Intraepitheliale Neoplasien (PanIN) und intraduktale papillär-muzinöse Neoplasien (IPMN) des Pankreas als Vorläufer des Pankreaskarzinoms

Ott, Claudia; Heinmöller, Ernst; Gäumann, Andreas; Schölmerich, Jürgen; Klebl, Frank

doi:10.1007/s00063-007-1013-8

Cited by 10 publications

(7 citation statements)

References 58 publications

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“…As well, PanIN was disregarded due to the size of the lesion and the lack of small epithelial papillae [15]. In SPN a combination of solid and pseudopapillary growth pattern is frequently present but these tumors are usually immunoreactive for CD10, vimentin, chromogranin A and nuclear β-catenin [16,17].…”

Section: Discussionmentioning

confidence: 99%

Case report: intraductal tubulopapillary neoplasm of the pancreas with unique clear cell phenotype

et al. 2014

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Intraductal tubulopapillary neoplasms of the pancreas are very rare tumors characterized by intraductal tubulopapillary growth, ductal differentiation, scant intracellular mucin production and cellular dysplasia. Here, we report the first case of an intraductal tubulopapillary neoplasm of the pancreas with clear cell morphology. The tumor was detected during the diagnostic work-up of acute pancreatitis in a 43- year old female. Histological examination revealed a tumor with the typical architecture of an intraductal tubulopapillary neoplasm of the pancreas with tumor cells showing abundant clear cytoplasm and Di-PAS negativity. Immunohistochemistry revealed positivity for Pan-CK, CK7, CK8/18, MUC1, MUC6, carbonic anhydrase IX, CD10, EMA, β-catenin and e-cadherin. Sanger sequencing did not detect mutations for β-catenin, BRAF, KRAS, PIK3CA and GNAS. Altogether, histology, immunohistochemical expression profile (MUC1+, MUC6+, MUC2-, MUC5AC-, thrypsin-, chymotrypsin-, CDX2-) and sequencing results led to the diagnosis of intraductal tubulopapillary neoplasm. However, the neoplasm consisted of cells showing abundant clear cytoplasm, a morphological pattern not being described so far in the current classification of pancreatic intraductal neoplasms. Potential differential diagnosis and the molecular basis of clear cell morphology are discussed. In conclusion, we consider this tumor as intraductal tubulopapillary neoplasm of the pancreas with unique clear cell phenotype. After surgery and without adjuvant therapy, the patient’s clinical course has been uneventful for over two years now.Virtual slidesThe virtual slide(s) for this article can be found here: http://www.diagnosticpathology.diagnomx.eu/vs/1051828790117127

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Section: Discussionmentioning

confidence: 99%

Case report: intraductal tubulopapillary neoplasm of the pancreas with unique clear cell phenotype

et al. 2014

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“…Then, either small cell aggregates of approximately 1 × 10 5 cells [ 70 ] or a cell suspension [ 71 ] are injected. Orthotopically transplanted organoids in mice have been reported to create preinvasive lesions that resemble pancreatic intraepithelial neoplasia (PanIN) [ 48 , 76 ], discussed as a precursor of PDAC [ 122 ]. Additionally, after forming PanIN-similar lesions, the organoids progress into invasive PDAC [ 48 ].…”

Section: Translation Of 3d Cell Culture Models From In Vitro To Inmentioning

confidence: 99%

Three-Dimensional Cell Culture Systems in Radiopharmaceutical Cancer Research

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Seifert

Ullrich

et al. 2020

Cancers

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In preclinical cancer research, three-dimensional (3D) cell culture systems such as multicellular spheroids and organoids are becoming increasingly important. They provide valuable information before studies on animal models begin and, in some cases, are even suitable for reducing or replacing animal experiments. Furthermore, they recapitulate microtumors, metastases, and the tumor microenvironment much better than monolayer culture systems could. Three-dimensional models show higher structural complexity and diverse cell interactions while reflecting (patho)physiological phenomena such as oxygen and nutrient gradients in the course of their growth or development. These interactions and properties are of great importance for understanding the pathophysiological importance of stromal cells and the extracellular matrix for tumor progression, treatment response, or resistance mechanisms of solid tumors. Special emphasis is placed on co-cultivation with tumor-associated cells, which further increases the predictive value of 3D models, e.g., for drug development. The aim of this overview is to shed light on selected 3D models and their advantages and disadvantages, especially from the radiopharmacist’s point of view with focus on the suitability of 3D models for the radiopharmacological characterization of novel radiotracers and radiotherapeutics. Special attention is paid to pancreatic ductal adenocarcinoma (PDAC) as a predestined target for the development of new radionuclide-based theranostics.

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“…High-grade PanINs stellen auf zellulärer Ebene aufgrund u. a. aufgehobener Zellpo-larität, gehäuften Mitosen und Kernunregelmäßigkeiten Karzinome dar, die die Basalmembran nicht überschreiten (entsprechend dem Konzept des Carcinoma in situ) [2]. Sie lassen sich oft in Pankreaskarzinom-Resektaten nachweisen.…”

Section: Vorläuferläsionenunclassified