Intracellular survival of Brucella abortus, Mycobacterium bovis BCG, Salmonella dublin, and Salmonella typhimurium in macrophages from cattle genetically resistant to Brucella abortus

Qureshi, Tariq; Templeton, Joe W.; Adams, L. Garry

doi:10.1016/0165-2427(95)05492-8

Cited by 66 publications

(59 citation statements)

References 23 publications

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“…Clinical manifestations and immunopathological diversities in the course of the disease are dependent on the properties of the pathogen [7,10,22,25]. The most common serotypes in cattle are Salmonella(S) Typhimurium and S. Dublin [6,22]. Uncommon serotypes such as S. Infantis and S. Heidelberg in endemic and epizootic outbreaks of bovine salmonellosis have been also recently reported [1,8,14].…”

mentioning

confidence: 99%

“…Bovine salmonellosis, caused by infection with Salmonellae belonging to an intracellular facultative and Gramnegative bacterium, is an economically important disease as well as a public health concern in many countries [6,7,22]. In adult cattle, salmonellosis causes abortion and severe diarrhea, which can result in death [7,14].…”

mentioning

confidence: 99%

“…In adult cattle, salmonellosis causes abortion and severe diarrhea, which can result in death [7,14]. Clinical manifestations and immunopathological diversities in the course of the disease are dependent on the properties of the pathogen [7,10,22,25]. The most common serotypes in cattle are Salmonella(S) Typhimurium and S. Dublin [6,22].…”

mentioning

confidence: 99%

“…To date, research on bovine salmonellosis has focused on the biological and molecular bacteriology for vaccine strategies [12,22,27,29]. There have been limited informations on the pathogenesis including the roles of endotoxin and cytokines in this disease [16,18,19,25].…”

mentioning

confidence: 99%

“…Endotoxin, which is a structural part of the outer cell wall of Salmonellae, induces secretion of inflammatory cytokines such as tumor necrosis factor-α (TNF-α), interleukin(IL)-1, and IL-6 by macrophages [7,16,18,25]. It is now accepted that pathodynamic and clinical symptoms caused by intracellular facultative and Gram-negative bacteria depend on interactions between cytokines and immune cells [19,20,22,23,25,28]. The pivotal role of immune cells in these responses has been demonstrated most clearly in mouse models [11,13,25,[27][28][29].…”

mentioning

confidence: 99%

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Inflammatory Cytokines and Antigen-Responsive Mononuclear Cells in Peripheral Blood of Cattle Infected with Salmonella Takoradi.

Konnai

Htsuka

Sakamoto³

et al. 2001

The Journal of Veterinary Medical Science

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ABSTRACT. To determine the immunological response in lactating dairy cows infected with Salmonella (S.) Takoradi, the relationships among distributions of peripheral blood mononuclear cell (PBMC) subpopulations, endotoxin concentrations and dynamics of inflammatory cytokines in blood were investigated. The ratio of CD4 + T cells to CD8 + T cells was significantly lower in the affected cattle than in the control cattle (p<0.05) to decrease in the number of CD4 + T cells in the infected cattle. In contrast, the numbers of γδ-T cells, MHC class II-positive cells were significantly higher in the affected cattle than in the control cattle (p<0.01 respectively). Endotoxemia was found in all but one of the affected cattle. Serum IL-1 and IL-6 bioactivities were significantly higher in the affected cattle than in the control cattle (IL-1, p<0.05; IL-6, p<0.01). Serum TNF-α activities and levels were not detected in the control and affected cattle. The activities of proinflammatory cytokines determined by the bioassay are important to the relationships between concentration of endotoxin, cytokines and clinical signs, such as leukocytosis, leukopenia, fever or bacterial shedding. Serum IL-2 levels were lower in the affected cattle than in the control cattle. Serum IFN-γ was not detected in the affected cattle except one. These results by the ELISA seemed to reflect the condition of subpopulation in the PBMCs from the shedding cattle. The present results suggest that cellular immunity is suppressed while the humoral immunity is activated in acute bovine salmonellosis. KEY WORDS: bovine salmonellosis, cytokine, endotoxin, PBMC.

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