antioxidant molecule in aquaculture organisms. KeywordsSubstance P • Tachykinin • Channa striatus • Antioxidant mechanism • Intracellular ROS Abbreviations ROS Reactive oxygen species H 2 O 2 Hydrogen peroxide ESR Electron spin resonance DPPH 2,2-Diphenyl-1-picryl-hydrazyl-hydrate ABTS 2,2-Azinobis 3-ethylbenzothiazoline-6-sulfonic acid SOAH Superoxide anion radical scavenging assay HORS Hydroxyl radical scavenging assay DCFH-DA 2′-7′-Dichlorodihydrofluorescein diacetate TEAC Trolox equivalent antioxidant capacity PMS Phenazine methosulphate NADH Nicotinamide adenine dinucleotide NBT Nitro blue tetrazolium

show abstract

Section: Discussionsupporting

confidence: 75%

Section: Discussionmentioning

confidence: 99%

Section: Toxicity Analysis Of Rm12mentioning

confidence: 99%

See 1 more Smart Citation

RM12 similar to substance P from tachykinin of freshwater murrel Channa striatus influence intracellular ROS in vitro fish erythrocytes and developmental toxicity and antioxidant enzymes in vivo zebrafish embryo

Raju

Mukherjee

Sarkar

et al. 2021

Fish Physiol Biochem

Self Cite

View full text Add to dashboard Cite

show abstract

“… Kalaitzoglou et al (2016) , proposed that T1D hampers the bone formation process through negative regulation of osteoblasts and osteocytes, along with hyperactivated osteoclast functions, culminating in bone resorption and delayed fracture healing. The fracture healing occurs in two stages (i) anabolic stage: fractured bone healing and repair of the tissue in skeletal vicinity result in stem cell-based osteovascular regeneration, tissue volume accrual and subsequent callus formation; (ii) catabolic stage: resorption of bony callus tissue results in the metamorphosis of callus into cortical bone through “coupled remodelling”, a process manifested by coupled cycles of osteoblast and osteoclast activities ( Einhorn and Gerstenfeld, 2015 , Guru et al, 2020 ).…”

Section: Introductionmentioning

confidence: 99%

Neurokinin-1-tachykinin receptor agonist promotes diabetic fracture healing in rats with type 1 diabetes via modulation of Wnt/β-catenin signalling axis

Wang

2021

Saudi Journal of Biological Sciences

View full text Add to dashboard Cite

Diabetes mellitus is an ill-famed metabolic disorder with varied repercussions including delayed fracture healing. Wnt/β-catenin axis is known to play a tight pivotal role in the bone healing process. Substance P (SubP) is a neuropeptide with established positive modulatory functions in fracture healing and associated neuronal milieu. In this study, we performed local delivery of recombinant adenovirus of Dickkopf-1 (DKK1) into the fracture site to understand the antagonizing the role of DKK1 against substance P. Rats were segregated into 4 groups: (i) Fractured non-diabetic rats; (ii) Fractured T1D rats; T1D was provoked by using STZ 50 mg/kg for 5 consecutive days; (iii) Fractured T1D + SubP (50 mg/ml/Kg; i.p.; 30 min prior to fracture procedure); (iv) Fractured T1D + SubP + Ad-DKK1. Bone radiographs were taken using a Faxitron X-ray machine and the residual gap size was measured using an electric caliper. Western blotting was also performed to determine the protein expression levels of osteogenic markers (RUNX2, OSTX and OSTC) bone resorption markers (OPG, RANKL and RANK) and also Wnt-signalling markers (β-catenin, LRP5 and GSK-3β). We observed that SubP promoted osteogenesis (as indicated by RUNX2, OSTX and OSTC upregulation) and mitigated the bone resorption (as indicated by optimized OPG/RANKL/RANK axis) via activated Wnt signalling (manifested by upmodulated β-catenin and LRP5, with downmodulated GSK-3β levels. Activation of endogenous SubP or administration of exogenous mimics might counter-protect the fractured bone against the deforming effects of T1D.

show abstract

“…Antioxidant regulation properties of WL15 from glycine-rich protein 2 and cysteine was reported previously ( Sannasimuthu et al, 2020 , Issac et al, 2020 ). Zebra fish model has been widely used to analyze antioxidant properties of natural sources to treat types of cancers ( Guru et al, 2020 ). The present research study aimed to evaluate the role of miRNA-194 and miRNA-192 in NSCLC participants.…”

Section: Introductionmentioning

confidence: 99%

Dysregulation of exosomal miR-192 and miR-194 expression in lung adenocarcinoma patients

Wang

et al. 2021

Saudi Journal of Biological Sciences

View full text Add to dashboard Cite

Non-small cell lung cancer (NSCLC) is the main reason of cancer linked mortality and around 80% of cases diagnosed in advanced stage. Therefore current study designed to evaluate the deregulation of miRNA-194 and miRNA-192 in different body fluid of Non small cell lung cancer participants. Present study recruited newly diagnosed histopathologically confirmed. It was observed that the 40% NSCLC participants showed elevated miR-194 expression and 60% NSCLC participants showed reduced miR-194 expression in serum sample while in Bronchial wash, only 20% NSCLC participants showed elevated miR-194 expression while 80% showed reduced miR-194 expression (p = 0.003). It was found that the 54% NSCLC participants showed elevated miR-192 expression and 55% NSCLC participants showed reduced miR-192 expression in serum sample while In Bronchial wash sample, only 25% NSCLC participants showed high miR-192 expression while 75% showed low miR-192 expression (P = 0.0004). Expression of miR-194 was significantly associated with TNM stages (p < 0.0001, p < 0.0001), distant organ metastases (p < 0.0001, p < 0.0001), pathological grade (p = 0.0009, p = 0.0005) among serum sample and bronchial wash sample. Same observation was found with expression of miR-192 and it was significantly associated with TNM stages (p < 0.0001, p < 0.0001), distant organ metastases (p < 0.0001, p < 0.0001), pathological grade (p = 0.006, p = 0.001) among serum sample and bronchial wash sample. It was observed that the NSCLC participants who had high serum based miR-194 expression showed 22 months of overall median survival while low expression of serum based miR-194 expression showed 18 months of overall median survival. Present study suggests that decreased expression of miR-194 and miR-192 was significantly associated with different clinical features of NSCLC cases. However, significantly higher number of NSCLC cases showed low expression of miR-194 and miR-192 in bronchial lavage sample. Decreased poor overall survival was found to be associated with bronchial wash sample with respect to low miR-194 and miR-192 expression while NSCLC participants showed better overall survival with high miR-194 and miR-192 expression. This suggested decreased expression of miR-192 and miR-194 expression could be the potential prognostic marker among NSCLC participants.

show abstract

Intracellular ROS scavenging and antioxidant regulation of WL15 from cysteine and glycine-rich protein 2 demonstrated in zebrafish in vivo model

Cited by 74 publications

References 57 publications

RM12 similar to substance P from tachykinin of freshwater murrel Channa striatus influence intracellular ROS in vitro fish erythrocytes and developmental toxicity and antioxidant enzymes in vivo zebrafish embryo

RM12 similar to substance P from tachykinin of freshwater murrel Channa striatus influence intracellular ROS in vitro fish erythrocytes and developmental toxicity and antioxidant enzymes in vivo zebrafish embryo

Neurokinin-1-tachykinin receptor agonist promotes diabetic fracture healing in rats with type 1 diabetes via modulation of Wnt/β-catenin signalling axis

Dysregulation of exosomal miR-192 and miR-194 expression in lung adenocarcinoma patients

Contact Info

Product

Resources

About