Intracytoplasmic Re-localization of miRISC Complexes

Akgül, Bünyamin; Erdoğan, İpek

doi:10.3389/fgene.2018.00403

Cited by 22 publications

(14 citation statements)

References 81 publications

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“…The miRNA-loaded AGO associates with other cofactors, including GW182 (also known as TNRC6A), and constitutes the effector complex called the RNA-induced silencing complex (RISC) [2]. The miRISC (miRNA-induced silencing complex) induces the decay of mRNA and translational suppression through the interaction with the complementary sequences in the 3 -untranslated region (3 -UTR) of target gene mRNA [3][4][5]. The miRNAs target a majority of mRNAs, enabling them to have important regulatory roles in diverse physiological and developmental processes [6].…”

Section: Introductionmentioning

confidence: 99%

Regulatory Mechanism of MicroRNA Expression in Cancer

Syeda

Langden

Munkhzul

et al. 2020

IJMS

702

415

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Altered gene expression is the primary molecular mechanism responsible for the pathological processes of human diseases, including cancer. MicroRNAs (miRNAs) are virtually involved at the post-transcriptional level and bind to 3′ UTR of their target messenger RNA (mRNA) to suppress expression. Dysfunction of miRNAs disturbs expression of oncogenic or tumor-suppressive target genes, which is implicated in cancer pathogenesis. As such, a large number of miRNAs have been found to be downregulated or upregulated in human cancers and to function as oncomiRs or oncosuppressor miRs. Notably, the molecular mechanism underlying the dysregulation of miRNA expression in cancer has been recently uncovered. The genetic deletion or amplification and epigenetic methylation of miRNA genomic loci and the transcription factor-mediated regulation of primary miRNA often alter the landscape of miRNA expression in cancer. Dysregulation of the multiple processing steps in mature miRNA biogenesis can also cause alterations in miRNA expression in cancer. Detailed knowledge of the regulatory mechanism of miRNAs in cancer is essential for understanding its physiological role and the implications of cancer-associated dysfunction and dysregulation. In this review, we elucidate how miRNA expression is deregulated in cancer, paying particular attention to the cancer-associated transcriptional and post-transcriptional factors that execute miRNA programs.

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Section: Introductionmentioning

confidence: 99%

Regulatory Mechanism of MicroRNA Expression in Cancer

Syeda

Langden

Munkhzul

et al. 2020

IJMS

702

415

View full text Add to dashboard Cite

show abstract

“…38 Regulates gene expression posttranscriptionally, primarily by targeting specific mRNAs for translational repression and/or mRNA degradation. [39][40][41] It can be involved in a variety of life processes, including cell proliferation, differentiation, apoptosis and migration, 42 and is a central player in many physiological and pathological processes. 38 miRNAs can also directly regulate the EMT process of pulmonary epithelial cells.…”

Section: Discussionmentioning

confidence: 99%

LncRNA MRAK052509 competitively adsorbs miR‐204‐3p to regulate silica dust‐induced EMT process

Xuan,

Zi‐ming,

Xue‐yan

et al. 2024

Environmental Toxicology

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Silicosis is a systemic disease caused by long‐term inhalation of free SiO2 and retention in the lungs. At present, it is still the most important occupational health hazard disease in the world. Existing studies have shown that non‐coding RNA can also participate in complex fibrosis regulatory networks. However, its role in regulating silicotic fibrosis is still unclear. In this study, we constructed a NR8383/RLE‐6TN co‐culture system to simulate the pathogenesis of silicosis in vitro. Design of miR‐204‐3p mimics and inhibitors to overexpress or downregulate miR‐204‐3p in RLE‐6TN cells. Design of short hairpin RNA (sh‐RNA) to downregulate MRAK052509 in RLE‐6TN cells. The regulatory mechanism of miR‐204‐3p and LncRNA MRAK052509 on EMT process was studied by Quantitative real‐time PCR, Western blotting, Immunofluorescence and Cell scratch test. The results revealed that miR‐204‐3p affects the occurrence of silica dust‐induced cellular EMT process mainly through regulating TGF‐βRΙ, a key molecule of TGF‐β signaling pathway. In contrast, Lnc MRAK052509 promotes the EMT process in epithelial cells by competitively adsorbing miR‐204‐3p and reducing its inhibitory effect on the target gene TGF‐βRΙ, which may influence the development of silicosis fibrosis. This study perfects the targeted regulation relationship between LncRNA MRAK052509, miR‐204‐3p and TGF‐βRΙ, and may provide a new strategy for the study of the pathogenesis and treatment of silicosis.

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“…The pre-miRNA is transported from the nucleus into the cytoplasm by exportin 5 and then processed by Dicer/TRBP into an miRNA duplex [75][76][77]. After the formation of a duplex complex with the RNA-induced silencing complex (RISC), mature microRNAs are packed into exosomes in different manners: I) the miRISC-related pathway, II) miRNA motif-sumoylated heterogeneous nuclear ribonucleoproteins (hnRNPs)-and the synaptotagmin-binding cytoplasmic-RNA-interacting-protein (SYNCRIP)-dependent pathway, which recognizes the short sequence motifs GGAG and GCUG in the 3 -miRNA sequence, and III) the ceramide-dependent pathway [78][79][80][81][82][83][84]. In addition, RNA-binding protein (heterogeneous nuclear ribonucleoprotein A2B1: hnRNPA2B1) and Y-box protein are involved in the sorting of miRNAs in the EVs [85,86].…”

Section: Proteins Peptides Lipid and Nucleic Acids In Exosomesmentioning

confidence: 99%

Extracellular Vesicles as Novel Drug-Delivery Systems through Intracellular Communications

Matsuzaka

Yashiro

2022

Membranes

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Since it has been reported that extracellular vesicles (EVs) carry cargo using cell-to-cell comminication according to various in vivo situations, they are exprected to be applied as new drug-delivery systems (DDSs). In addition, non-coding RNAs, such as microRNAs (miRNAs), have attracted much attention as potential biomarkers in the encapsulated extracellular-vesicle (EV) form. EVs are bilayer-based lipids with heterogeneous populations of varying sizes and compositions. The EV-mediated transport of contents, which includes proteins, lipids, and nucleic acids, has attracted attention as a DDS through intracellular communication. Many reports have been made on the development of methods for introducing molecules into EVs and efficient methods for introducing them into target vesicles. In this review, we outline the possible molecular mechanisms by which miRNAs in exosomes participate in the post-transcriptional regulation of signaling pathways via cell–cell communication as novel DDSs, especially small EVs.

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Intracytoplasmic Re-localization of miRISC Complexes

Cited by 22 publications

References 81 publications

Regulatory Mechanism of MicroRNA Expression in Cancer

Regulatory Mechanism of MicroRNA Expression in Cancer

LncRNA MRAK052509 competitively adsorbs miR‐204‐3p to regulate silica dust‐induced EMT process

Extracellular Vesicles as Novel Drug-Delivery Systems through Intracellular Communications

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