Intranasal inoculation with Bordetella pertussis confers protection without inducing classical whooping cough in baboons

Naninck, Thibaut; Contreras, Vanessa; Coutte, Loïc; Langlois, Sébastien; Hébert-Ribon, Aurélie; Pelletier, Magali; Reveneau, Nathalie; Locht, Camille; Chapon, Catherine; Grand, Roger Le

doi:10.1016/j.crmicr.2021.100072

Cited by 4 publications

(3 citation statements)

References 22 publications

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“…That mucosal protection is applicable to human prevention has been supported by the lower frequency of pertussis among offspring of pregnancies that have received antepartum vaccination (Zumstein et al 2021 ). Recent studies in primates also support a potential approach for direct mucosal immunization (Naninck et al 2021 ). Given that the majority of manifest disease is related to the airways, consideration may also be made for co-infections (Sawal et al 2009 ).…”

Section: Future Prospectsmentioning

confidence: 94%

Non-primate animal models for pertussis: back to the drawing board?

Cimolai

2022

Appl Microbiol Biotechnol

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Despite considerable progress in the understanding of clinical pertussis, the contemporary emergence of antimicrobial resistance for Bordetella pertussis and an evolution of concerns with acellular component vaccination have both sparked a renewed interest. Although simian models of infection best correlate with the observed attributes of human infection, several animal models have been used for decades and have positively contributed in many ways to the related science. Nevertheless, there is yet the lack of a reliable small animal model system that mimics the combination of infection genesis, variable upper and lower respiratory infection, systemic effects, infection resolution, and vaccine responses. This narrative review examines the history and attributes of non-primate animal models for pertussis and places context with the current use and needs. Emerging from the latter is the necessity for further such study to better create the optimal model of infection and vaccination with use of current molecular tools and a broader range of animal systems. Key points • Currently used and past non-primate animal models of B. pertussis infection often have unique and focused applications. • A non-primate animal model that consistently mimics human pertussis for the majority of key infection characteristics is lacking. • There remains ample opportunity for an improved non-primate animal model of pertussis with the use of current molecular biology tools and with further exploration of species not previously considered. Graphical abstract

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Section: Future Prospectsmentioning

confidence: 94%

Non-primate animal models for pertussis: back to the drawing board?

Cimolai

2022

Appl Microbiol Biotechnol

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“…Their formulations could be supplemented with detoxified versions of these proteins if their antigens are critical in Bp immunity, such as pertussis toxin. It is likely that immunomodulation is particularly important in mucosal immunity to Bp, as the tightly controlled inflammatory responses in the nasopharynx may be further reduced to enable persistence and extend the window of transmissibility (19,(58)(59)(60)(61).…”

Section: Disrupting Immunomodulation To Inform Vaccine-developmentmentioning

confidence: 99%

Generating enhanced mucosal immunity against Bordetella pertussis: current challenges and new directions

2023

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Bordetella pertussis (Bp) is the highly transmissible etiologic agent of pertussis, a severe respiratory disease that causes particularly high morbidity and mortality in infants and young children. Commonly known as “whooping cough,” pertussis is one of the least controlled vaccine-preventable diseases worldwide with several countries experiencing recent periods of resurgence despite broad immunization coverage. While current acellular vaccines prevent severe disease in most cases, the immunity they confer wanes rapidly and does not prevent sub clinical infection or transmission of the bacterium to new and vulnerable hosts. The recent resurgence has prompted new efforts to generate robust immunity to Bp in the upper respiratory mucosa, from which colonization and transmission originate. Problematically, these initiatives have been partially hindered by research limitations in both human and animal models as well as potent immunomodulation by Bp. Here, we consider our incomplete understanding of the complex host-pathogen dynamics occurring in the upper airway to propose new directions and methods that may address critical gaps in research. We also consider recent evidence that supports the development of novel vaccines specifically designed to generate robust mucosal immune responses capable of limiting upper respiratory colonization to finally halt the ongoing circulation of Bordetella pertussis.

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“…This tool is particularly useful for the study of persistence and spatiotemporal distribution of bacteria in living animals, as well as for monitoring bacterial infections (Romero Pastrana et al 2018 ). Such imaging can be performed either using endogenous, genetically encoded luciferase (bioluminescence) (Doyle et al 2004 ) or green fluorescent protein (GFP) (Naninck et al 2021 ) or using exogenous fluorescent tracers that target and/or illuminate bacteria (Van Oosten et al 2015 ). Fluorescence has several advantages in comparison to bioluminescence as it does not require the administration of luciferin, yields brighter signal, and is more appropriate for combining with microscopy or flow cytometry (Calvo-Álvarez et al 2015 ; Choy et al 2003 ).…”

Section: Introductionmentioning

confidence: 99%

In vivo monitoring of Lactiplantibacillus plantarum in the nasal and vaginal mucosa using infrared fluorescence

Silva-Bea¹,

Francisco-Tomé²,

Cabrera-Alvargonzález

et al. 2022

Appl Microbiol Biotechnol

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Lactic acid bacteria (LAB) of the genus Lactiplantibacillus have been explored as potential mucosal vaccine vectors due to their ability to elicit an immune response against expressed foreign antigens and to their safety. However, tools for monitoring LAB distribution and persistence at the mucosal surfaces are needed. Here, we characterize Lactiplantibacillus plantarum bacteria expressing the infrared fluorescent protein IRFP713 for exploring their in vivo distribution in the mucosa and potential use as a mucosal vaccine vector. This bacterial species is commonly used as a vaginal probiotic and was recently found to have a niche in the human nose. Three different fluorescent L. plantarum strains were obtained using the nisin-inducible pNZRK-IRFP713 plasmid which contains the nisRK genes, showing stable and constitutive expression of IRFP713 in vitro. One of these strains was further monitored in BALB/c mice using near-infrared fluorescence, indicating successful colonization of the nasal and vaginal mucosae for up to 72 h. This study thus provides a tool for the in vivo spatiotemporal monitoring of lactiplantibacilli, allowing non-invasive bacterial detection in these mucosal sites. Key points • Stable and constitutive expression of the IRFP713 protein was obtained in different L. plantarum strains. • IRFP713+ L. plantarum 3.12.1 was monitored in vivo using near-infrared fluorescence. • Residence times observed after intranasal and vaginal inoculation were 24–72 h. Graphical abstract

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Intranasal inoculation with Bordetella pertussis confers protection without inducing classical whooping cough in baboons

Cited by 4 publications

References 22 publications

Non-primate animal models for pertussis: back to the drawing board?

Non-primate animal models for pertussis: back to the drawing board?

Generating enhanced mucosal immunity against Bordetella pertussis: current challenges and new directions

In vivo monitoring of Lactiplantibacillus plantarum in the nasal and vaginal mucosa using infrared fluorescence

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