Intracellular viability in human non-polarized respiratory epithelial 16 HBE 14o- cells by group B Streptococcus serotype III clinical isolates presenting 162-kb and 183-kb virulence markers

José, Adriana Sá de São; Miyazaki, Neide H.T.; Hirata, Raphael; Mattos‐Guaraldi, Ana Luíza; Nagao, Prescilla Emy

doi:10.3892/ijmm.17.3.533

Cited by 3 publications

References 18 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

Metallopeptidases produced by group B Streptococcus: Influence of proteolytic inhibitors on growth and on interaction with human cell lineages

Soares¹,

Silva²,

Santos³

et al. 2008

Int J Mol Med

Self Cite

View full text Add to dashboard Cite

Abstract. Group B Streptococcus (GBS) is a major etiologic agent of neonatal bacterial infections and is the most common cause of sepsis and pneumonia in newborns. Surface and secreted molecules of GBS are often essential virulence factors which are involved in the adherence of the bacteria to host cells or are required to suppress the defense mechanisms of hosts. We analyzed the peptidase profiles of GBS by detection of proteolytic activities on SDS-PAGE containing copolymerized gelatin as substrate. Based on the inhibition by o-phenathroline and EGTA, three distinct peptidases of 220, 200 and 180 kDa were identified in the culture medium, besides one major cell-associated proteolytic activity, a 200-kDa metallopeptidase, suggesting that all were zincmetallopeptidases. GBS culture supernatants, rich in metallotype peptidases, also cleaved fibronectin, laminin, type IV collagen, fibrinogen and albumin. Cleavage of the host extracellular matrix by GBS may be a relevant factor in the process of bacterial dissemination and/or invasion. Notably, metallopeptidase inhibitors strongly blocked GBS growth as well as its interaction with human cell lineages. Understanding the contribution of peptidases to the pathogenesis of GBS disease may broaden our perception of how this significant pathogen causes severe infections in newborn infants.

show abstract

Metallopeptidases produced by group B Streptococcus: Influence of proteolytic inhibitors on growth and on interaction with human cell lineages

Soares¹,

Silva²,

Santos³

et al. 2008

Int J Mol Med

Self Cite

View full text Add to dashboard Cite

show abstract

Effect of chelating agents on the growth, surface polypeptide synthesis and interaction of Streptococcus agalactiae with human epithelial cells

Santos

Costa²,

Santos³

et al. 2008

Mol Med Rep

View full text Add to dashboard Cite

Abstract. In the present study, the influence of the chelating agents of ethylenediaminetetraacetic acid (EDTA), ethylene glycol-bis (ß-aminoethyl ether) (EGTA) and 1,10-phenanthroline (PHEN) on growth rate, cytoadherence ability and surface protein expression was examined in a clinical strain of Streptococcus agalactiae isolated from a blood sample. EDTA and EGTA at 10 mM did not inhibit the growth of S. agalactiae, while PHEN significantly arrested bacterial proliferation in a concentration range of 10-0.01 mM. The in vitro interaction between S. agalactiae and A549 cells was a time-dependent process; adherence and bacterial intracellular viability were more pronounced after 3 h of contact. The pre-treatment of bacterial cells with EDTA adversely influenced the adhesive properties of S. agalactiae to A549 cells after 2 and 3 h, whereas EGTA only blocked this process after 3 h. Viable intracellular bacteria were just detected after 3 h of interaction, and EDTA and EGTA inhibited intracellular viability in a similar fashion. Conversely, PHEN inhibited neither the adherence nor the intracellular viability of the microorganism. Furthermore, EDTA robustly suppressed surface polypeptide synthesis, suggesting a decline in the possible bacterial ligands responsible for S. agalactiae adhesive properties.

show abstract

Mutant Group B Streptococcus surface expressed Phosphoglycerate kinase (PGK) with reduced plasminogen binding

Siddiqua¹

2014

View full text Add to dashboard Cite

Intracellular viability in human non-polarized respiratory epithelial 16 HBE 14o- cells by group B Streptococcus serotype III clinical isolates presenting 162-kb and 183-kb virulence markers

Cited by 3 publications

References 18 publications

Metallopeptidases produced by group B Streptococcus: Influence of proteolytic inhibitors on growth and on interaction with human cell lineages

Metallopeptidases produced by group B Streptococcus: Influence of proteolytic inhibitors on growth and on interaction with human cell lineages

Effect of chelating agents on the growth, surface polypeptide synthesis and interaction of Streptococcus agalactiae with human epithelial cells

Mutant Group B Streptococcus surface expressed Phosphoglycerate kinase (PGK) with reduced plasminogen binding

Contact Info

Product

Resources

About