Intragastric administration of <i>Mycobacterium vaccae</i> inhibits severe pulmonary allergic inflammation in a mouse model

Hunt, Jon; Martinelli, Roberta; Adams, Victoria; Rook, G.A.W.; Brunet, Laura Rosa

doi:10.1111/j.1365-2222.2005.02239.x

Cited by 57 publications

(42 citation statements)

References 29 publications

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“…There have been a few reports about the 8-isoprostane level of the EBC in moderate asthma patients [11, 26] or the 8-isoprostane level of EBC in mild asthma patients. The level of 8-isoprostane in mild asthma was reported to range from 10 to 20 pg/ml [28, 29]. We could not exclude the effect of ICS treatment on the level of 8-isoprostane in the present study compared with previous reports, and it will be necessary to measure 8-isoprostane in EBC from more moderate asthma patients.…”

Section: Discussioncontrasting

confidence: 50%

Proton Pump Inhibitor Improves Breath Marker in Moderate Asthma with Gastroesophageal Reflux Disease

Shimizu

Dobashi²,

Zhao³

et al. 2007

Respiration

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Background: Gastroesophageal reflux disease (GERD) influences the symptoms of asthma with acid and oxidative stress. Objectives: The purpose of this study was to determine the usefulness of measurement of the acid stress marker pH and the oxidative stress marker 8-isoprostane by exhaled breath condensate in proton pump inhibitor (PPI) therapy effect on moderate asthma patients with GERD. Methods: The pH and the concentration of 8-isoprostane were measured in the exhaled breath condensate of patients with moderate asthma (n = 36) and healthy subjects (n = 26). Two months of PPI therapy (lansoprazole at 30 mg/day) were done in the asthma patients with (n = 13) or without (n = 13) GERD according to a questionnaire for the diagnosis of reflux disease, and exhaled markers were measured. Results: The pH was lower (7.3 ± 0.3) and the 8-isoprostane level was higher (27.7 ± 2.3) in the asthma patients than in the healthy control subjects (pH 7.5 ± 0.2 and 8-isoprostane 6.6 ± 1.2). Two months of PPI therapy improved the pH (from 7.2 ± 0.1 to 7.3 ± 0.1) and the 8-isoprostane concentration (from 32.7 ± 3.4 to 19.2 ± 3.4) in the asthma patients with GERD, along with improvement of GERD symptoms. However, these markers did not change in the asthma patients without GERD. Conclusions: Measurement of the pH and 8-isoprostane level of exhaled breath condensate may be useful to evaluate the influence of GERD on asthma, as well as to determine the timing of intermittent PPI therapy.

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Section: Discussioncontrasting

confidence: 50%

Proton Pump Inhibitor Improves Breath Marker in Moderate Asthma with Gastroesophageal Reflux Disease

Shimizu

Dobashi²,

Zhao³

et al. 2007

Respiration

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“…Following two further immunizations with allergen, mice were challenged by the intratracheal route 40 days after the intragastric dose of M. vaccae. Interestingly, M. vaccae was as effective by this route as it was by the subcutaneous route, and at precisely the same doses (Hunt et al, 2005). Since much of an oral dose must be lost, this implies that the oral route is particularly efficient, in agreement with the observations that mycobacteria are easily picked up by M cells on Peyer's patches (Fujimura, 1986) and that the gut may be a major site of induction of RegT (Powrie et al, 2003).…”

Section: Vaccae By the Oral Routesupporting

confidence: 53%

Mycobacteria and allergies

2007

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“…It was also shown that intragastric administration of M. vaccae inhibited pulmonary allergic inflammation in murine models with increased production of IL-10 and IFN-γ [35]. In the agreement with these findings, we showed that OVA induced IL-10 levels tended to be higher in the M. vaccae/OVA group compared to other groups.…”

Section: Discussionsupporting

confidence: 81%

“…Hunt et al showed that a single intragastric administration of M. vaccae in mice induced a transient increase in the production of IL-10 and IFN-γ by mesenteric lymph nodes and splenocytes [35]. In addition Zuany-Amorim et al demonstrated that killed M. vaccae gives rise to allergen-specific CD4 + CD45RB Lo regulatory T cells, which were able to protect from airway inflammation in OVA-sensitized mice [40].…”

Section: Discussionmentioning

confidence: 99%

<i>Mycobacterium vaccae</i> Immunization to Pregnant BALB/c Mice Ameliorated Lung Histopathology and Bone Marrow Eosinophila in Ovalbumin Sensitized Offsprings

Akkoç

Özdemir²,

Yazi³

et al. 2014

OJI

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Context: One of the treatment strategies for atopic diseases is to skew immune response away from Th2 dominance by using Mycobacterial strains. Objective: We wanted to find out whether M. vaccae administration to pregnant mice had any preventive effect on the offsprings in the development of a murine asthma model. Materials and Methods: Pregnant BALB/c mice were divided into two groups; first group received heat-killed M. vaccae subcutaneously on 12 th day of pregnancy and the latter group received PBS. After delivery, newborn mice of each group were further divided into two subgroups as M. vaccae/Ovalbumin (OVA), M. vaccae/control, PBS/OVA and PBS/ control. To establish experimental murine asthma model, mice were intraperitoneally sensitized and challenged intratracheally with Ovalbumin. We analysed airway histopathology, bone marrow eosinophil progenitors and splenic cell cytokine profiles of the offsprings. Results: Comparison of offsprings in M. vaccae/OVA group were not different than PBS controls with respect to thicknesses of airway epithelium, basement membrane, subepithelial smooth muscles and number of hyperplasic goblet cells as well as bronchial associated lymphoid tissue density and eosinophil progenitors in the bone marrow. Comparison of M. vaccae/OVA group to asthma model revealed significant differences and lower levels of OVA-induced IL-5. Conclusions: We propose that immunization of pregnant BALB/c with M. vaccae could prevent histopathological alterations in the airways related to the asthma model and down-regulates IL-5 secretion from splenocytes of offsprings.

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Intragastric administration of Mycobacterium vaccae inhibits severe pulmonary allergic inflammation in a mouse model

Abstract: This is the first report to suggest that heat-killed mycobacteria can down-regulate symptoms of allergic inflammation by the intragastric route. These data suggest an alternative route of treatment with M. vaccae for patients with allergic conditions.

Cited by 57 publications

References 29 publications

Proton Pump Inhibitor Improves Breath Marker in Moderate Asthma with Gastroesophageal Reflux Disease

Proton Pump Inhibitor Improves Breath Marker in Moderate Asthma with Gastroesophageal Reflux Disease

Mycobacteria and allergies

<i>Mycobacterium vaccae</i> Immunization to Pregnant BALB/c Mice Ameliorated Lung Histopathology and Bone Marrow Eosinophila in Ovalbumin Sensitized Offsprings

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Intragastric administration of Mycobacterium vaccae inhibits severe pulmonary allergic inflammation in a mouse model

Abstract: This is the first report to suggest that heat-killed mycobacteria can down-regulate symptoms of allergic inflammation by the intragastric route. These data suggest an alternative route of treatment with M. vaccae for patients with allergic conditions.

Cited by 57 publications

References 29 publications

Proton Pump Inhibitor Improves Breath Marker in Moderate Asthma with Gastroesophageal Reflux Disease

Proton Pump Inhibitor Improves Breath Marker in Moderate Asthma with Gastroesophageal Reflux Disease

Mycobacteria and allergies

&lt;i&gt;Mycobacterium vaccae&lt;/i&gt; Immunization to Pregnant BALB/c Mice Ameliorated Lung Histopathology and Bone Marrow Eosinophila in Ovalbumin Sensitized Offsprings

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<i>Mycobacterium vaccae</i> Immunization to Pregnant BALB/c Mice Ameliorated Lung Histopathology and Bone Marrow Eosinophila in Ovalbumin Sensitized Offsprings