Intracellular Transactivation of the Insulin-like Growth Factor I Receptor by an Epidermal Growth Factor Receptor

Burgaud, Jean-Luc; Baserga, Renato

doi:10.1006/excr.1996.0096

Cited by 55 publications

(32 citation statements)

References 0 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Although there was no significant association between coexpression of these proteins and overall survival, previous studies have suggested that interactions among families of growth factor receptors augment the malignant behavior of tumor cells (8,34). Furthermore, cross-talk between IGF-IR and EGFR or HER2 has been implicated in the development of tumor cell resistance to therapy with EGFR inhibitors and anti-HER2 monoclonal antibodies (35,36).…”

Section: Discussionmentioning

confidence: 97%

Impact of Insulin-Like Growth Factor Type 1 Receptor, Epidermal Growth Factor Receptor, and HER2 Expressions on Outcomes of Patients with Gastric Cancer

Matsubara¹,

Yamada²,

Hirashima³

et al. 2008

Clinical Cancer Research

106

View full text Add to dashboard Cite

Purpose: Expression levels of insulin-like growth factor type 1 receptor (IGF-IR), epidermal growth factor receptor (EGFR), and HER2 expressions have been linked to clinical outcomes in several solid tumors. However, the clinical significance of these biomarkers in gastric cancer (GC) remains unclear. This study was designed to delineate the clinical implications of these three biomarkers in GC. Experimental Design: The study group comprised 87 patients who underwent gastrectomy at National Cancer Center Hospital and subsequently received chemotherapy for recurrent or residual tumors. Using immunohistochemical techniques, we analyzed the expressions of IGF-IR, EGFR, and HER2 on formalin-fixed paraffin-embedded specimens of surgically removed primary tumors.Results: IGF-IR expression (defined as >10% membranous staining) was found in 67 tumors (77%), EGFR expression in 55 (63%), and HER2 expression in 16 (18%). Positive coexpression of IGF-IR and EGFR was found in 48 tumors (55%), that of IGF-IR and HER2 in16 (18%), and that of EGFR and HER2 in 13 (15%). Multivariate survival analysis showed that IGF-IR^positive expression [hazard ratio (HR) 2.14, 95% confidence interval (95% CI) 1.20-3.82; P = 0.01], performance status 1 or 2 (HR 1.83, 95% CI 1.15-2.91; P = 0.01), and diffuse type tumors (HR 1.71; 95% CI 1.08-2.70; P = 0.02) were significant predictors of poor survival. Conclusions: IGF-IR expression in surgical GC specimens, poor performance status, and diffuse type tumors are significant predictors of poor outcomes in patients with GC. Our data suggest that anti^IGF-IR strategies may prove valuable in such patients.

show abstract

Section: Discussionmentioning

confidence: 97%

Impact of Insulin-Like Growth Factor Type 1 Receptor, Epidermal Growth Factor Receptor, and HER2 Expressions on Outcomes of Patients with Gastric Cancer

Matsubara¹,

Yamada²,

Hirashima³

et al. 2008

Clinical Cancer Research

106

View full text Add to dashboard Cite

show abstract

“…The cooperative effects of EGF and IGF-I in embryo development may be anticipated, since EGF receptors can transactivate IGF-I receptors to stimulate mitogenesis in a murine embryonic cell line [47]. In experiment 3, we used the concentrations of 100 ng/ml EGF and 50 ng/ml IGF-I, since these concentrations of growth factors showed a significant effect on embryo development when compared with the controls in our culture system (Tables 1 and 2).…”

Section: Discussionmentioning

confidence: 99%

“…These findings suggest that a physiologically optimal concentration of IGF-I for bovine embryo development remains to be determined. The minimum concentration of IGF-I needed to obtain a maximum effect on embryo development may be 50 ng/ml in our culture system.The cooperative effects of EGF and IGF-I in embryo development may be anticipated, since EGF receptors can transactivate IGF-I receptors to stimulate mitogenesis in a murine embryonic cell line [47]. In experiment 3, we used the concentrations of 100 ng/ml EGF and 50 ng/ml IGF-I, since these concentrations of growth factors showed a significant effect on embryo development when compared with the controls in our culture system (Tables 1 and 2).…”

mentioning

confidence: 99%

Normal Calves Produced After Transfer of Embryos Cultured in a Chemically Defined Medium Supplemented with Epidermal Growth Factor and Insulin-like Growth Factor I Following Ovum Pick Up and <i>In Vitro</i> Fertilization in Japanese Black Cows

Sakagami

Umeki

Nishino³

et al. 2012

J. Reprod. Dev.

View full text Add to dashboard Cite

Abstract. The objective of this study was to examine whether high concentrations of epidermal growth factor (EGF) and/or insulin-like growth factor I (IGF-I) would have a beneficial effect on bovine embryo development in vitro and to obtain normal calves by using an ovum pick up method and embryo culture in a chemically defined medium. When compared with controls, EGF (100 or 200 ng/ml) or IGF-I (50 or 100 ng/ml) significantly increased the rate of embryos that developed into blastocysts during an 8-day culture after the in vitro fertilization of oocytes obtained from ovaries from a slaughterhouse. IGF-I induced a dose-dependent increase in cell number in both the inner cell mass and the trophectoderm, whereas EGF stimulated proliferation only in the inner cell mass. A combination of EGF (100 ng/ml) and IGF-I (50 ng/ml) produced an additive effect, and embryos developed into blastocysts at a comparatively high rate (27.9%) compared with controls (12.0%). A similar rate of development was achieved using a combination of EGF and IGF-I in the culture of embryos following ovum pick up by ultrasound-guided transvaginal follicular aspiration and in vitro fertilization, and 5 blastocysts that developed after the culture were transferred into uteri; two embryos implanted, and normal calves were born. These results suggest that the combined use of EGF and IGF-I makes bovine embryo culture in a chemically defined medium a practical and useful procedure for producing blastocysts, and its application to embryo culture following ovum pick up and in vitro fertilization could be useful for producing normal calves. Key words: Bovine embryo, Chemically defined medium, Epidermal growth factor (EGF), Insulin-like growth factor I (IGF-I), Ovum pick up (OPU) (J. Reprod. Dev. 58: [140][141][142][143][144][145][146] 2012) R ecently, a number of calves have been produced by the ovum pick up (OPU) method and in vitro culture (IVC) systems. Since the OPU method can be repeatedly applied to the same cow and can be used in pregnant [1] or problem cows in which embryos have not been obtained by uterine flushing after artificial insemination [2], this technology is effective for facilitating the improvement of domestic animals.An IVC system is necessary for the OPU method. Since successful pregnancy by transfer of embryos following the OPU method and in vitro fertilization has been confirmed [3], different protocols and medium conditions for oocyte maturation and embryo development have been tested [4][5][6][7]. However, there are some problems associated with the IVC system, such as a skewed sex ratio [8,9] or large offspring syndrome [10,11]. It has been suggested that factors in the culture medium such as serum used for embryo development may be involved in these problems. Fetal bovine serum (FBS) is widely employed as a supplement in the media used for culturing in vitro fertilized bovine embryos, but the subsequent rate of development of the fertilized oocytes to the blastocyst stage is known to vary among serum lots. There is thus a...

show abstract

“…These data suggested that tissue levels of IGF-I, and presumably, the activation state of the IGF-IR could influence the activation of EGFR. A number of mechanisms have recently been proposed whereby cross-talk between the EGFR and IGF-IR may occur (35)(36)(37)(38)(39). Current experiments are exploring possible mechanisms whereby reduced circulating IGF-I levels may disrupt cross-talk between the IGF-IR and EGFR.…”

Section: Discussionmentioning

confidence: 99%

Reduced Susceptibility to Two-Stage Skin Carcinogenesis in Mice with Low Circulating Insulin-Like Growth Factor I Levels

et al. 2008

View full text Add to dashboard Cite

show abstract

Intracellular Transactivation of the Insulin-like Growth Factor I Receptor by an Epidermal Growth Factor Receptor

Cited by 55 publications

References 0 publications

Impact of Insulin-Like Growth Factor Type 1 Receptor, Epidermal Growth Factor Receptor, and HER2 Expressions on Outcomes of Patients with Gastric Cancer

Impact of Insulin-Like Growth Factor Type 1 Receptor, Epidermal Growth Factor Receptor, and HER2 Expressions on Outcomes of Patients with Gastric Cancer

Normal Calves Produced After Transfer of Embryos Cultured in a Chemically Defined Medium Supplemented with Epidermal Growth Factor and Insulin-like Growth Factor I Following Ovum Pick Up and <i>In Vitro</i> Fertilization in Japanese Black Cows

Reduced Susceptibility to Two-Stage Skin Carcinogenesis in Mice with Low Circulating Insulin-Like Growth Factor I Levels

Contact Info

Product

Resources

About