Intraluminal measurement of papillary duct urine pH, in vivo: a pilot study in the swine kidney

Handa, Rajash K.; Lingeman, James E.; Bledsoe, Sharon B.; Evan, Andrew P.; Connors, Bret A.; Johnson, Cynthia D.

doi:10.1007/s00240-015-0834-9

Cited by 7 publications

(8 citation statements)

References 24 publications

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“…In vivo fiber-optic pH sensing has been used previously in dogs [ 46 ], mice [ 47 ], swine [ 48 ], and humans [ 49 , 50 ]. This is the first study to our knowledge to measure extracellular pH in the respiratory tracts of pH1N1-infected animals.…”

Section: Discussionmentioning

confidence: 99%

Interplay between H1N1 influenza a virus infection, extracellular and intracellular respiratory tract pH, and host responses in a mouse model

et al. 2021

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During influenza A virus (IAV) entry, the hemagglutinin (HA) protein is triggered by endosomal low pH to undergo irreversible structural changes that mediate membrane fusion. HA proteins from different isolates vary in the pH at which they become activated in endosomes or become irreversible inactivated if exposed to extracellular acid. Little is known about extracellular pH in the upper respiratory tracts of mammals, how pH may shift during IAV infection, and its impact on replication of viruses that vary in HA activation pH. Here, we inoculated DBA/2J mice intranasally with A/TN/1-560/2009 (H1N1) (activation pH 5.5) or a mutant containing the destabilizing mutation HA1-Y17H (pH 6.0). We measured the kinetics of extracellular pH during infection using an optical pH-sensitive microsensor probe placed in the naris, nasal sinus, soft palate, and trachea. We also measured intracellular pH of single-cell suspensions of live, primary lung epithelial cells with various wavelength pH-sensitive dyes localized to cell membranes, cytosol, endosomes, secretory vesicles, microtubules, and lysosomes. Infection with either virus decreased extracellular pH and increased intracellular pH. Peak host immune responses were observed at 2 days post infection (DPI) and peak pH changes at 5 DPI. Extracellular and intracellular pH returned to baseline by 7 DPI in mice infected with HA1-Y17H and was restored later in wildtype-infected. Overall, IAV infection altered respiratory tract pH, which in turn modulated replication efficiency. This suggests a virus-host pH feedback loop that may select for IAV strains containing HA proteins of optimal pH stability, which may be approximately pH 5.5 in mice but may differ in other species.

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Section: Discussionmentioning

confidence: 99%

Interplay between H1N1 influenza a virus infection, extracellular and intracellular respiratory tract pH, and host responses in a mouse model

et al. 2021

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“…This optical pH sensing system has demonstrated success in accurately measuring pH values in blood volumes as low as 15 lL, and has been used previously to measure BD pH in a porcine model. 3,5,6 Before use, all pH sensors were calibrated and then sterilized with ethylene oxide. 3 In all cases, pH measurements were first taken from catheterized bladder urine collected at the beginning of the procedure without the use of irrigant.…”

Section: Methodsmentioning

confidence: 99%

“…3,5,6 Before use, all pH sensors were calibrated and then sterilized with ethylene oxide. 3 In all cases, pH measurements were first taken from catheterized bladder urine collected at the beginning of the procedure without the use of irrigant. Additional measurements were taken from the physiologic saline irrigant itself, used to facilitate visualization through the rigid and flexible nephroscope used during the procedure.…”

Section: Methodsmentioning

confidence: 99%

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In Vivo Renal Tubule pH in Stone-Forming Human Kidneys

Borofsky

Handa

Evan

et al. 2020

Journal of Endourology

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Introduction: There is evidence that patients with a history of ileostomies, who produce acidic urine and form uric acid or calcium oxalate stones, may plug some collecting ducts with calcium phosphate (CaP) and urate crystals. This is a paradoxical finding as such minerals should not form at an acid pH. One possible explanation is the presence of acidification defects due to focal damage to inner medullary collecting duct and Bellini duct (BD) cells. We sought to further investigate this hypothesis through direct measurement of ductal pH in dilated BDs in patients with ileostomies undergoing percutaneous nephrolithotomy (PCNL) for stone removal. Methods: After obtaining institutional review board approval, we used a fiber-optic pH microsensor with a 140lm-diameter tip to measure intraluminal pH from the bladder, saline irrigant, and dilated BDs of patients undergoing PCNL. Results: Measurements were taken from three patients meeting inclusion criteria. Measured pH of bladder urine ranged from 4.97 to 5.58 and pH of saline irrigant used during surgery ranged from 5.17 to 5.75. BD measurements were achieved in 11 different BDs. Mean intraductal BD pH was more than 1 unit higher than bulk urine (6.43 -0.22 vs 5.31 -0.22, p < 0.01). Conclusions: This is the first evidence for focal acidification defects within injured/dilated BDs of human kidneys producing highly acidic bulk phase urine. These results may help explain the paradoxical finding of CaP and urate plugs in dilated ducts of patients with stone-forming diseases characterized by highly acidic urine.

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“…The first fiber-optic pH sensor, described more than 40 years ago by Peterson et al, was developed for pH measurement in blood [ 5 ]. Recently, applications of fiber-optic sensors were described for pH measurement in brain tissue [ 6 ], lungs [ 7 , 8 , 9 ], bladder and kidneys [ 10 , 11 ], oocytes [ 12 ], blood and subcutaneous tissue [ 13 , 14 ], breast tissue [ 15 ] and in lung cancer cells [ 16 ].…”

Section: Introductionmentioning

confidence: 99%

Ex-Vivo Measurement of the pH in Aqueous Humor Samples by a Tapered Fiber-Optic Sensor

Podrazký

Mrázek

Proboštová

et al. 2021

Sensors

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A practical demonstration of pH measurement in real biological samples with an in-house developed fiber-optic pH sensor system is presented. The sensor uses 8-hydroxypyrene-1,3,6-trisulfonate (HPTS) fluorescent dye as the opto-chemical transducer. The dye is immobilized in a hybrid sol-gel matrix at the tip of a tapered optical fiber. We used 405 nm and 450 nm laser diodes for the dye excitation and a photomultiplier tube as a detector. The sensor was used for the measurement of pH in human aqueous humor samples during cataract surgery. Two groups of patients were tested, one underwent conventional phacoemulsification removal of the lens while the other was subjected to femtosecond laser assisted cataract surgery (FLACS). The precision of the measurement was ±0.04 pH units. The average pH of the aqueous humor of patients subjected to FLACS and those subjected to phacoemulsification were 7.24 ± 0.17 and 7.31 ± 0.20 respectively.

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Intraluminal measurement of papillary duct urine pH, in vivo: a pilot study in the swine kidney

Cited by 7 publications

References 24 publications

Interplay between H1N1 influenza a virus infection, extracellular and intracellular respiratory tract pH, and host responses in a mouse model

Interplay between H1N1 influenza a virus infection, extracellular and intracellular respiratory tract pH, and host responses in a mouse model

In Vivo Renal Tubule pH in Stone-Forming Human Kidneys

Ex-Vivo Measurement of the pH in Aqueous Humor Samples by a Tapered Fiber-Optic Sensor

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