Intranasal immunization with temperature-sensitive mutants protects granulocytopenic mice from lethal pulmonary challenge withPseudomonas aeruginosa

Sordelli, D O; García, Verónica; Cerquetti, M C; Fontán, Patricia A.; Hooke, Anne Morris

doi:10.1007/bf01570093

Cited by 12 publications

(5 citation statements)

References 14 publications

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“…Several useful models of acute and forced chronic P. aerugi-nosa infection have been described in normal, neutropenic, neonatal, or burned mice and in other animals (5,8,10,21,38,47,(49)(50)(51)53). Recently, an aerosol model of respiratory infections with Staphylococcus aureus and Burkholderia cepacia has been developed in the CFTR m1HGU transgenic mouse with a mild defect in the CFTR gene (11).…”

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confidence: 99%

Microbial Pathogenesis in Cystic Fibrosis: Pulmonary Clearance of MucoidPseudomonas aeruginosaand Inflammation in a Mouse Model of Repeated Respiratory Challenge

Hanes

Chrisp

et al. 1998

Infect Immun

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Chronic endobronchiolitis compounded by recurring Pseudomonas aeruginosa infections is the major cause of morbidity and mortality in patients with cystic fibrosis (CF). In this study, a mouse model of repeated respiratory exposure to P. aeruginosa was established to facilitate investigations of factors contributing toP. aeruginosa persistence and associated inflammatory processes in the lung. While a single exposure to P. aeruginosa aerosols resulted in only mild histopathological changes, repeated exposure caused significant lung pathology in C57BL/6J mice. The peak of histopathological changes and inflammation in C57BL/6J mice was characterized by subacute lymphohistiocytic bronchopneumonia and persistent elevation of tumor necrosis factor alpha and macrophage inflammatory protein 2 in the lung but not in the serum. When isogenic nonmucoid (mucA +) and mucoid (mucA22) P. aeruginosa strains were compared, the mucoid cells were cleared several-fold less efficiently than the parental nonmucoid strain during the initial stages of the aerosol exposure regimen. However, the microscopic pathology findings and proinflammatory cytokine levels were similar in mice exposed to nonmucoid and mucoid P. aeruginosa throughout the infection. We also tested lung histopathology and proinflammatory cytokines in interleukin 10 (IL-10)-deficient transgenic (IL-10T) mice. Significant mortality was seen in IL-10T mice on initial challenge withP. aeruginosa, although no histopathological differences could be observed in the lungs of C57BL/6J and surviving IL-10T mice after a single exposure. However, increased pathology was detected in IL-10T mice relative to C57BL/6J after repeated challenge with P. aeruginosa. This observation supports the proposals that anti-inflammatory cytokines may play a role in suppressing P. aeruginosa-induced tissue damage during chronic infection.

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confidence: 99%

Microbial Pathogenesis in Cystic Fibrosis: Pulmonary Clearance of MucoidPseudomonas aeruginosaand Inflammation in a Mouse Model of Repeated Respiratory Challenge

Hanes

Chrisp

et al. 1998

Infect Immun

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“…The presence of these antibodies did not modify the pulmonary clearance of S. aureus, but the concomitant presence of PMNL and antibodies in the airways resulted in improved clearance of the bacteria. In previous reports we have shown that local immunization of the respiratory tract with temperature-sensitive mutants enhances lung defenses against P. aeruginosa [17,[19][20][21]. This enhancement was associated with the presence of antibodies and PMNL in the respiratory tract.…”

Section: Discussionmentioning

confidence: 69%

“…In our laboratory we have shown that granulocytopenic mice were also protected by i.n. immunization, which demonstrated that the appearance of antibodies in the lung can provide protection from lethal challenge with P. aeruginosa [21] since cyclophosphamide treatment does not affect the alveolar macrophage population. The effect of specific antibodies and PMNL in the airways on the enhancement of lung clearance of S. aureus is, however, less clear.…”

Section: Discussionmentioning

confidence: 99%

Interaction between granulocytes and antibodies in the enhancement of lung defenses against Staphylococcus aureus after intranasal immunization of mice with live-attenuated bacteria

García¹

1994

FEMS Immunology and Medical Microbiology

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Immunization with live-attenuated Staphylococcus aureus induced measurable levels of specific IgG and IgA in the lungs, but the pulmonary clearance of S. aureus in immunized mice did not differ from that of control mice. Aerosol exposure of mice to Pseudomonas aeruginosa induced a significant recruitment of polymorphonuclear leukocytes (PMNL) to the lungs in both immunized and control mice, whereas S. aureus challenge did not. However, challenge with a mixture of P. aeruginosa-S. aureus or exposure to an aerosol of Escherichia coli lipopolysaccharide (LPS) before S. aureus challenge induced PMNL migration and a significant enhancement of pulmonary clearance of S. aureus in immunized mice. The presence of both antibodies and PMNL was required for enhancement of S. aureus pulmonary clearance.

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“…Di¡erent temperature-sensitive mutants of bacteria have been utilized as immunogens either to evoke an antibody response [19] or to achieve protection of the immunized host [8,10,11,20]. Our results showed that ts mutants of S. enteritidis protect mice against challenge with wild-type Salmonella strains of di¡erent O serotypes.…”

Section: Discussionmentioning

confidence: 74%

Salmonella enteritidis temperature-sensitive mutants protect mice against challenge with virulent Salmonella strains of different serotypes

Gherardi

2000

FEMS Immunology and Medical Microbiology

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The protection conferred by temperature-sensitive mutants of Salmonella enteritidis against different wild-type Salmonella serotypes was investigated. Oral immunization with the single temperature-sensitive mutant E/1/3 or with a temperature-sensitive thymine-requiring double mutant (E/1/3T) conferred: (i) significant protection against the homologous wild-type Salmonella strains; (ii) significant cross-protection toward high challenge doses of S. typhimurium. Significant antibody levels against homologous lipopolysaccharide and against homologous and heterologous protein antigens were detected in sera from immunized mice. Moreover, a wide range of protein antigens from different Salmonella O serotypes were recognized by sera from immunized animals. Besides, primed lymphocytes from E/1/3 immunized mice recognized Salmonella antigens from different serotypes. Taken together, these results indicate that temperature-sensitive mutants of S. enteritidis are good candidates for the construction of live vaccines against Salmonella.

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Intranasal immunization with temperature-sensitive mutants protects granulocytopenic mice from lethal pulmonary challenge withPseudomonas aeruginosa

Cited by 12 publications

References 14 publications

Microbial Pathogenesis in Cystic Fibrosis: Pulmonary Clearance of MucoidPseudomonas aeruginosaand Inflammation in a Mouse Model of Repeated Respiratory Challenge

Microbial Pathogenesis in Cystic Fibrosis: Pulmonary Clearance of MucoidPseudomonas aeruginosaand Inflammation in a Mouse Model of Repeated Respiratory Challenge

Interaction between granulocytes and antibodies in the enhancement of lung defenses against Staphylococcus aureus after intranasal immunization of mice with live-attenuated bacteria

Salmonella enteritidis temperature-sensitive mutants protect mice against challenge with virulent Salmonella strains of different serotypes

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