1987
DOI: 10.1677/joe.0.1120161
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Intranuclear distribution of androgen receptors in human prostate carcinoma

Abstract: Androgen receptors in nuclei from human prostate carcinomas were characterized on the basis of their solubilization by, or resistance to, micrococcal nuclease. By this means, androgen receptors were assigned to three nuclear categories: those associated with nuclease-resistant structures, those associated with chromatin and those apparently uncommitted by association with either of these. Prostate carcinoma nuclei contained high concentrations (57-82% of total nuclear content) of nuclease-resistant androgen re… Show more

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Cited by 5 publications
(6 citation statements)
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“…Infrequently, BPH tissue was obtained after retropubic prostatectomy, and on these occasions tissues classified as histologically normal (HN) were derived from peripheral areas of these samples. These tissues were chopped into approximate 3 mm3 pieces [23], which were checked histologically to confirm assessment on whole tissues.…”
Section: Materials and Methods Tissuementioning
confidence: 99%
“…Infrequently, BPH tissue was obtained after retropubic prostatectomy, and on these occasions tissues classified as histologically normal (HN) were derived from peripheral areas of these samples. These tissues were chopped into approximate 3 mm3 pieces [23], which were checked histologically to confirm assessment on whole tissues.…”
Section: Materials and Methods Tissuementioning
confidence: 99%
“…1). Cancer cells containing a high proportion of nuclease-resistant receptors can exist in a varying environment of receptor-negative cancer cells and/or normal cells (in which the distribution of extractable to resistant receptors is 1:1 [8,14]), which would affect the resistant receptor to total receptor ratio in dif ferent ways. Fig.…”
Section: Resultsmentioning
confidence: 99%
“…Smaller fragments damaged by TUR were discarded and only those from which charred material could be removed to leave satisfacto rily uninjured samples were used [15]. Homogenization of tissue, preparation of nuclei and exhaustive nucleolysis to yield total pop ulations of nuclease-extractable and nuclease-resistant androgen re ceptors have been described [13,14], Under the conditions em ployed, 'nuclease-resistant' described those receptors which had not been solubilized during a digestion procedure following which no more nuclear material could be released by the action of micrococ cal nuclease (used routinely) or any other nuclease alone. In this report, 'nuclease-extractable' describes those androgen receptors which have been solubilized during such a procedure.…”
Section: Methodsmentioning
confidence: 99%
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“…The long-term, differentiation-related hormonal changes, however, argue against the participation of plasma membrane-based mechanisms because molecules involved in these pathways reach a peak in a few minutes and their effects last only for a couple of hours [13]. Chromatin involvement, on the other hand, was confirmed by receptor localization studies [14,15], receptor interaction assays [16] and by detection of global rearrangements of the chromatin architecture [17]. Hormone-induced chromatin changes, in turn, can regulate the expression of large sets of genes [18].…”
Section: Introductionmentioning
confidence: 99%