Intracellular Signaling Pathways Integrating the Pore Associated with P2X7R Receptor with Other Large Pores

Ferreira, L. G.; Reis, Rodrigo S.; Alves, LA; Faria, Robson Xavier

doi:10.5772/38842

Cited by 5 publications

(2 citation statements)

References 150 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Different effects of vitamin D 3 on P2X 7 channels and pores in CKD patients may have been made due to different sensitivity to these channels and pores. It is known that various agonists and antagonists of P2X 7 receptors may have a distinct effect on the function of P2X 7 channels and/or pores [ 37 ]. Not only the function but also the expression may be altered in some pathological conditions [ 38 – 41 ].…”

Section: Discussionmentioning

confidence: 99%

The Impact of Vitamin D₃Supplementation on Mechanisms of Cell Calcium Signaling in Chronic Kidney Disease

Lajdova

Spustová

Oksa

et al. 2015

BioMed Research International

View full text Add to dashboard Cite

Intracellular calcium concentration in peripheral blood mononuclear cells (PBMCs) of patients with chronic kidney disease (CKD) is significantly increased, and the regulatory mechanisms maintaining cellular calcium homeostasis are impaired. The purpose of this study was to examine the effect of vitamin D3 on predominant regulatory mechanisms of cell calcium homeostasis. The study involved 16 CKD stages 2-3 patients with vitamin D deficiency treated with cholecalciferol 7000–14000 IU/week for 6 months. The regulatory mechanisms of calcium signaling were studied in PBMCs and red blood cells. After vitamin D3 supplementation, serum concentration of 25(OH)D3 increased (P < 0.001) and [Ca2+]i decreased (P < 0.001). The differences in [Ca2+]i were inversely related to differences in 25(OH)D3 concentration (P < 0.01). Vitamin D3 supplementation decreased the calcium entry through calcium release activated calcium (CRAC) channels and purinergic P2X7 channels. The function of P2X7 receptors was changed in comparison with their baseline status, and the expression of these receptors was reduced. There was no effect of vitamin D3 on P2X7 pores and activity of plasma membrane Ca2+-ATPases. Vitamin D3 supplementation had a beneficial effect on [Ca2+]i decreasing calcium entry via CRAC and P2X7 channels and reducing P2X7 receptors expression.

show abstract

Section: Discussionmentioning

confidence: 99%

The Impact of Vitamin D₃Supplementation on Mechanisms of Cell Calcium Signaling in Chronic Kidney Disease

Lajdova

Spustová

Oksa

et al. 2015

BioMed Research International

View full text Add to dashboard Cite

show abstract

“…Different effects of vitamin D 3 on P2X 7 channels and pores in CKD patients may have been made due to different sensitivity to these channels and pores. It is known, that various agonists and antagonists of P2X 7 receptors may have a distinct effect on the function of P2X 7 channels and/or pores [35]. Not only the function, but also the expression of P2X 7 receptors may be altered in some pathological conditions [26,[36][37][38].…”

Section: P2x 7 Receptorsmentioning

confidence: 99%

Calcium Transport across Plasma Membrane in Early Stages of Chronic Kidney Disease – Impact of Vitamin D3 Supplementation

Lajdova¹,

Spustová²

2015

J Kidney

View full text Add to dashboard Cite

Calcium cations (Ca 2+) play an important role in the cellular signaling and in a number of physiological processes. The cell Ca 2+ distribution is exactly regulated by transport systems which maintain low free cytosolic calcium concentration ([Ca 2+ ] i) (50-100 nmol/l) in comparison with Ca 2+ concentration in extracellular space (about 1.3 mmol/l). The plasma membrane possesses several types of channels which control the Ca 2+ entry into the cells, and systems that extrude it. Calcium enters the cell by any of the general classes of channels, including voltage-operated channels (VOC), second messengeroperated channels (SMOC), store-operated channels (SOC) and receptor operated-channels (ROC). Calcium release from the cells is regelated principally by Ca 2+-ATPase, and Na + exchanger (Figure 1). Plasma membrane is responsible for the 10,000-fold gradient of Ca 2+ normally measured between the extracellular space and the cytoplasm. The very large gradient of Ca 2+ across the plasma membrane not only ensures full availability of the cation, but it is also dynamically beneficial, since even minor changes in the Ca 2+ permeability of the plasma membrane provides significant fluctuations in the [Ca2 + ]i. Passing Ca 2+ through the plasma membrane triggers a cascade of events that are vital for cellular activities. Calcium cation is an important ubiquitous messenger, controlling a broad range of cellular processes. It has a direct role in controlling the expression patterns of its signaling systems that are constantly being remodelled in both, health and disease.

show abstract

Fluorescent dyes as a reliable tool in P2X7 receptor-associated pore studies

Ferreira

Pereira

Faria

2015

J Bioenerg Biomembr

View full text Add to dashboard Cite

Since the nineteenth century, a great amount of different biological structures and processes have been assessed by fluorescent dyes. Along with the uses of these compounds as vital and histological dyes, some fluorescent dyes have become valuable tools for the study of the pore phenomenon in plasma membranes. Some ion channels capable of forming large conductance channels, such as P2X7, TRPV1, VDAC-1 and the maxi-anion channels transiently alter the plasma membrane permeability, producing pores, which permit the passage of molecules of up to 1,000 Da. In this review, we discuss the uses of the fluorescent dyes chosen in diverse studies of this topic up to now.

show abstract

Intracellular Signaling Pathways Integrating the Pore Associated with P2X7R Receptor with Other Large Pores

Cited by 5 publications

References 150 publications

The Impact of Vitamin D₃Supplementation on Mechanisms of Cell Calcium Signaling in Chronic Kidney Disease

The Impact of Vitamin D₃Supplementation on Mechanisms of Cell Calcium Signaling in Chronic Kidney Disease

Calcium Transport across Plasma Membrane in Early Stages of Chronic Kidney Disease – Impact of Vitamin D3 Supplementation

Fluorescent dyes as a reliable tool in P2X7 receptor-associated pore studies

Contact Info

Product

Resources

About

Intracellular Signaling Pathways Integrating the Pore Associated with P2X7R Receptor with Other Large Pores

Cited by 5 publications

References 150 publications

The Impact of Vitamin D3Supplementation on Mechanisms of Cell Calcium Signaling in Chronic Kidney Disease

The Impact of Vitamin D3Supplementation on Mechanisms of Cell Calcium Signaling in Chronic Kidney Disease

Calcium Transport across Plasma Membrane in Early Stages of Chronic Kidney Disease – Impact of Vitamin D3 Supplementation

Fluorescent dyes as a reliable tool in P2X7 receptor-associated pore studies

Contact Info

Product

Resources

About

The Impact of Vitamin D₃Supplementation on Mechanisms of Cell Calcium Signaling in Chronic Kidney Disease

The Impact of Vitamin D₃Supplementation on Mechanisms of Cell Calcium Signaling in Chronic Kidney Disease