INTRACELLULAR SYNTHESIS, TRANSPORT, AND PACKAGING OF PROTEINACEOUS YOLK IN OOCYTES OF ORCONECTES IMMUNIS

Abstract

The incorporation of leucine-3 H into either ovarian or oocyte proteins occurs throughout vitellogenesis, but is at a maximum during early phases of this process . The labeling of ovarian and oocyte proteins is inhibited with cycloheximide . Oocytes are permeable to actinomycin D, and this drug does not affect the incorporation of amino acids into oocyte proteins but does block oocyte RNA synthesis . By means of both light microscope and high resolution radioautography, it has been demonstrated that the initial incorporation of leucine-3 H under both in vitro and in vivo conditions occurs in elements of the roughsurfaced endoplasmic reticulum in the oocyte . Under pulse-chase conditions, the label subsequently becomes associated with intracisternal (precursor yolk) granules now aggregated within the cisternae of the connected smooth-surfaced endoplasmic reticulum . By 7 days, mature yolk globules are extensively labeled . The results of experiments designed to assess the possible contribution of maternal blood proteins to yolk deposition indicate that such a contribution is minimal . It is concluded that the crayfish oocyte is programmed for and capable of synthesizing the massive store of proteinaceous yolk present in the egg at the end of oogenesis . 420

Life stages and reproductive components of the Marmorkrebs (marbled crayfish), the first parthenogenetic decapod crustacean

“…The oocytes are metabolically highly active during both primary and secondary vitellogenesis, as can be deduced from the abundance of rER, mitochondria, and Golgi bodies. Similar observations were also made in bisexual crayfish (Ganion and Kessel, 1972) or shrimp (Carvalho et al, 1998). Primary and secondary vitellogenic oocytes of crayfish differ from each other not only with respect to size and staining properties, as shown for the marbled crayfish, but also with regard to the yolk proteins (vitellins) they deposit.…”

“…This result, however, should be taken with care before being verified by the use of more refined histological and electron microscopic techniques. The architecture of the mature ovary of the marbled crayfish fits well into the generalized scheme given for crayfish ovaries (Ando and Makioka, 1998;Vogt, 2002) and shows no striking differences to bisexual crayfish with respect to anatomy, histology of oogenesis (Abdu et al, 2000), or ultrastructure of vitellogenesis (Ganion and Kessel, 1972). However, there is a difference regarding meiosis, which is apparently lacking in the marbled crayfish.…”

Vitellogenin, the extraovarian precursor of the protein yolk in Crustacea : A review

“…The second period of oocyte growth, or vitellogenesis strictly speaking (« secondary vitellogenesis » according to the terminology of Dhainaut and de Leersnyder, 1976a ;Charniaux-Cotton, 1978) Zerbib (1973Zerbib ( , 1978 (Beams and Kessel, 1963 ;Kessel, 1968a, b ;Ganion and Kessel, 1972) Busson-Mabillot, 1969). In all these groups, the general outline of the mode of yolk body formation was analogous to that described in 0. gammarella.…”

La vitellogénine chez un Crustacé Décapode Natantia, Palaemon serratus Pennant. Mise en évidence, comparaison immunologique avec les vitellines, site de synthèse et rôle des pédoncules oculaires

“…Ainsi, la vitellogenèse « secondaire » (Charniaux-Cotton, 1978) (Beams et Kessel, 1962Kessel, , 1963Kessel, 1968 ;Ganion et Kessel, 1972 (Junéra et et les Isopodes Porcellio dilatatus (Picaud, 1980) et ldotea balthica basteri (Souty et Picaud, 1981 (Junéra et al, 1977 ;Meusy, 1980 Lüscher, 1975 ;Gutzeit, 1980 ;etc...) O'Connor (1976O'Connor ( , 1977 appellent également quelques…”