Intracoronary artery transplantation of cardiomyoblast-like cells from human adipose tissue-derived multi-lineage progenitor cells improve left ventricular dysfunction and survival in a swine model of chronic myocardial infarction

Okura, Hanayuki; Saga, Ayami; Soeda, Mayumi; Miyagawa, Shigeru; Sawa, Yoshiki; Daimon, Takashi; Ichinose, Akihiro; Matsuyama, Akifumi

doi:10.1016/j.bbrc.2012.08.004

Cited by 17 publications

(14 citation statements)

References 27 publications

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“…In a series of studies, we have characterized hADMPCs as a novel and minor cell population in human adipose tissues and reported that these cells have stem cell properties including their ability to be reprogrammed in situ into cardiomyocytes in rats with MI and into hepatocyte-like cells in the rabbit hepatic parenchyma [9][10][11][12]. Based on their advantages, hADMPCs are promising source of cells for cell therapy.…”

Section: Discussionmentioning

confidence: 99%

“…Adipose tissue samples were resected from 5 human subjects Isolation of hADMPCs and spermine-treatment hADMPCs were prepared as described in detail previously [9][10][11][12]14,15]. Briefly, the resected excess adipose tissue was minced and then digested at 37°C for 1 h in Hank's balanced salt solution (HBSS, GIBCO Invitrogen, Grand Island, NY) with Liberase (Roche Diagnostics, Germany).…”

Section: Adipose Tissuementioning

confidence: 99%

“…A chronic MI swine model was prepared as described in detail previously [12]. The protocol was shown in Figure 1A.…”

Section: Animal Model Of MI and Cell Transplantationmentioning

confidence: 99%

“…In a series of publications, our group reported that adipose tissue-derived multi-lineage progenitor cells (ADMPC), which met the criteria of mesenchymal stem cells [9], could be reprogrammed into hepatocytes both in vitro and in vivo [10,11]. However, human ADMPCs (hADMPCs) per se could not survive in cardiac parenchyme longer duration after transplantation and not differentiate into cardiomyocytes in vivo [12].…”

Section: Introductionmentioning

confidence: 99%

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Spermine Treated-Adipose Tissue-Derived Multi-Lineage Progenitor Cells Improve Left Ventricular Dysfunction in a Swine Model of Chronic Myocardial Infarction

Okura¹,

Morita²

2016

J Stem Cell Res Ther

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Section: Discussionmentioning

confidence: 99%

Section: Adipose Tissuementioning

confidence: 99%

“…A chronic MI swine model was prepared as described in detail previously [12]. The protocol was shown in Figure 1A.…”

Section: Animal Model Of MI and Cell Transplantationmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Spermine Treated-Adipose Tissue-Derived Multi-Lineage Progenitor Cells Improve Left Ventricular Dysfunction in a Swine Model of Chronic Myocardial Infarction

Okura¹,

Morita²

2016

J Stem Cell Res Ther

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“…Recent advances in regenerative medicine have promised us the innovative therapeutic approaches for intractable diseases [1][2][3][4]. To move forward regenerative medicine into clinical application, some issues are here.…”

Section: Introductionmentioning

confidence: 99%

Regulatory Aspect of Pre-clinical Studies for Regenerative Medicine

Okura¹,

Matsuyama²

2016

Transl Med (sunnyvale)

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Regenerative cell therapy is a discipline in youth, with only limited experience in clinical practice. Once manufacturing protocols of cells have been established in vitro studies, the manufactured cells were assigned as candidates for cell-based medicinal products. To judge whether the cell-based medicinal products manufactured according to the established protocols should be valuable for further development for clinical practice, pre-clinical studies shall be conducted. In construction of a pre-clinical study package for cell-based medicinal products, the preclinical study package used for chemical compounds will help a lot. The requirements and contents of the pre-clinical studies are classified into toxicity studies (general toxicity studies and special toxicity studies), pharmacological studies (primary pharmacodynamics studies and safety pharmacological studies), pharmacokinetic studies, formulation studies, and others. The pre-clinical studies for the cell-based medicinal products are conducted to evaluate and qualify the efficacy and safety of the candidates. No wonder there is an argument about the selection of assessment parameters in toxicity, pharmacological and pharmacokinetics studies for cell-based medicinal product, because it is well-known that the cell-based medicinal products are different in properties from chemical compounds. Besides parameters used in these studies, the assessment issues may be an assumable minimum consensus, and others should be added on a case-by-case basis, depending on the cell manufacturing processes. In anticipation of the approval as the cell-based medicinal products, the possible requirements should be discussed here in the development process of such products from the standpoint of regulatory science, especially focusing on those in pre-clinical studies.

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A canine acute coronary occlusion model using thrombus implantation by percutaneous coronary intervention

Ma,

Yang,

Zhang

et al. 2023

iRADIOLOGY

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ObjectiveTo establish an acute canine coronary artery occlusion model by implanting a mixed thrombus prepared in vitro into the coronary artery by percutaneous coronary intervention (PCI).MethodsThis study included 14 healthy Beagle dogs aged 10–13 months. First, 5 ml of canine venous blood was drawn to prepare the coronary artery thrombus in vitro. The thrombus was then implanted into the canine coronary artery by PCI to establish an animal model of acute coronary artery occlusion. Dogs were randomly divided into two experimental groups: group A, in which dogs were assessed for 2 h after coronary occlusion, and group B, in which dogs were assessed for 4 h after surgery. Baseline and postoperative assessment included cardiac troponin T (cTnT) levels, electrocardiography (ECG), plain and contrast echocardiography, and large‐scale digital subtraction angiography (DSA).ResultsCanine venous blood was successfully used to prepare a mixed thrombus with reticular fibrin structure and activated platelets in vitro. After the mixed thrombus was implanted into the coronary artery, large‐scale DSA showed no contrast medium perfusion, confirming complete occlusion of the coronary artery. After the coronary artery occlusion, dogs in both groups showed ST segment elevation >0.5 MV, a 0.9 μg/L increase in CTnT (p < 0.001), and increased EDV and ESV. Dogs also showed decreased left ventricular EF and FS and decreased MBF in the infarcted area (p < 0.05).ConclusionsAn experimental model of acute coronary artery occlusion in dogs was successfully established by implanting a mixed thrombus developed in vitro into the coronary artery using minimally invasive PCI.

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Intracoronary artery transplantation of cardiomyoblast-like cells from human adipose tissue-derived multi-lineage progenitor cells improve left ventricular dysfunction and survival in a swine model of chronic myocardial infarction

Cited by 17 publications

References 27 publications

Spermine Treated-Adipose Tissue-Derived Multi-Lineage Progenitor Cells Improve Left Ventricular Dysfunction in a Swine Model of Chronic Myocardial Infarction

Spermine Treated-Adipose Tissue-Derived Multi-Lineage Progenitor Cells Improve Left Ventricular Dysfunction in a Swine Model of Chronic Myocardial Infarction

Regulatory Aspect of Pre-clinical Studies for Regenerative Medicine

A canine acute coronary occlusion model using thrombus implantation by percutaneous coronary intervention

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