Intracellular trafficking and gene expression of pH-sensitive, artificially enveloped adenoviruses in vitro and in vivo

Bossche, Johan Van den; Al-Jamal, Wafa' T; Yılmazer, Açelya; Bizzarri, Elisabetta; Tian, Bowen; Kostarelos, Kostas

doi:10.1016/j.biomaterials.2010.12.043

Cited by 35 publications

(22 citation statements)

References 27 publications

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“…In considering liposomes, we hypothesized that they would serve two primary functions: (i) as a barrier to protons (27), thus protecting phages against gastric acids, and (ii) as a promoter of mucoadhesiveness, owing to their positively charged surfaces (28,29), which would prolong the intestinal residence time. We chose cationic liposomes as the encapsulation matrix because they readily allow the encapsulation of negatively charged, nanoscale biological entities (30,31) such as phages. Furthermore, cationic liposomes favor the dispersion of their biomolecules in aqueous media over prolonged periods of time, facilitate the oral administration of these biomolecules to animals, and are degraded upon contact with intestinal bile salts (32).…”

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confidence: 99%

Liposome-Encapsulated Bacteriophages for Enhanced Oral Phage Therapy against Salmonella spp

Colom

Cano‐Sarabia

Otero

et al. 2015

Appl Environ Microbiol

159

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and UAB_Phi87 were encapsulated in liposomes, and their efficacy in reducing Salmonella in poultry was then studied. The encapsulated phages had a mean diameter of 309 to 326 nm and a positive charge between ؉31.6 and ؉35.1 mV (pH 6.1). In simulated gastric fluid (pH 2.8), the titer of nonencapsulated phages decreased by 5.7 to 7.8 log units, whereas encapsulated phages were significantly more stable, with losses of 3.7 to 5.4 log units. The liposome coating also improved the retention of bacteriophages in the chicken intestinal tract. When cocktails of the encapsulated and nonencapsulated phages were administered to broilers, after 72 h the encapsulated phages were detected in 38.1% of the animals, whereas the nonencapsulated phages were present in only 9.5%. The difference was significant. In addition, in an in vitro experiment, the cecal contents of broilers promoted the release of the phages from the liposomes. In broilers experimentally infected with Salmonella, the daily administration of the two cocktails for 6 days postinfection conferred similar levels of protection against Salmonella colonization. However, once treatment was stopped, protection by the nonencapsulated phages disappeared, whereas that provided by the encapsulated phages persisted for at least 1 week, showing the enhanced efficacy of the encapsulated phages in protecting poultry against Salmonella over time. The methodology described here allows the liposome encapsulation of phages of different morphologies. The preparations can be stored for at least 3 months at 4°C and could be added to the drinking water and feed of animals. Foodborne diseases are a global health problem and include infections with nontyphoidal Salmonella, which is among the most common zoonotic pathogens that affect humans. In fact, this bacterium was responsible for more than 1 million annual cases of food-related illnesses in the United States from 2000 to 2008 (1) and for more than 90,000 cases of salmonellosis (mainly from Salmonella enterica serovars Enteritidis and Typhimurium) diagnosed in the European Union in 2012 (2). The major source of Salmonella infections in humans is poultry products (2), and poultry is the major reservoir of this bacterium. The health risk is exacerbated in the case of broilers, which are asymptomatic carriers that house Salmonella in their gut. In fact, several Salmonella strains persistently colonize chickens but without causing any signs of illness (3). This lack of clinical symptoms facilitates the dissemination of Salmonella within flocks (4), thereby increasing the probability of cross-contamination during the transport, slaughter, and processing of broilers.Salmonella infection in broilers has been controlled mainly through the use of vaccines (5, 6), probiotics, prebiotics, and synbiotics (7), and antibiotics (8), although the effectiveness of these treatments is limited. Importantly, antibiotics can ultimately increase the severity and frequency of colonization by certain resistant strains of Salmonella (9).Phages are viruses ...

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confidence: 99%

Liposome-Encapsulated Bacteriophages for Enhanced Oral Phage Therapy against Salmonella spp

Colom

Cano‐Sarabia

Otero

et al. 2015

Appl Environ Microbiol

159

140

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“…With the development of nanotechnology, some nanomaterials including gold nanoparticles (AuNPs), quantum dots (QDs), superparamagnetic iron oxides (SPIOs) as well as viral‐nanoparticles have been hybridized with bilayer lipids for gene delivery in order to increase the efficiency or controllability of gene delivery . We have also synthesized AuNPs coated by dimethyldioctadecylammonium bromide (DODAB) served as the gene vector (DODAB‐AuNPs), which could significantly increase the transfection efficiency and decrease the cytotoxic effect of DODAB to the cells.…”

Section: Introductionmentioning

confidence: 99%

“…full papers www.MaterialsViews.com (QDs), superparamagnetic iron oxides (SPIOs) as well as viral-nanoparticles have been hybridized with bilayer lipids for gene delivery in order to increase the effi ciency or controllability of gene delivery. [22][23][24][25][26] We have also synthesized AuNPs coated by dimethyldioctadecylammonium bromide (DODAB) served as the gene vector (DODAB-AuNPs), [ 23 ] which could signifi cantly increase the transfection effi ciency and decrease the cytotoxic effect of DODAB to the cells. To further increase the transfection effi ciency and to meet with the desired features of gene vectors, such as good biocompatibility, high gene loading capability, terrifi c gene protective capability as well as sensitive and effective gene release ability, [27][28][29] a neutral lipid, dioleoylphosphatidylethanolamine (DOPE) was added to synthesize a new gene vector (DODAB/DOPE-AuNPs).…”

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Anionic Lipid, pH‐Sensitive Liposome‐Gold Nanoparticle Hybrids for Gene Delivery – Quantitative Research of the Mechanism

Tian

et al. 2015

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Gene therapy is a potential method for treating a large range of diseases. Gene vectors are widely used in gene therapy for promoting the gene delivery efficiency to the target cells. Here, gold nanoparticles (AuNPs) coated with dimethyldioctadecylammonium bromide (DODAB)/dioleoylphosphatidylethanolamine (DOPE) are synthesized using a facile method for a new gene vector (DODAB/DOPE-AuNPs), which possess 3- and 1.5-fold higher transfection efficiency than those of DODAB-AuNPs and a commercial transfection agent, respectively. Meanwhile, it is nontoxic with concentrations required for effective gene delivery. Imaging and quantification studies of cellular uptake reveal that DOPE increases gene copies in cells, which may be attributed to the smaller size of AuNPs/DNA complexes. The dissociation efficiency of DNA from the endocytic pathway is quantified by incubating with different buffers and investigated directly in the cells. The results suggest that DOPE increases the internalization of AuNPs/DNA complexes and promotes DNA release from early endosomes for the vector is sensitive to the anionic lipid membrane and the decreasing pH along the endocytic pathway. The new vector contains the potential to be the new alternative as gene delivery vector for biomedical applications.

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“…44–46 In addition, it is well known that the transfection efficiency of lipoplex can be boosted by incorporation of DOPE as a helper lipid in the formulation. 47–49 …”

Section: Resultsmentioning

confidence: 99%

Effective Targeted Gene Delivery to Dendritic Cells via Synergetic Interaction of Mannosylated Lipid with DOPE and BCAT

et al. 2012

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The efficient delivery of plasmids encoding antigenic determinants into dendritic cells (DCs) that control immune response is a promising strategy for rapid development of new vaccines. In this study, we prepared a series of targeted cationic lipoplex based on two synthetic lipid components, mannose-poly(ethylene glycol, MW3000)-1,2-distearoyl-sn-glycero-3-phosphoethanolamine (Mannose-PEG3000-DSPE) and O-(2R-1,2-di-O-(1'Z,9'Z-octadecadienyl)-glycerol)-3-N-(bis-2-aminoethyl)-carbamate (BCAT), that were formulated with 1,2-dioleoyl-sn-glycero-3-phosphoethanolamine (DOPE) for evaluation as non-viral vectors for transgene expression in DCs. First, we optimized the N:P ratio for maximum transfection and then screened the effects of mannose targeting for further enhancement of transfection levels. Our results indicate that efficient delivery of gWIZ GFP plasmid into DCs was observed for mannose compositions of ~10%, whereas low transfection efficiencies were observed with non-targeted formulations. Mannose-targeted lipofectamine complexes also showed high GFP expression levels in DCs relative to non-targeted lipofectamine controls. The best transfection performance was observed using 10 mol % Mannose-PEG3000-DSPE, 60 mol% BCAT, and 30 mol % DOPE, indicating that the most efficient delivery into DCs occurs via synergistic interaction between mannose targeting and acid-labile, fusogenic BCAT:DOPE formulations. Our data suggest that mannose-PEG3000-DSPE:BCAT:DOPE formulations may be effective gene delivery vehicles for the development of DC-based vaccines.

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Intracellular trafficking and gene expression of pH-sensitive, artificially enveloped adenoviruses in vitro and in vivo

Cited by 35 publications

References 27 publications

Liposome-Encapsulated Bacteriophages for Enhanced Oral Phage Therapy against Salmonella spp

Liposome-Encapsulated Bacteriophages for Enhanced Oral Phage Therapy against Salmonella spp

Anionic Lipid, pH‐Sensitive Liposome‐Gold Nanoparticle Hybrids for Gene Delivery – Quantitative Research of the Mechanism

Effective Targeted Gene Delivery to Dendritic Cells via Synergetic Interaction of Mannosylated Lipid with DOPE and BCAT

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