Intracellular uptake of EGCG-loaded deformable controlled release liposomes for skin cancer

Marwah, Mandeep; Perrie, Yvonne; Badhan, Raj; Lowry, Deborah

doi:10.1080/08982104.2019.1604746

Cited by 34 publications

(16 citation statements)

References 88 publications

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“…In contrast, the size of L:SC:PBS and L:PEG:PBS liposomes did not change, resulting in a deformability ratio of approximately 1. This shows that both PEGylated and sodium cholate liposomes are flexible, passing through pores smaller than their size and regaining their original size after the extrusion …”

Section: Results and Discussionmentioning

confidence: 87%

“…This shows that both PEGylated and sodium cholate liposomes are flexible, passing through pores smaller than their size and regaining their original size after the extrusion. 35 In Vitro Release of MSlys from Liposomes. The release of MSlys from the different liposomal formulations to PBS medium was evaluated using a cellulose ester membrane that allowed the separation of the released (free) endolysin.…”

Section: ■ Results and Discussionmentioning

confidence: 99%

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Sustained Release of a Streptococcus pneumoniae Endolysin from Liposomes for Potential Otitis Media Treatment

et al. 2021

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Local delivery of antimicrobials for otitis media treatment would maximize therapeutic efficacy while minimizing side effects. However, drug transport across the tympanic membrane in the absence of a delivery system is challenging. In this study, the MSlys endolysin was encapsulated in deformable liposomes for a targeted treatment of S. pneumoniae, one of the most important causative agents of otitis media. MSlys was successfully encapsulated in liposomes composed of l-alpha-lecithin and sodium cholate (5:1) or l-alpha-lecithin and PEG2000 PE (10:1), with encapsulation efficiencies of about 35%. The PEGylated and sodium cholate liposomes showed, respectively, mean hydrodynamic diameters of 85 and 115 nm and polydispersity indices of 0.32 and 0.42, both being stable after storage at 4 °C for at least one year. Both liposomal formulations showed a sustained release of MSlys over 7 days. Cytotoxicity studies against fibroblast and keratinocyte cell lines revealed the biocompatible nature of both MSlys and MSlys-loaded liposomes. Additionally, the encapsulated MSlys showed prompt antipneumococcal activity against planktonic and biofilm S. pneumoniae, thus holding great potential for transtympanic treatment against S. pneumoniae otitis media.

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Section: Results and Discussionmentioning

confidence: 87%

Section: ■ Results and Discussionmentioning

confidence: 99%

Sustained Release of a Streptococcus pneumoniae Endolysin from Liposomes for Potential Otitis Media Treatment

et al. 2021

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“…The mechanical property of NPs is another important factor in determining the endocytosis process, as proved by extensive experimental research [54][55][56]. However, there are often seemly controversial results regarding the effect of NP elasticity.…”

Section: Size and Elasticitymentioning

confidence: 99%

Interplay of Nanoparticle Properties during Endocytosis

Wang

Song

2021

Crystals

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Nanoparticles (NPs) have been widely applied as drug carriers in drug delivery, due to their unique physical and structural properties. To achieve the drug delivery purpose, receptor-mediated endocytosis is a primary explored mechanism to internalize NPs into tumor cells. During the endocytosis process, properties of NPs, including size, shape, and surface functionality, play an important role in determining the final drug delivery efficacy. Many of these NP properties have been extensively explored individually. However, the multiple NP properties naturally interplay with each other in the endocytosis process to determine the internalization efficiency together. Therefore, it is significantly important to understand the interplay of different NP properties to improve the NP’s final delivery efficacy. In this review, we focus on the interplay of NPs properties on the endocytosis process to summarize the relevant experimental observations and physical mechanisms. Particularly, three different aspects are discussed in detail, including the interplay between size and shape; size and elasticity; shape and elasticity. We have summarized the most recent works and highlighted that building up systematic understandings for the complex interplay between NP properties can greatly help a better design of NP platforms for drug delivery.

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“…Other studies indicate a stronger cellular uptake of EGCG through the use of carrier nanoparticles [ 24 , 25 ]. In addition, liposomes can be superficially modified to increase EGCG cellular uptake [ 23 , 26 ].…”

Section: Discussionmentioning

confidence: 99%

Cellular Uptake of Epigallocatechin Gallate in Comparison to Its Major Oxidation Products and Their Antioxidant Capacity In Vitro

Alfke

Esselen

2022

Antioxidants

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Depletion of reactive oxygen species and reduction of oxidative stress have been identified as key parameters in the prevention of cellular aging. In previous in vitro studies, the tea catechin epigallocatechin gallate (EGCG) was found to have both pro- and antioxidant properties, disregarding the low stability under cell culture conditions. Besides hydrogen peroxide, theasinensin dimers amongst other oxidation products are formed. Exact quantities, cellular uptake and antioxidant capacities of these dimeric oxidation products remain unknown. Via high-performance liquid chromatography (HPLC) coupled with tandem mass spectrometry (MS/MS), formation kinetics and cellular uptake of EGCG and its major oxidation products are quantified. The antioxidant capacity is determined on a cellular level using a modified dichlorofluorescein (DCF) approach. As a first result, oxidation product quantities of up to 21 µM each are measured after incubation of 50 µM EGCG. While EGCG is taken up equimolarly, its major oxidation products are accumulated in hepatocarcinoma HepG2 cells at millimolar concentrations, especially theasinensin A (TSA). Lastly, the oxidation products show higher antioxidant properties than the monomer EGCG. In correlation with cellular uptake, TSA displays the highest capacity of all tested analytes. The findings reveal the strong influence of EGCG oxidation products on its bioactivity in vitro.

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Intracellular uptake of EGCG-loaded deformable controlled release liposomes for skin cancer

Abstract: Caucasian population groups have a higher propensity to develop skin cancer, and associated '" clinical interventions often present substantial financial burden on healthcare services.

Cited by 34 publications

References 88 publications

Sustained Release of a Streptococcus pneumoniae Endolysin from Liposomes for Potential Otitis Media Treatment

Sustained Release of a Streptococcus pneumoniae Endolysin from Liposomes for Potential Otitis Media Treatment

Interplay of Nanoparticle Properties during Endocytosis

Cellular Uptake of Epigallocatechin Gallate in Comparison to Its Major Oxidation Products and Their Antioxidant Capacity In Vitro

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