Intrageneric asymmetric hybrids between Nicotiana plumbaginifolia and Nicotiana sylvestris obtained by ‘gamma-fusion’

Famelaer, I.; Gleba, Yu. Yu.; Sidorov, V. A.; Kaleda, V. A.; Parokonny, A. S.; Boryshuk, N.V.; Cherep, N. N.; Negrutiu, Ioan; Jacobs, M.

doi:10.1016/0168-9452(89)90124-6

Cited by 64 publications

(20 citation statements)

References 19 publications

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“…The commonly used method for selection of asymmetric somatic hybrids involves irradiation (c-, g-ray or UV light) of the donor (Imamura et al 1987;Famelaer et al 1989;Vlahova et al 1997;Forsberg et al 1998) and chemical treatment (such as the metabolic inhibitor IOA) Fig. 7a, b F 1 hybrid mature plant from the small seed.…”

Section: Discussionmentioning

confidence: 99%

Asymmetric somatic hybridization between wheat (Triticum aestivum L.) and Agropyron elongatum (Host) Nevishi

et al. 2003

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Suspension-derived protoplasts of Agropyron elongatum irradiated by ultra-violet light (UV) were fused with the suspension-derived protoplasts of Triticum astivum using PEG. Fertile intergeneric somatic hybrid plants were produced and various hybrid lines have been selected and propagated in successive generations. Their hybrid nature was confirmed by analysis of profiles of isozymes, RAPDs, and 5S rDNA spacer sequences, and via GISH analysis. By the procedure described, the phenotype and chromosome number of wheat could be maintained besides transfer of a few chromosomes and chromosomal fragments from the donor A. elongatum. The results above indicated that highly asymmetric fertile hybrid plants and hybrid progenies of wheat were produced via somatic hybridization.

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Section: Discussionmentioning

confidence: 99%

Asymmetric somatic hybridization between wheat (Triticum aestivum L.) and Agropyron elongatum (Host) Nevishi

et al. 2003

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“…Although it has been well documented that irradiation leads to a preferential loss of donor DNA , the genetic components of asymmetric hybrids were very variable. In some fusion combinations most donors DNA is retained (Famelaer et al 1989;Wolters et al 1991;McCabe et al 1993) while in others chromosomes were eliminated more thoroughly leading to highly asymmetric lines (Dudits et al 1987;Vlahova et al 1997;Xia et al 2003).…”

Section: Introductionmentioning

confidence: 98%

Production and characterization of asymmetric hybrids between upland cotton Coker 201 (Gossypium hirsutum) and wild cotton (G. klozschianum Anderss)

Yang

Zhang

Jin

et al. 2007

Plant Cell Tiss Organ Cult

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Asymmetric somatic hybrids were obtained between Gossypium hirsutum Coker 201 and wild cotton G. klozschianum Anderss. An investigation on the effect of ultraviolet (UV) irradiation on donor protoplasts was carried out, and the lethal dose was determined to be 38.7 J cm À2 . We firstly screened the putative hybrids by the color of the calli produced, followed by morphological, cytological, and molecular analysis of putative hybrid plants. Most regenerated plants derived from fused protoplasts displayed a recipient-like morphology, while some showed an intermediate phenotype between Coker 201 and G. klozschianum. Chromosome numbers in these somatic hybrids ranged from 54 to 74. The hybrids were verified by random amplified polymorphic DNA (RAPD) and simple sequence repeat (SSR). Absence or co-existence of parents' genome DNA fragments was identified through molecular analysis. The heredity of cytoplasm was investigated by cleaved amplified polymorphic sequence (CAPS) analysis using mitochondrial and chloroplast universal primer pairs. The results indicated that recombination and rearrangements might have occurred in some regions of mitochondria (mt) and chloroplast (cp) DNA. To our knowledge, this is the first report about asymmetric protoplast fusion in cotton, and the hybrids obtained would be useful for breeding programs.

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“…To date, asymmetric hybridization has met with mixed results . In general, irradiation reduced the amount of transferred donor DNA but the fraction may vary from a few traits (Dudits et al ., 1987), one or a few chromosomes (Gupta et al ., 1984 ;Bates et al ., 1987a;Melzer & O'Connell, 1992) to numerous chromosomes (Gleba et al ., 1988 ;Famelaer et al ., 1989;Sacristan et al ., 1989 ;Sjodin & Glimelius, 1989b ;Yamashita et al ., 1989 ;Wijbrandi et al ., 1990a, b, c, Wolters et al ., 1991Derks et al ., 1992) the latter result being obtained in the majority of cases . Furthermore, chromosome elimination is random and it is not possible to predict which of the chromosomes will be lost .…”

Section: Symmetric Vs Asymmetric Hybridsmentioning

confidence: 99%

The potential of somatic hybridization in crop breeding

Waara

Glimelius

1995

Euphytica

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In recent years, the rapid development of somatic cell genetics has made possible the transfer of alien genes over wide taxonomic distances by somatic hybridization . In this review, the potential of somatic hybridization in the breeding of crops within the Brassicaceae, Fabaceae, Poaceae and Solanaceae is discussed . It is evident from these studies that many hybrids, either symmetric or asymmetric, which are fertile have the potential to be used as a bridge between the alien species and the crop . Progeny analysis of some hybrid combinations also reveals intergenomic translocations which may lead to the introgression of the alien genes . Furthermore, fusion techniques enable the resynthesis of allopolyploid crops to increase their genetic variability and to restore ploidy level and heterozygosity after breeding at reduced ploidy level in polyploid crops.

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Intrageneric asymmetric hybrids between Nicotiana plumbaginifolia and Nicotiana sylvestris obtained by ‘gamma-fusion’

Cited by 64 publications

References 19 publications

Asymmetric somatic hybridization between wheat (Triticum aestivum L.) and Agropyron elongatum (Host) Nevishi

Asymmetric somatic hybridization between wheat (Triticum aestivum L.) and Agropyron elongatum (Host) Nevishi

Production and characterization of asymmetric hybrids between upland cotton Coker 201 (Gossypium hirsutum) and wild cotton (G. klozschianum Anderss)

The potential of somatic hybridization in crop breeding

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