Intracellular spectral recompositioning of light enhances algal photosynthetic efficiency

Fu, Weiqi; Chaiboonchoe, Amphun; Khraiwesh, Basel; Sultana, Mehar; Jaiswal, Ashish; Jijakli, Kenan; Nelson, David R.; Al-Hrout, Ala’a; Baig, Badriya; Amin, Amr; Salehi‐Ashtiani, Kourosh

doi:10.1126/sciadv.1603096

Cited by 48 publications

(29 citation statements)

References 64 publications

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“…Among the condition factors, light and nutrient have been reported to take charge in the important physiological and morphological responses 13 . Though light is essential for photosynthesis, the solarto-biomass conversion efficiency is <6% for most photosynthetic organisms 4,14 . Up till now, light intensity, light quality, and light cycle have been tested in order to confirm their best implementation in cell growth, reproduction and accumulation of highvalue products 15 .…”

mentioning

confidence: 99%

Harnessing C/N balance of Chromochloris zofingiensis to overcome the potential conflict in microalgal production

et al. 2020

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Accumulation of high-value products in microalgae is not conducive with rapid cell growth, which is the potential conflict in microalgal production. Overcoming such conflict faces numerous challenges in comprehensively understanding cell behavior and metabolism. Here, we show a fully integrated interaction between cell behavior, carbon partitioning, carbon availability and path rate of central carbon metabolism, and have practically overcome the production conflict of Chromochloris zofingiensis. We demonstrate that elevated carbon availability and active path rate of precursors are determinants for product biosynthesis, and the former exhibits a superior potential. As protein content reaches a threshold value to confer survival advantages, carbon availability becomes the major limiting factor for product biosynthesis and cell reproduction. Based on integrated interaction, regulating the C/N balance by feeding carbon source under excess light increases content of high-value products without inhibiting cell growth. Our findings provide a new orientation to achieve great productivity improvements in microalgal production.

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confidence: 99%

Harnessing C/N balance of Chromochloris zofingiensis to overcome the potential conflict in microalgal production

et al. 2020

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“…The engineering of photosystem II protein D1 isoform in Chlamydomonas showed enhanced photosynthetic efficiency under saturating light conditions (Vinyard et al, 2014 ). In another novel approach, the diatom P. tricornutum was engineered to establish a concept of intracellular spectral recompositioning for improved light absorption and consequent higher biomass production (Fu et al, 2017 ). In this case, the overexpressed green fluorescent protein absorbs the excess blue light energy from incident light and subsequently emits energy as green light that can be harvested by accessory pigments.…”

Section: Case Studies For Genetic Engineering In Microalgaementioning

confidence: 99%

Bioengineering of Microalgae: Recent Advances, Perspectives, and Regulatory Challenges for Industrial Application

Kumar

Shekh

Jakhu

et al. 2020

Front. Bioeng. Biotechnol.

194

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Microalgae, due to their complex metabolic capacity, are being continuously explored for nutraceuticals, pharmaceuticals, and other industrially important bioactives. However, suboptimal yield and productivity of the bioactive of interest in local and robust wild-type strains are of perennial concerns for their industrial applications. To overcome such limitations, strain improvement through genetic engineering could play a decisive role. Though the advanced tools for genetic engineering have emerged at a greater pace, they still remain underused for microalgae as compared to other microorganisms. Pertaining to this, we reviewed the progress made so far in the development of molecular tools and techniques, and their deployment for microalgae strain improvement through genetic engineering. The recent availability of genome sequences and other omics datasets form diverse microalgae species have remarkable potential to guide strategic momentum in microalgae strain improvement program. This review focuses on the recent and significant improvements in the omics resources, mutant libraries, and high throughput screening methodologies helpful to augment research in the model and non-model microalgae. Authors have also summarized the case studies on genetically engineered microalgae and highlight the opportunities and challenges that are emerging from the current progress in the application of genome-editing to facilitate microalgal strain improvement. Toward the end, the regulatory and biosafety issues in the use of genetically engineered microalgae in commercial applications are described.

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“…One of these strategies is the use of a high silicate medium along with blue light under high light conditions (255 µE m −2 s −1 ) for fucoxanthin production in P. tricornutum [ 145 ]. Another is the application of intracellular spectral recompositioning of light (ISR) on a genetically engineered P. tricornutum , with a green fluorescent protein (GFP) to enhance photosynthetic efficiency (by 50%) and biomass productivity, influencing fucoxanthin production in diatoms [ 146 ]. In addition, enhanced lipid production in genetically modified P. tricornutum stable strains has been achieved by the use of gene editing tools such as meganucleases and transcription activator-like effector nucleases (TALEN) to achieve targeted disruption of the UDP-glucose pyrophosphorylase gene, a step involved in carbohydrate accumulation that enabled routing carbon to lipid accumulation [ 147 ].…”

Section: Carbon Assimilation In Diatomsmentioning

confidence: 99%

Diatoms for Carbon Sequestration and Bio-Based Manufacturing

Sethi

Butler

Shuhaili

et al. 2020

Biology

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Carbon dioxide (CO2) is a major greenhouse gas responsible for climate change. Diatoms, a natural sink of atmospheric CO2, can be cultivated industrially in autotrophic and mixotrophic modes for the purpose of CO2 sequestration. In addition, the metabolic diversity exhibited by this group of photosynthetic organisms provides avenues to redirect the captured carbon into products of value. These include lipids, omega-3 fatty acids, pigments, antioxidants, exopolysaccharides, sulphated polysaccharides, and other valuable metabolites that can be produced in environmentally sustainable bio-manufacturing processes. To realize the potential of diatoms, expansion of our knowledge of carbon supply, CO2 uptake and fixation by these organisms, in conjunction with ways to enhance metabolic routing of the fixed carbon to products of value is required. In this review, current knowledge is explored, with an evaluation of the potential of diatoms for carbon capture and bio-based manufacturing.

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Intracellular spectral recompositioning of light enhances algal photosynthetic efficiency

Abstract: Spectral conversion of light enhances algal photosynthesis and enables technologies for sustainable energy and food production.

Cited by 48 publications

References 64 publications

Harnessing C/N balance of Chromochloris zofingiensis to overcome the potential conflict in microalgal production

Harnessing C/N balance of Chromochloris zofingiensis to overcome the potential conflict in microalgal production

Bioengineering of Microalgae: Recent Advances, Perspectives, and Regulatory Challenges for Industrial Application

Diatoms for Carbon Sequestration and Bio-Based Manufacturing

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