Intranasal immunization with inactivated chlamydial elementary bodies formulated in VCG-chitosan nanoparticles induces robust immunity against intranasal Chlamydia psittaci challenge

Zuo, Zonghui; Zou, Yongjuan; Li, Qiang; Guo, Yue; Zhang, Tianyuan; Wu, Jie; He, Cheng; Eko, Francis O.

doi:10.1038/s41598-021-89940-8

Cited by 16 publications

(9 citation statements)

References 75 publications

(90 reference statements)

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“…Vibrio cholerae ghost (VCG) was manufactured, and the endotoxin was removed (Binzhou Animal Science and Veterinary Medicine Academy, Shandong Province, China) following previous reports (6). Chitosan gels were provided by Professor Wu Jie, Institute of Process Engineering, Chinese Academy of Sciences, Beijing, China.…”

Section: Adjuvants and Vaccine Formulationmentioning

confidence: 99%

“…As for vaccine formulation, the hydrogels and W/O droplet uniforms were prepared using Shirasu porous glass membrane emulsification. Afterward, 100 µg of recombinant antigens (50 µg of PmpGs + 50 µg of MOMP, or 100 µg of MOMP, or 100 µg of PmpGs), or inactivated EBs, 50 µg of VCG adjuvant and comparable volume of chitosan gels were mixed and blended completely as previously described (6). Finally, vaccines were stored at 4 • C until use.…”

Section: Adjuvants and Vaccine Formulationmentioning

confidence: 99%

“…C. psittaci-specific antibody levels were determined with customized inactivated EBs ELIAS kit as previously described (8). Regarding IgA antibody test, six throat swabs were collected from each group before the challenge test, and then samples were vortexed for 5 min with sterilized PBS, and C. psittaci-specific IgA antibody levels were analyzed using ELIAS assay as previously described (6).…”

Section: Humoral Responsementioning

confidence: 99%

“…Subsequently, the recombinant MOMP vaccines are not recognized due to partial protection for poultry and livestock. Recently, inactivated whole elemental body (EB) adjuvanted with VCG and chitosan induced better protection against C. psittaci infection in SPF chickens (6). Compared with intramuscular injection, intranasal immunity was identified to induce better protection post challenge with the virulent strain.…”

Section: Introductionmentioning

confidence: 99%

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A Novel Intranasal Vaccine With PmpGs + MOMP Induces Robust Protections Both in Respiratory Tract and Genital System Post Chlamydia psittaci Infection

Chen

Zhang

et al. 2022

Front. Vet. Sci.

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Chlamydia psittaci (C. psittaci) is a crucial zoonotic pathogen that causes severe respiratory and reproductive system disease in humans and animals. In our pioneer study, polymorphic membrane protein G (PmpG) mediated attachment to host cells as the adhesions and induced immunity against C. psittaci infection. We hypothesize that multiple PmpG antigens adjuvanted with Vibrio cholerae ghost (VCG) and chitosan gel might trigger full protection via the intranasal route (i.n). In the present study, 40 SPF chickens were randomly divided into four groups, including the PmpGs + MOMP group (i.n), major outer membrane protein (MOMP) group (i.n), PmpGs (Pmp17G + Pmp20G + Pmp21G) group (i.n), and control groups (VCG + chitosan gel) (i.n). Post twice immunizations, the PmpGs + MOMP group yielded highly level-specific IgG, IgA antibodies, and lymphocyte proliferation. As for cytokines, IFN-γ expression was upregulated significantly, while IL-10 concentration was downregulated in the PmpGs + MOMP group compared with other groups. Post challenge, exudate inflammations in air sacs, bacterial loads in lungs, and bacterial shedding in throat swabs were reduced significantly in the PmpGs + MOMP group. In the second experiment, 100 breeder ducks were divided into the PmpGs + MOMP group (i.n), the commercial MOMP group (via intramuscular injection, i.m), the inactivated EBs group (i.n), and the control group (i.n), 25 ducks per group. Post challenge, the reduced egg production recovered soon in the inactivated EBs group and the PmpGs + MOMP group. Moreover, the aforementioned two groups induced higher robust IgG antibodies, lymphocyte proliferation, and IFN-γ secretions than the commercial MOMP vaccine did. Postmortem, lower bacterial loads of spleens were determined in the PmpGs + MOMP group and the inactivated EBs group. However, bacterial clearance of follicular membranes and shedding from the vaginal tract were not significant differences among the three tested groups. Furthermore, the PmpGs + MOMP group induced lower inflammations in the follicles and oviducts. Based on the above evidence, the combination of PmpGs and MOMP adjuvanted with chitosan gel and VCG via intranasal route could induce full protection both in the respiratory system and genital tract post C. psittaci infection. More importantly, the combination antigens are superior to the inactivated EBs antigen due to no contamination to the environment and less genital inflammation. The combination of PmpGs + MOMP adjuvanted with VCG and chitosan gel might be a promising novel vaccine by blocking C. psittaci infection from animals to human beings.

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Section: Adjuvants and Vaccine Formulationmentioning

confidence: 99%

Section: Adjuvants and Vaccine Formulationmentioning

confidence: 99%

Section: Humoral Responsementioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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A Novel Intranasal Vaccine With PmpGs + MOMP Induces Robust Protections Both in Respiratory Tract and Genital System Post Chlamydia psittaci Infection

Chen

Zhang

et al. 2022

Front. Vet. Sci.

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“…38 Further, TMC coated inactivated polio vaccine prepared as pH-sensitive microneedle gave a promising antigen dose with significant antibody response, showing its suitability for dermal vaccination. 39 Studies on many new-gen vaccine administration routes (including nasal and intradermal vaccines) in combination with different forms of CS [40][41][42][43][44] have effec-tively highlighted its ability to act as a promising stabilizer as well as an adjuvant.…”

Section: Trimethyl Chitosanmentioning

confidence: 99%

Biopolymers and Osmolytes — A Focus towards the Prospects of Stability and Adjuvanticity of Vaccines

2022

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‘New-Gen Vaccines’ are grabbing the attention of scientists as they are much suitable for an immune-compromised group of individuals as well as infants. The major drawbacks of these vaccines are lower immunogenicity and instability. The need for a convenient and safe adjuvant is still under exploration. On the other hand, thermal instability leads to the inactivation of the vaccine and becomes detrimental in many cases. Thus, there is a need to incorporate new kinds of excipients into vaccine formulation to enhance the potency/immunogenicity of vaccine antigens and also act as stabilizers. A limited or single excipient in providing the required dual-activity is vital to break the stereotypical usage of the well-entrenched adverse ingredients. In the proposed review, the efficiency of naturally occurring biocompatible carbohydrate polymers and osmolytes and their ‘dual-role’ is briefed. In addition, the information on the possible mechanisms of action of carbohydrate polymers in vaccines as adjuvants and stabilizers are also discussed.

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Chitosan-Based Nanofertilizer: Types, Formulations, and Plant Promotion Mechanism

Nirmala,

Nayak,

Narasimhan

et al. 2023

Nanotechnology in the Life Sciences

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Intranasal immunization with inactivated chlamydial elementary bodies formulated in VCG-chitosan nanoparticles induces robust immunity against intranasal Chlamydia psittaci challenge

Cited by 16 publications

References 75 publications

A Novel Intranasal Vaccine With PmpGs + MOMP Induces Robust Protections Both in Respiratory Tract and Genital System Post Chlamydia psittaci Infection

A Novel Intranasal Vaccine With PmpGs + MOMP Induces Robust Protections Both in Respiratory Tract and Genital System Post Chlamydia psittaci Infection

Biopolymers and Osmolytes — A Focus towards the Prospects of Stability and Adjuvanticity of Vaccines

Chitosan-Based Nanofertilizer: Types, Formulations, and Plant Promotion Mechanism

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