1986
DOI: 10.1002/j.1460-2075.1986.tb04580.x
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Intragenic pausing and anti-sense transcription within the murine c-myc locus.

Abstract: We present a detailed analysis of strand‐specific transcription in different regions of the murine c‐myc locus. In normal and transformed cell lines, RNA polymerase II directed transcription occurs in the sense and anti‐sense direction. Three noncontiguous regions show a high level of transcription in the anti‐sense orientation: upstream of the first exon, within the first intron and in the 3′ part of the gene (intron 2 and exon 3). In a cell line carrying a c‐myc amplification (54c12), anti‐sense transcriptio… Show more

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Cited by 314 publications
(166 citation statements)
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“…This would suggest a block to elongation at the exon 1/intron 1 boundary which prevents complete c-myc gene transcription in nonactivated lymphocytes. Similar results were obtained with HL-60 and 54cl2 {3T3) cells [24,30,33,34] which, however, possess amplified c-myc genes. Nuclear run-on analyses also showed a significant transcription of RNA complementary to c-myc mRNA, again only in nonactivated cells (Fig.…”
Section: Discussionsupporting
confidence: 83%
See 1 more Smart Citation
“…This would suggest a block to elongation at the exon 1/intron 1 boundary which prevents complete c-myc gene transcription in nonactivated lymphocytes. Similar results were obtained with HL-60 and 54cl2 {3T3) cells [24,30,33,34] which, however, possess amplified c-myc genes. Nuclear run-on analyses also showed a significant transcription of RNA complementary to c-myc mRNA, again only in nonactivated cells (Fig.…”
Section: Discussionsupporting
confidence: 83%
“…5A, lane 1, probe c). In centrast to the reports by Bentley and Groudine [24] and Nepveu and Marcu [34], we could detect anti-sense RNA transcription only with probe c which is specific for the exon 1/intron 1 boundary of the c-myc gene.…”
Section: Discussionsupporting
confidence: 70%
“…It has been reported, for instance for the c-myc gene [30], that some transcription of the coding strand of a gene could sometimes be observed. So, we looked by S1 nuclease mapping (Fig.…”
Section: S L Nuclease Mapping and Primer Extensionmentioning
confidence: 99%
“…A blockage or pause during elongation prevents formation of the full-length message; relief of that blockage is required for high-level expression of the gene. This type of transcriptional control has been found in a variety of eukaryotic genes and organisms, including human immunodeficiency virus type 1(1), human and murine c-myc (2)(3)(4) and ADA (5,6), and Drosophila hsp7O (7). (See ref.…”
mentioning
confidence: 99%