Intranasal immunization with formalin-inactivated virus vaccine induces a broad spectrum of heterosubtypic immunity against influenza A virus infection in mice

Takada, Ayato; Matsushita, Sachiko; Ninomiya, Ai; Kawaoka, Yoshihiro; Kida, Hiroshi

doi:10.1016/s0264-410x(03)00234-2

Cited by 157 publications

(90 citation statements)

References 35 publications

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“…From the inactivated influenza vaccines, whole inactivated virus (WIV) formulations seem to be the most promising (5)(6)(7)(8). In comparison to subunit and split influenza vaccines, WIV induces much stronger humoral and cellular immune responses via various immunization routes (8)(9)(10)(11).…”

Section: Introductionmentioning

confidence: 99%

“…In comparison to subunit and split influenza vaccines, WIV induces much stronger humoral and cellular immune responses via various immunization routes (8)(9)(10)(11). Furthermore, WIV can induce cross-protection, especially when administered via the mucosal route, which may be especially important in the development of a pandemic vaccine (2,5,12,13). The superior, Th1-biased immune responses can be explained by the presence of viral RNA inside the particulate viral structure of WIV, a TLR 7 ligand, which acts as an adjuvant (10).…”

Section: Introductionmentioning

confidence: 99%

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Physicochemical and Immunological Characterization of N,N,N-Trimethyl Chitosan-Coated Whole Inactivated Influenza Virus Vaccine for Intranasal Administration

et al. 2009

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Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Physicochemical and Immunological Characterization of N,N,N-Trimethyl Chitosan-Coated Whole Inactivated Influenza Virus Vaccine for Intranasal Administration

et al. 2009

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“…For formalin-inactivated virus vaccines, the purified viruses were treated with 0.1% formalin at 4°C for a week. Protein concentrations of each vaccine were measured and standardized based on optical density (OD) at 280 nm with Protein Assay Kit (Bio-Rad, UK), and hemagglutinin (HA) units as described previously [22].Each virus contained 100-200 HA units in 100 µg of purified viral proteins. Inactivation of the virus in each vaccine preparation was confirmed by the absence of detectable HA activity following inoculation of the treated materials into 10 embryonated eggs.…”

mentioning

confidence: 99%

mentioning

confidence: 99%

Comparison of Antibody Titers in Rabbits Following Immunization with Inactivated Influenza Virus via Subarachnoidal or Subcutaneous Route

Shin

Sakoda

Kim

et al. 2007

The Journal of Veterinary Medical Science

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ABSTRACT. Rabbits were immunized with inactivated influenza virus via the subarachnoidal (SA) or subcutaneous (SC) route, and the antibody titers in cerebrospinal fluid (CSF) and serum were assayed. There were no nervous signs or morphological lesions related to SA immunization. In the SC group, the antibody titer was elevated in serum, but not elevated in CSF. In the SA group, the antibody titer was significantly elevated in serum and even in CSF, and their antibody titers were much greater than in the SC group. The present results suggest that intrathecal immunization is more effective than SC immunization at inducing a protective immune response against the transneural spread of viruses. [13,14,16,21]. Also, Yao et al. [25] reported that encephalitis was observed within 3-5 days after the intranasal infection of new mice with A/Aichi/ 2/68 INFV (H3N2) strain. The CNS and peripheral nerves are protected from impact by cerebrospinal fluid (CSF) [18]. The CNS is an immunologically privileged site and serum antibody does not influx into CSF under normal conditions due to blood-brain barrier (BBB) [7,12]; therefore, those neurotropic infectious agents may not meet the antibody response after they invade the nervous tissue. Serum antibody is effective after the pathogens are elicited on inflammation, which increases BBB permeability.Direct inoculation of antigens into CSF caused intrathecal immunization. We have previously shown that intrathecal immunization with inactivated pseudorabies virus, a neurotropic virus, completely protected the animals against lethal virus challenge, whereas all non-immunized and several subcutaneously immunized mice died after developing neurological signs [20]. Previous reports have shown that immunization via the brain or CSF elicits systemic humoral immune responses in the cervical lymph nodes, spleen, serum and CSF [7,8,19]. Furthermore, antigens are more immunogenic when administered into CNS than into conventional extracerebral sites. When CSF and serum antibody responses to albumin, which was administered into CSF or muscle, were compared with respect to the antibody titer in a rat model with normal BBB function, the CSF/ serum titer ratio and the ratio of immunoglobulin G subclasses were both elevated more prominently following CSF administration than intramuscular inoculation [1,2,7]. In this study, we compared the magnitude of the antibody responses in serum and CSF to inactivated INFV following immunization via the subarachnoidal (SA) or subcutaneous (SC) route, and examined the pathological effects of SA inoculation of INFV antigens in the brain.INFV, A/Aichi/2/68 INFV (H3N2) strain, was propagated in allantoic fluid of 10 day-old embryonated chicken eggs at 35°C for 48 hr. Virus was concentrated and purified by high-speed centrifugation, passed through a 10-50% sucrose density gradient, and resuspended in phosphatebuffered saline (PBS). For formalin-inactivated virus vaccines, the purified viruses were treated with 0.1% formalin at 4°C for a week. Protein concentrations o...

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“…Plaque assay for monitoring virus titers of homogenates of individual organs was performed as described previously (Takada et al, 2003). Briefly, 10% suspensions of the lung homogenates were examined.…”

Section: Virus Titermentioning

confidence: 99%

A Novel Glucan-Sulforaphane Combination Stimulates Immune Response to Influenza in Mouse Model

Větvička¹,

Větvičková²

2016

American Journal of Immunology

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Influenza remains a serious health problem and causes approximately 500,000 deaths world-wide. With current available treatments offering neither dependable protection nor a rapid cure, many have focused their attention to alternative treatments. The aim of this study was to evaluate the possible effect of a novel maitake glucan-sulforaphane combination on immune response against influenza challenge in mice. We evaluated the effects of a glucan-sulforaphane combination on basic immune reactions, virus titre and overall survival after influenza infection. Results: We found 2 weeks supplementation with this glucan-sulforaphane combination significantly improved immunosuppression caused by the viral infection. Based on these results, we conclude that the significant immunostimulation caused by this combination helps to overcome virusdependent suppression of defense reactions and that addition of sulforaphane to glucan can improve already established biological effects of glucan.

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Intranasal immunization with formalin-inactivated virus vaccine induces a broad spectrum of heterosubtypic immunity against influenza A virus infection in mice

Cited by 157 publications

References 35 publications

Physicochemical and Immunological Characterization of N,N,N-Trimethyl Chitosan-Coated Whole Inactivated Influenza Virus Vaccine for Intranasal Administration

Physicochemical and Immunological Characterization of N,N,N-Trimethyl Chitosan-Coated Whole Inactivated Influenza Virus Vaccine for Intranasal Administration

Comparison of Antibody Titers in Rabbits Following Immunization with Inactivated Influenza Virus via Subarachnoidal or Subcutaneous Route

A Novel Glucan-Sulforaphane Combination Stimulates Immune Response to Influenza in Mouse Model

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