Intraneural IFG-1 in Cryopreserved Nerve Isografts Increase Neural Regeneration and Functional Recovery in the Rat Sciatic Nerve

Porto, Sara Alicia González; Doménech, Nieves; Blanco, Francisco J.; Cortés, Alberto Centeno; Rivadulla, Casto; Jorge, Ángel Álvarez; Ibáñez, Jacinto Sánchez; Vázquez, Esther Rendal

doi:10.1093/neuros/nyy339

Cited by 5 publications

(3 citation statements)

References 47 publications

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“…The results of electrophysiological experiments were recorded in 51% of the articles (Figure 2E). NCV is typically assessed through the measurement of CMAP amplitude in the motor nerve 50 . As the cumulative response of muscle fibers within the stimulated muscle, CMAP amplitude is influenced by both the integrity of the nerve fibers and the rate of depolarization 51 .…”

Section: Discussionmentioning

confidence: 99%

“…NCV is typically assessed through the measurement of CMAP amplitude in the motor nerve. 50 As the cumulative response of muscle fibers within the stimulated muscle, CMAP amplitude is influenced by both the integrity of the nerve fibers and the rate of depolarization. 51 Based on the overall pooled estimate, ADSCs exhibited significantly improved CMAP amplitude, as well as reduced latency.…”

Section: Key Indicators For Determining Repair Of Peripheral Nerve De...mentioning

confidence: 99%

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Combination of stem cells and nerve guide conduit for the treatment of peripheral nerve injury: A meta‐analysis

Cao,

Yihao,

et al. 2023

Muscle and Nerve

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Introduction/AimsMany small‐sized, single‐center preclinical studies have investigated the benefits of introducing stem cells into the interior of nerve conduit. The aims of this meta‐analysis are to review and contrast the effects of various types of stem cells in in vivo models used to reconstruct peripheral nerve injuries (PNIs) and to assess the reliability and stability of the available evidence.MethodsA systematic search was conducted using Cochrane Library, Embase, PubMed, and Web of Science to identify studies conducted from January 1, 2000, to September 21, 2022, and investigate stem cell therapy in peripheral nerve reconstruction animal models. Studies that met the relevant criteria were deemed eligible for this meta‐analysis.ResultsFifty‐five preclinical studies with a total of 1234 animals were incorporated. Stem cells demonstrated a positive impact on peripheral nerve regeneration at different follow‐up times in the forest plots of five outcome indicators: compound muscle action potential (CMAP) amplitude, latency, muscle mass ratio, nerve conduction velocity, and sciatic functional index (SFI). In most comparisons, stem cell groups showed substantial differences compared with the control groups. The superior performance of adipose‐derived stem cells (ADSCs) in terms of SFI, CMAP amplitude, and latency (p < .001) was identified.DiscussionThe findings consistently demonstrated a favorable outcome in the reconstruction process when utilizing different groups of stem cells, as opposed to control groups where stem cells were not employed.

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Section: Discussionmentioning

confidence: 99%

Section: Key Indicators For Determining Repair Of Peripheral Nerve De...mentioning

confidence: 99%

Combination of stem cells and nerve guide conduit for the treatment of peripheral nerve injury: A meta‐analysis

Cao,

Yihao,

et al. 2023

Muscle and Nerve

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“…Regeneration and migration of Schwann cells are also critical for peripheral nerve regeneration . For this reason, the local administration of IGF-1 is an important part of the recovery process after peripheral nerve injury. , Therefore, it is crucial to determine how to facilitate the slow release of CNTF and IGF-1 in the local injury milieu with the aim of enhancing nerve regeneration. The introduction of an exogenous slow-release system to slow the release of CNTF and IGF-1 is the key to solving this problem.…”

Section: Introductionmentioning

confidence: 99%

Electrospun PCL Nerve Conduit Filled with GelMA Gel for CNTF and IGF-1 Delivery in Promoting Sciatic Nerve Regeneration in Rat

Xu,

Gao,

Wang

et al. 2023

ACS Biomater. Sci. Eng.

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Neural tissue engineering is an essential strategy to repair long-segment peripheral nerve defects. Modification of the nerve conduit is an effective way to improve the local microenvironment of the injury site and facilitate nerve regeneration. However, the concurrent release of multiple growth cues that regulate the activity of Schwann cells and neurons remains a challenge. The present study involved the fabrication of a composite hydrogel, specifically methacrylate-anhydride gelatin-ciliary neurotrophic factor/insulin-like growth factor-1 (GelMA-CNTF/IGF-1), with the aim of providing a sustained release of CNTF and IGF-1. The GelMA-CNTF/IGF-1 hydrogels exhibited a swelling rate of 10.2% following a 24 h incubation in vitro. In vitro, GelMA hydrogels demonstrated a high degree of efficiency in the sustained release of CNTF and IGF-1 proteins, with a release rate of 85.9% for CNTF and 90.9% for IGF-1 shown at day 28. In addition, the GelMA-CNTF/IGF-1 composite hydrogel promoted the proliferation of Schwann cells and the production of nerve growth factor (NGF), connective tissue growth factor (CTGF), fibronectin, and laminin and also considerably promoted the axonal growth of neurons. Furthermore, GelMA-CNTF/IGF-1 hydrogels were loaded into PCL electrospun nerve conduits to repair 15 mm sciatic nerve defects in rats. In vivo studies indicated that PCL-GelMA-CNTF/IGF-1 could efficiently accelerate the regeneration of the rat sciatic nerve, promote the formation of the myelin sheath of new axons, promote the electrophysiological function of regenerated nerves, and eventually improve the recovery of motor function in rats. Overall, the PCL-GelMA-CNTF/IGF-1 scaffold presents an attractive new approach for generating an optimal therapeutic alternative for peripheral nerve restoration.

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Live Imaging of Primary Neurons in Long-Term Cryopreserved Human Nerve Tissue

Fortea

Jain

Demedts³

et al. 2021

eNeuro

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Tissue cryopreservation provides a convenient solution for tackling one of the major problems in neuroscience research, namely, the scarce availability of human nerve tissues, especially if needed alive. While brain tissue can be used only postmortem, live nerve tissue can reasonably well be harvested from the periphery. A valuable source of primary neurons is the intestine, which compared with brain has the advantage to be safely accessible via endoscopy. The nerve tissue innervating the intestine (the enteric nervous system; ENS) can be sampled with regular endoscopic biopsy forceps and remains viable for multiple physiological and immunohistochemical tests, as previously demonstrated. Here, we present a method to preserve, over longer periods of time, human primary neurons contained in these biopsies. The use of a cryoprotective agent and the application of controlled cooling revealed to be crucial to properly store the nerve tissue and to enable functional measurements after thawing. These primary neurons were evaluated for functionality (live imaging) and morphology (histology) up to one year after cryopreservation. Calcium (Ca2+) imaging indicated that human primary neurons remained viable and responded to selective stimulations (serotonergic and nicotinic agonists) after cryopreservation. Additionally, immunohistochemistry performed with specific neuronal markers showed that nerve structure and neuronal morphology were retained, with no signs of cellular damage. In this study, we demonstrate that the human ENS is a realistic source of primary neurons, which can be successfully preserved over long times and as such can be exploited both for gastrointestinal-specific as well as for general neuroscience research.

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Intraneural IFG-1 in Cryopreserved Nerve Isografts Increase Neural Regeneration and Functional Recovery in the Rat Sciatic Nerve

Abstract: The results show that intraneural injection of IGF-1 in an 18 mm cryopreserved isograft improve axonal regeneration and functional recovery.

Cited by 5 publications

References 47 publications

Combination of stem cells and nerve guide conduit for the treatment of peripheral nerve injury: A meta‐analysis

Combination of stem cells and nerve guide conduit for the treatment of peripheral nerve injury: A meta‐analysis

Electrospun PCL Nerve Conduit Filled with GelMA Gel for CNTF and IGF-1 Delivery in Promoting Sciatic Nerve Regeneration in Rat

Live Imaging of Primary Neurons in Long-Term Cryopreserved Human Nerve Tissue

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