Intralymphatic Administration of &lt;b&gt;&lt;i&gt;Metagonimus yokogawai&lt;/i&gt;&lt;/b&gt;-Extracted Protein Attenuates Experimental Murine Allergic Rhinitis Model

Yang, Hyung Chae; Won, Eun Jeong; Kim, Moon‐Ju; Sung, Chung Ki; Rhee, Joon Haeng; Nam, Kwang Il

doi:10.1159/000511532

Cited by 5 publications

(8 citation statements)

References 22 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…In addition, MPTP induced an increment in IL-17 and IFN-γ, and decrease in the TGF-β of PD mice. Since IL-17, IFN-γ and TGF-β are well known as the markers of Th17, Th1 and Treg [21], respectively, it was suggested that the immunoreactions of peripheral organs participated in the neuroinflammatory and neurodegenerative progress of PD. Of note, Th1 and Th17, the CD4 effector T cells, have been considered as the potential contributors to neuroinflammation in PD [10], and microgliosis can exacerbate the deterioration of DA neuron via a proinflammatory mediator, such as IFN-γ released from Th1 [22].…”

Section: Discussionmentioning

confidence: 99%

Chicoric Acid Prevents Neuroinflammation and Neurodegeneration in a Mouse Parkinson’s Disease Model: Immune Response and Transcriptome Profile of the Spleen and Colon

Wang

Feng

et al. 2022

IJMS

View full text Add to dashboard Cite

Chicoric acid (CA), a polyphenolic acid compound extracted from chicory and echinacea, possesses antiviral, antioxidative and anti-inflammatory activities. Growing evidence supports the pivotal roles of brain–spleen and brain–gut axes in neurodegenerative diseases, including Parkinson’s disease (PD), and the immune response of the spleen and colon is always the active participant in the pathogenesis and development of PD. In this study, we observe that CA prevented dopaminergic neuronal lesions, motor deficits and glial activation in PD mice, along with the increment in striatal brain-derived neurotrophic factor (BDNF), dopamine (DA) and 5-hydroxyindoleacetic acid (5-HT). Furthermore, CA reversed the level of interleukin-17(IL-17), interferon-gamma (IFN-γ) and transforming growth factor-beta (TGF-β) of PD mice, implicating its regulatory effect on the immunological response of spleen and colon. Transcriptome analysis revealed that 22 genes in the spleen (21 upregulated and 1 downregulated) and 306 genes (190 upregulated and 116 downregulated) in the colon were significantly differentially expressed in CA-pretreated mice. These genes were functionally annotated with GSEA, GO and KEGG pathway enrichment, providing the potential target genes and molecular biological mechanisms for the modulation of CA on the spleen and gut in PD. Remarkably, CA restored some gene expressions to normal level. Our results highlighted that the neuroprotection of CA might be associated with the manipulation of CA on brain–spleen and brain–gut axes in PD.

show abstract

Section: Discussionmentioning

confidence: 99%

Chicoric Acid Prevents Neuroinflammation and Neurodegeneration in a Mouse Parkinson’s Disease Model: Immune Response and Transcriptome Profile of the Spleen and Colon

Wang

Feng

et al. 2022

IJMS

View full text Add to dashboard Cite

show abstract

“…In this model, 25 µg IP OVA was given to Balb/c strain mice on days 0, 7 and 14, followed by intranasal OVA was administered on days 15, and 21 (19) . Another study investigated the potential therapeutic effect of Metagonimus yokogawai extract on the OVA-induced allergic rhinitis model (20) . In this study, 25 µg OVA were given IP 4 times on days 0, 7 and 14 to Balb/c type mice (20) .…”

Section: Discussionmentioning

confidence: 99%

“…Another study investigated the potential therapeutic effect of Metagonimus yokogawai extract on the OVA-induced allergic rhinitis model (20) . In this study, 25 µg OVA were given IP 4 times on days 0, 7 and 14 to Balb/c type mice (20) . Then, AR was induced by giving intranasal OVA every day from the 25 th to the 29 th day (20) .…”

Section: Discussionmentioning

confidence: 99%

See 1 more Smart Citation

A New Experimental Allergic Rhinitis Model in Mice

Köse¹,

Kiş²,

Diniz³

et al. 2021

BUCH

View full text Add to dashboard Cite

Objective: Allergic rhinitis (AR) is an inflammatory disease of the nasal mucosa mediated by IgE after exposure to an allergen. The most well known related comorbidity of AR is asthma. This study was planned due to the need for an animal model for studies on AR-asthma coexistence. In this study, the frequency of AR accompanying in the asthma model created in mice,and the usability of the related model in AR studies will be investigated. Methods: In our study, 6-8 week-old, 18-20 g BALB/c mice were used. Chicken egg ovalbumin (OVA Grade V, Sigma) was administered through intraperitoneal (IP) route at doses of 10 μg on days 0 and 14. Mice were exposed to aerosolized 2.5% ovalbumin solution in sterile saline for 30 minutes 3 days a week for 8 weeks, starting 7 days after the last IP administration (21st day). After exposure to OVA, mice were observed for typical signs of AR including sneezing, runny nose, and nasal itching. The final diagnosis of AR was made by histopathological examination of the rhinotracheal tissues of mice. Results: In our study, all mice exposed to ovalbumin received histopathologic diagnosis of AR. Increased number of capillaries lymphocytes, polymorphonuclear leukocytes and eosinophilsper square millimetre of rhinotracheal tissues were calculated in the murine model of AR compared to the the control group. Conclusion: This study introduced a new AR model, not cited in the literature, and induced with the longest-term ovalbumin exposure in the literature. It was concluded that this model, known as the asthma model, can also be used to induce an AR model and can be used in studies investigating coexistence of allergic rhinitis and asthma.

show abstract

“…The M. yokogawai metacercariae were collected from the muscles of Plecoglossus altivelis caught in the Seomjin-gang river in Jeollanamdo, South Korea, which is known to be an endemic area. The process of preparing the metacercariae was carried out as described previously [15]. The sh esh was treated with an arti cial digestive solution that contained 0.6% pepsin and 0.8% HCl at 37°C for two hours to obtain the metacercariae.…”

Section: Yokogawai and Their Antigen Preparationmentioning

confidence: 99%

A protein extracted from Metagonimus yokogawai alleviates inflammation in patients with Ankylosing Spondylitis

Won

Lee

Kim

et al. 2023

Preprint

Self Cite

View full text Add to dashboard Cite

Background Helminth infections and their components has been recognized to have a positive impact on the immune system. This study aimed to investigate the potential of Metagonimus yokogawai-derived proteins (MYp) to provide protection against ankylosing spondylitis (AS) through modulation of immune responses. Methods The cytotoxicity of MYp at various doses was first assessed using MTS and flow cytometry. Peripheral blood mononuclear cells (PBMCs) were collected from AS patients, and the production of inflammatory cytokines was analyzed through flow cytometry. In the experiments with SKG mice, MYp or vehicle was administered and inflammation was evaluated through immunohistochemistry and enzyme-linked immunosorbent assay. Results The results showed that MYp did not decrease cell viability of PBMCs even after 48 hours. Additionally, the frequencies of IFN-γ and IL-17A producing cells were significantly reduced after MYp treatment in the PBMC cultures. In the SKG mouse model, MYp treatment could reduce serum levels of INF- γ, IL-17A, and TNF-α. Overall, MYp treatment significantly suppressed arthritis and enthesitis in the SKG mouse model. Conclusions The results suggest the first evidence that MYp can effectively alleviate clinical symptoms and restore cytokine balance in patients with AS.

show abstract

Intralymphatic Administration of <b><i>Metagonimus yokogawai</i></b>-Extracted Protein Attenuates Experimental Murine Allergic Rhinitis Model

Cited by 5 publications

References 22 publications

Chicoric Acid Prevents Neuroinflammation and Neurodegeneration in a Mouse Parkinson’s Disease Model: Immune Response and Transcriptome Profile of the Spleen and Colon

Chicoric Acid Prevents Neuroinflammation and Neurodegeneration in a Mouse Parkinson’s Disease Model: Immune Response and Transcriptome Profile of the Spleen and Colon

A New Experimental Allergic Rhinitis Model in Mice

A protein extracted from Metagonimus yokogawai alleviates inflammation in patients with Ankylosing Spondylitis

Contact Info

Product

Resources

About