Intramolecular Photoelectrochemical System Using Tyrosine-Modified Antibody-Targeted Peptide as Electron Donor for Detection of Biomarkers

Abstract

An intramolecular photoelectrochemical (PEC) system is designed from the novel electron donor YYYHWRGWV (Y3-H) peptide ligand for the first time. The bifunctional nonapeptide cannot only rely on the HWRGWV sequence as a site-oriented immobilizer to recognize the crystallizable fragment (Fc) domains of the antibody but also acts as electron donors for PEC generation via three tyrosine (Y) of the N-terminal. The Bi 2 WO 6 /AgInS 2 heterojunction with a significant visible-light absorption is utilized as a photoelectric generator, and the motivation is ascribed to a proven proposition, namely, that short-wavelength illuminant radiates proteins, causing a decline in bioactivity of immune protein. An innovative biosensor is fabricated using the above strategies for the detection of CYFRA21-1, a biomarker of squamous cell lung carcinoma. This sort of PEC-based sensing platform shows convincing experimental data and could be an effective candidate for clinical application in the future due to their extremely skillful conception.

ZnAgInS Quantum Dot-Decorated BiOI Heterostructure for Cathodic Photoelectrochemical Bioanalysis of Glucose Oxidase

“…Semiconductor quantum dots (QDs) have demonstrated to be excellent light-harvesting materials due to their large surface to volume ratios and short charge transfer distances. , The specific size and property of QDs will promote effective contact area and charge separation efficiency. , Recently, cadmium-free AgInS 2 QDs, as typical emerging materials among ternary I–III–VI semiconductor QDs, have attracted intensive attention due to their low toxicity, superior band-gap tunability, large absorption coefficient, and relatively favorable environmental stability. − Especially, modification of AgInS 2 with ZnS to form quaternary ZnAgInS (ZAIS) QDs can effectively reduce the surface defects/traps and further improve their photocatalytic property and photo/chemical stability. , By virtue of these superiority, ZAIS QDs exhibit great promise for constructing a Z-scheme heterostructure with BiOI and being applied in the field of cathodic PEC bioassay . As far as we know, the BiOI-ZAIS heterostructure has not been studied.…”

Tailoring of the photocatalytic activity of CeO2 nanoparticles by the presence of plasmonic Ag nanoparticles

“…As an alternative, mediators can also be used for the communication of enzymes with semiconductor nanostructures -first examples have been reported here 18 . In addition to enzymes, also biospecific binding reactions have been coupled to photoactive electrodes, enlarging the applicability of such systems even further [19][20][21][22] .…”

“…18 In addition to enzymes, also biospecific binding reactions have been coupled to photoactive electrodes, enlarging the applicability of such systems even further. [19][20][21][22] There is a variety of semiconductor nanostructures which have been used for the construction of such photoelectrochemical schemes. Examples are quantum dots, 6,22,23 nanowires, 8,24,25 nanoclusters, [26][27][28] and others.…”

“…18 In addition to enzymes, also biospecific binding reactions have been coupled to photoactive electrodes, enlarging the applicability of such systems even further. 19–22…”

Tailoring the Photoelectrochemical Activity of Hexametaphosphate-Capped CdS Quantum Dots by Ca2+-Triggered Surface Charge Regulation: A New Signaling Strategy for Sensitive Immunoassay

“…Photoelectrochemical (PEC) analysis has attracted much interest in recent years due to its advantages including low background noise, high sensitivity, simple instrument, and easy operation. − A PEC immunoassay is a valuable analytical technique that combines the high sensitivity of PEC analysis with the high specificity of immunological reactions. − In a PEC immunoassay, immunobinding events directly or indirectly affect the electrolyte environment, the photoactive materials, and the interfacial electron/mass transfer, resulting in the increase or decrease of PEC signals. − By using an appropriate signaling strategy, the relationship between the target concentration and the PEC signal can be established. − The signaling strategy greatly determines the analytical performance and widespread applications of the PEC immunoassay, , so the development of an efficient signaling strategy is highly important.…”