Intracellular Transport and Degradation of¹²⁵I-Tyramine Cellobiose-Labelled Low-Density Lipoprotein Endocytosed in vivo in Rat Liver Cells Studied by Means of Subcellular Fractionation

Abstract

Enzymes:Acid phosphatase, orthophosphoric-monoester phosphohydrolase (acid optimum) (EC 3.1.3.2); TV-Acetyl-ß-glucosaminidase, ./V-acetyl-ß-D-glucosaminide ./V-acetylglucosaminohydrolase (EC 3.2.1.30); Glucose-6-phosphatase, D-glucose-6-phosphate phosphohydrolase (EC 3.1.3.9); 5'-Nucleotidase, 5'-ribonucleotide phosphohydrolase (EC 3.1.3.5). -Abbreviations:Apo, apolipoprotein; 125 I-TC-LDL, 125 I-tyramine cellobiose-labelled low density lipoprotein; PVP, poly(vinylpyrrolidone); CURL, compartment for uncoupling of receptor and ligand; MVB, multivesicular body; Hepes, 4-(2-hydroxyethyl)-l-piperazineethanesulfonic acid.