Intracellular survival of Clostridium chauvoei in bovine macrophages

Pires, Prhiscylla Sadanã; Santos, Renato L.; Paixão, Tatiane Alves da; Bernardes, Laura Cristina de Oliveira; Macêdo, Auricélio A.; Gonçalves, Luciana Aramuni; Júnior, Carlos Augusto de Oliveira; Silva, Rodrigo; Lobato, Francisco Carlos Faria

doi:10.1016/j.vetmic.2016.11.027

Cited by 16 publications

(19 citation statements)

References 24 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…A recent study showed that the vegetative and sporulated forms of C. chauvoei are able to resist the microbicidal effects of macrophages in murine and bovine monocyte-derived macrophages, supporting the importance of macrophages during the early pathogenesis of blackleg (PIRES et al, 2017). These authors also noted a pro-inflammatory cytokine profile such as IL-12 and IL-23 transcription in bovine macrophages after infection with vegetative C. chauvoei.…”

Section: Pathogenesismentioning

confidence: 81%

“…These authors also noted a pro-inflammatory cytokine profile such as IL-12 and IL-23 transcription in bovine macrophages after infection with vegetative C. chauvoei. Conversely, in bovine macrophages infected with the spores of C. chauvoei, an antiinflammatory cytokine profile such as induction of IL-10 and TGF-beta transcription was observed (PIRES et al, 2017). The anti-inflammatory profile induced by spores might explain their latency after macrophage internalization.…”

Section: Pathogenesismentioning

confidence: 96%

See 1 more Smart Citation

Blackleg in cattle: current understanding and future research needs

et al. 2018

View full text Add to dashboard Cite

Blackleg is an endogenous acute infection that principally affects cattle, whose etiologic agent is the anaerobic bacterium Clostridium chauvoei. In recent years, the major virulence factors of C. chauvoei have been discovered and described. However, the pathogenesis of blackleg in cattle, and in particular, the movement of the pathogen from the point of entry to the affected tissues is not yet fully elucidated. Disease control is based on appropriate management and vaccination. This review summarizes the latest research findings that contribute toward the understanding of the disease in cattle, provide a foundation to preventive strategies, and identify future research needs.

show abstract

Section: Pathogenesismentioning

confidence: 81%

Section: Pathogenesismentioning

confidence: 96%

Blackleg in cattle: current understanding and future research needs

et al. 2018

View full text Add to dashboard Cite

show abstract

“…What's more, C. cadaveris could trigger Bacteremia [61]. And C. chauvoei could cause blackleg of ruminant [62].…”

Section: Pathogenicity Of Clostridium Speciesmentioning

confidence: 99%

Clostridium species as probiotics: potentials and challenges

Guo

Zhang

et al. 2020

J Animal Sci Biotechnol

334

192

View full text Add to dashboard Cite

Clostridium species, as a predominant cluster of commensal bacteria in our gut, exert lots of salutary effects on our intestinal homeostasis. Up to now, Clostridium species have been reported to attenuate inflammation and allergic diseases effectively owing to their distinctive biological activities. Their cellular components and metabolites, like butyrate, secondary bile acids and indolepropionic acid, play a probiotic role primarily through energizing intestinal epithelial cells, strengthening intestinal barrier and interacting with immune system. In turn, our diets and physical state of body can shape unique pattern of Clostridium species in gut. In view of their salutary performances, Clostridium species have a huge potential as probiotics. However, there are still some nonnegligible risks and challenges in approaching application of them. Given this, this review summarized the researches involved in benefits and potential risks of Clostridium species to our health, in order to develop Clostridium species as novel probiotics for human health and animal production.

show abstract

“…The blackleg disease is causing huge economic losses and is a limiting factor in commercial dairy animals. Previous studies on blackleg infection usually revolved around molecular identification [18,19], and classical skeletal muscle pathology [20]. For diagnosis of blackleg infection, clinical ailments and necropsy lesions may be sufficient, however, histopathological and blood biochemical investigations in this study are useful for the understanding of pathogenesis, therapeutic procedures and control strategies of visceral blackleg in dairy animals.…”

Section: Discussionmentioning

confidence: 96%

Pathological and clinical investigations of an outbreak of Blackleg disease due to C. chauvoei in cattle in Punjab, Pakistan

Hussain

Javed

Khan

et al. 2019

J Infect Dev Ctries

View full text Add to dashboard Cite

Introduction: Clostridium chauvoei (C. chauvoei) is an anaerobic, histotoxic Gram-positive, bacterium causing fatal myonecrosis in livestock with high mortalities. The disease is common in dairy animals, but little is known about the pathophysiology of the disease in exotic (non-native) animals kept under local conditions in Pakistan. Methodology: Diagnosis of blackleg was made based on hematological and serum biochemical analysis, PCR, necropsy and histopathology. Results: Clinically sick animals exhibited fever, lameness, subcutaneous gaseous swelling and edema particularly in hindquarter and front legs. Hematological analysis showed increases in erythrocyte sedimentation rate and reduces in number of red blood cells, packed cell volume, leukocytes and differential leukocyte count. Serum aspartate aminotransferase, lactate dehydrogenase, alkaline phosphates, alanine aminotransferase, urea, creatinine, creatine kinase, and creatinine phosphokinase were significantly (P < 0.05) higher in the infected animals. At necropsy, swelling areas contained straw-colored fluid with gas bubbles. The muscles were swollen, dark to black and exhibited crepitation sounds at the time of incisions with a rancid odor. Severe pulmonary edema, myocarditis along with petechial hemorrhages, as well as enlargement and congestion of liver and spleen have been observed. Microscopic examination revealed severe inflammatory reaction, edema, and disruption of the myofibrils. Examination of heart, spleen, liver, kidneys, intestine, and lungs showed congestion, severe inflammatory changes with neutrophilic infiltration and necrosis accompanied by dissociation of the normal tissue structure. PCR confirmed C. chauvoei in exudates and different samples of muscles. Conclusion: The pathophysiology should be considered in diagnosis of blackleg. The disease is exist in the non-native cattle farms and biosecurity measures have to be elevated.

show abstract

Intracellular survival of Clostridium chauvoei in bovine macrophages

Cited by 16 publications

References 24 publications

Blackleg in cattle: current understanding and future research needs

Blackleg in cattle: current understanding and future research needs

Clostridium species as probiotics: potentials and challenges

Pathological and clinical investigations of an outbreak of Blackleg disease due to C. chauvoei in cattle in Punjab, Pakistan

Contact Info

Product

Resources

About