Intragastric administration of Lactobacillus casei expressing transmissible gastroentritis coronavirus spike glycoprotein induced specific antibody production

Ho, Phui San; Kwang, Jimmy; Lee, Y.K.

doi:10.1016/j.vaccine.2004.09.015

Cited by 64 publications

(60 citation statements)

References 32 publications

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“…Briefly, an aliquot of frozen heat-killed L. casei was thawed to room temperature and each mouse received 1 dose of 100 µl containing 10 9 heat-killed L. casei or 100 µl of NaHCO 3 buffer per day. The mice were dosed for 3 consecutive days in a week [18] for 5 weeks, starting at day 35. At days 55 and 62, mice were challenged with 2 subcutaneous injections of Der p 2 (50 µg/mouse) followed by 2 subsequent aerosol challenges with Der p 2 allergen (1 mg/10 ml PBS).…”

Section: Methodsmentioning

confidence: 99%

The Effects of Heat-Killed Wild-Type <i>Lactobacillus casei </i>Shirota on Allergic Immune Responses in an Allergy Mouse Model

Lim¹,

Li²,

Huang³

et al. 2008

Int Arch Allergy Immunol

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Background: Probiotics are used as a management strategy for allergic diseases, but their effects on allergic responses in sensitized allergic individuals remain unclear. This study explored the immunomodulatory effects of probiotics on allergen-specific allergic reactions in an allergy mouse model. Methods: C57BL/6 mice were presensitized by epicutaneous patching with recombinant Der p 2, and were subsequently administered orally with either heat-killed wild-type Lactobacillus casei or NaHCO₃ buffer for 5 weeks (n = 6 per group). All mice then received 2 subcutaneous immunizations of Der p 2 to mimic allergen immunotherapy, followed by aerosol challenge with Der p 2 a week later. Results: The Der p 2-sensitized mice fed L. casei showed significantly lower Der p 2-specific IgE and IgG1 after subcutaneous immunizations and airway challenge with Der p 2 compared to the control, unfed group. Splenic T cells of mice fed L. casei showed suppression of Th-2 (IL-5, IL-13, IL-10 and IL-4) and proinflammatory (TNF-α, IFN-γ) cytokines. Following airway allergen challenge, the mice fed L. casei showed histological evidence of attenuation of lung inflammation, as well as reductions in the total cell count and Th2 and proinflammatory cytokines in bronchoalveolar lavage fluid, compared to controls. Taken together, these results suggest that the oral administration of heat-killed L. casei could effectively downregulate the pre-existing Th-2 allergic responses and pulmonary inflammatory responses upon subcutaneous and airway allergen challenge. Conclusions:L. casei has intrinsic adjuvant and immunomodulatory properties that could potentially be exploited for secondary prevention or treatment of allergic respiratory diseases.

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Section: Methodsmentioning

confidence: 99%

The Effects of Heat-Killed Wild-Type <i>Lactobacillus casei </i>Shirota on Allergic Immune Responses in an Allergy Mouse Model

Lim¹,

Li²,

Huang³

et al. 2008

Int Arch Allergy Immunol

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“…Bacterium was labeled with fluorescence probe as described previously [28]. In brief, Log-phase culture of Lc was harvested, washed twice with sterile phosphate-buffered saline (PBS) and adjusted to a concentration of 10 10 CFU ml À1 prior to labeling with 50 mM five-(and 6-) carboxyfluorescein diacetate succinimidyl ester (cFDA-SE) at 37 C for 20 min.…”

Section: Adhesion On Mucosal Surfacementioning

confidence: 99%

“…Preparation and electrotransformation of competent L. casei were performed as described previously [28]. Briefly, a stationary phase (16e18 h) culture of recipient L. casei was inoculated (1/50 inoculum) (v/v) into 100 ml MRS broth and incubated at 37 C without shaking.…”

Section: Recombinant Bacteria Proceduresmentioning

confidence: 99%

Immunogenicity of Lactobacillus-expressing VP2 and VP3 of the infectious pancreatic necrosis virus (IPNV) in rainbow trout

Liu

Zhao

et al. 2012

Fish & Shellfish Immunology

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“…One promising approach relies on the use of live bacterial vehicles (21). The potentiality of recombinant lactic acid bacteria to deliver heterologous antigens to the mucosal immune system has been investigated during the last decade (10,11,19,28,30,31,37). This approach offers a number of advantages (such as noninvasiveness and the possibility of eliciting both mucosal and systemic immune responses) over the traditional parenteral vaccination.…”

mentioning

confidence: 99%

“…Lactobacilli represent an original alternative to the use of attenuated pathogenic bacterial carriers, such as Salmonella, Vibrio, and Mycobacterium (6,16,34). Lactobacillus strains have been used as hosts to express many bacterial and viral antigens and have been proven to elicit immune responses after being used for inoculation by oral administration ( 10,11,19,31,37), which makes them attractive candidates from a pharmaceutical standpoint as agents for the delivery of antigens to the mucosa in particular vaccines. Furthermore, lactobacilli could survive transit through the upper gastrointestinal tract and colonize the intestinal tracts (1,17).…”

mentioning

confidence: 99%

Induction of Immune Responses in Mice after Intragastric Administration of Lactobacillus casei Producing Porcine Parvovirus VP2 Protein

2007

Appl Environ Microbiol

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Lactobacillus casei ATCC 393 was selected as an antigen delivery vehicle for mucosal immunization against porcine parvovirus (PPV) infection. A 64-kDa fragment of PPV major protective antigen VP2 protein was used as the parvovirus antigen model. A recombinant Lactobacillus expressing VP2 protein was constructed with plasmid pPG611.1, where expression and localization of the VP2 protein from recombinant Lc393-rPPV-VP2 was detected via sodium dodecyl sulfate-polyacrylamide gel electrophoresis, Western blotting, and immunofluorescence. Both local mucosal and systemic immune responses against PPV were induced in BALB/c mice immunized orally with the recombinant Lactobacillus expressing VP2 protein. The induced antibodies demonstrated neutralizing effects on PPV infection. These data indicated that the use of recombinant lactobacilli could be a valuable strategy for future vaccine development of PPV.Porcine parvovirus (PPV), characterized as a member of the autonomous parvoviruses, is a major cause of reproductive failure in swine, resulting in early embryonic death, fetal death, stillbirths, and delayed return to estrus (3,12,13,22), and the virus causes serious losses for pig producers. The molecular features of PPV are similar to those of other autonomous parvoviruses. PPV is a small, nonenveloped virus, and the virion contains a 5-kb, linear, minus-polarity, single-stranded DNA genome (25), which is encapsidated within a simple icosahedron protein coat composed of three structural polypeptides (VP1, VP2, and VP3, with masses of 84, 64, and 60 kDa, respectively) (24). The VP2 protein encompasses major antigenic domains of PPV and could induce PPV-neutralizing antibodies for the neutralization of PPV infection (26,32,38). Therefore, the VP2 protein plays a major role in PPV diagnosis and immune prophylaxis.The mucosa tissues are particularly important for protection against diseases, such as those caused by viral, bacterial, and parasitic pathogens which invade the mucosal system (18, 39). Vaccines administered by parenteral routes generally fail to stimulate mucosal immune responses. Mucosal immunization has proven to be an effective approach against the colonization of pathogens and their further spread to the systemic circulation (7,20,23). Therefore, it is necessary to develop efficient and safe antigen vectors that will be able to trigger mucosal and systemic immune responses. One promising approach relies on the use of live bacterial vehicles (21). The potentiality of recombinant lactic acid bacteria to deliver heterologous antigens to the mucosal immune system has been investigated during the last decade (10,11,19,28,30,31,37). This approach offers a number of advantages (such as noninvasiveness and the possibility of eliciting both mucosal and systemic immune responses) over the traditional parenteral vaccination. In addition, lactobacilli have been used in a large variety of industrial food fermentation and preservation processes, are known for their beneficial effects on the health of humans and animals, ...

show abstract

Intragastric administration of Lactobacillus casei expressing transmissible gastroentritis coronavirus spike glycoprotein induced specific antibody production

Cited by 64 publications

References 32 publications

The Effects of Heat-Killed Wild-Type <i>Lactobacillus casei </i>Shirota on Allergic Immune Responses in an Allergy Mouse Model

The Effects of Heat-Killed Wild-Type <i>Lactobacillus casei </i>Shirota on Allergic Immune Responses in an Allergy Mouse Model

Immunogenicity of Lactobacillus-expressing VP2 and VP3 of the infectious pancreatic necrosis virus (IPNV) in rainbow trout

Induction of Immune Responses in Mice after Intragastric Administration of Lactobacillus casei Producing Porcine Parvovirus VP2 Protein

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