Intranuclear Inclusions in Cells Infected with Newcastle Disease Virus

Granzow, Harald; Weiland, Frank; Mundt, Egbert; Köllner, Bernd; Werner, Ortrud

doi:10.1046/j.1439-0450.1999.00248.x

Cited by 6 publications

(7 citation statements)

References 22 publications

(28 reference statements)

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“…The presence of intracytoplasmatic and intranuclear inclusions, surrounded by a membrane and containing viral nucleocapsid are in accordance with other authors who used the resin embedding technique (Mannl et al, 1987;Granzow et al, 1999;Jacobson et al, 2001). …”

Section: Discussionsupporting

confidence: 90%

Avian Paramyxoviruses: Detection by Transmission Electron Microscopy Techniques

Catroxo¹,

Martins²,

Petrella³

et al. 2012

Int. J. Morphol.

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SUMMARY:Diseases caused by avian paramyxovirus (APMV) occur in commercial, captive and wild birds worldwide, demonstrating the significant economic and ecological importance of these agents. Paramyxoviruses belong to the Paramyxoviridae family, Paramyxovirinae subfamily and Avulavirus genus. During the period 2000 to 2011, stool and small intestine samples of 1647 birds species were sent to the Laboratory of Electron Microscopy, Biological Institute of São Paulo, Brazil, for diagnosis of viral agents. The samples were processed by negative staining (rapid preparation) and resin embedding techniques. Under the transmission electron microscope by negative staining technique, in 294 (17.8%) samples of 1647 were visualized paramyxovirus particles pleomorphic, roughly spherical or filamentous, measuring 100 to 500 nm of diameter containing an envelope covered with spikes and characteristic helical herring-bone-like nucleocapsid measuring 15 to 20 nm in diameter. Ultrathin sections of the small intestine fragments revealed the presence of amorphous granular intracytoplasmic inclusions surrounded by membrane and containing viral nucleocapsid measuring 10-14 nm in diameter. Immature particles budding from cell membranes, pleomorphic, spherical and tubular particles containing viral nucleocapsid strands, and the complete particles measured up to 170 nm in diameter were seen in the cytoplasm. Intranuclear inclusions containing viral nucleocapsid were also visualized. Nuclei showed a marginalized chromatin.

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Section: Discussionsupporting

confidence: 90%

Avian Paramyxoviruses: Detection by Transmission Electron Microscopy Techniques

Catroxo¹,

Martins²,

Petrella³

et al. 2012

Int. J. Morphol.

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“…In contrast to our novel isolates, budding results in filamentous particles that later transform to a spherical shape. Moreover, neither orthomyxoviruses nor paramyxoviruses show the prominent circular RNP structure associated with the inner side of the plasma membrane at budding sites (Dubois-Dalcq et al 1984;King et al 2012 Granzow et al 1999;King et al 2012) that was not observed in cells infected with our isolates. In addition, while orthomyxovirus RNP strands were observed as 10-12 nm filamentous structures, paramyxovirus RNP was 18 nm thick and tubular, but distinctly thinner than the RNP strands in our isolates with a diameter of 28-32 nm.…”

Section: Discussionmentioning

confidence: 63%

“…After onset of cytopathogenic alterations, the monolayers were fixed and processed for electron microscopy as described by Granzow et al . (, ).…”

Section: Methodsmentioning

confidence: 99%

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Isolation and partial characterization of a novel virus from different carp species suffering gill necrosis – ultrastructure and morphogenesis

Granzow

Fichtner

Schütze

et al. 2013

Journal of Fish Diseases

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Two isolates of a novel enveloped RNA virus were obtained from carp and koi carp with gill necrosis. Both isolates behaved identically and could be propagated in different cyprinid cell lines forming large syncytia. The virus was sensitive to lipid solvents and neither exhibited haemadsorption/haemagglutination nor reverse transcriptase activity. Mature virus particles displayed a spherical shape with diameter of 100-350 nm after negative staining and 100-300 nm in ultrathin sections, covered by short projections of 8-10 nm in length. Maturation of virus progeny was shown to occur by budding and envelopment of the filamentous helical nucleocapsids at the cell surface. A detailed comparison of ultrastructure and morphogenesis of the novel virus isolates with selected arena-, ortho- and paramyxoviruses as possible candidates for evaluation of taxonomic classification yielded no consistency in all phenotypic features. Thus, on the basis of ultrastructure the novel virus isolates could not be assigned unequivocally to any established virus family.

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“…The specificity of the reaction in the immunofluorescence test is verified by the fact that foci of infected cells are clearly distinguishable from surrounding uninfected cells or from cells in uninfected control cups, respectively. As known for paramyxoviruses, antigen seems to be present predominantly in the cytoplasm, but can also appear in some of the nuclei, described as intranuclear inclusion bodies by Granzow et al (1999).…”

Section: Titre [Log2]mentioning

confidence: 98%

Evaluation of Newcastle disease virus immunoassays for waterfowl using a monoclonal antibody specific for the duck immunoglobulin light chain

et al. 2008

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In the present study a monoclonal antibody (mAb 14A3) was tested for its reactivity against serum immunoglobulin Y (IgY) of several waterfowl species, and subsequently for its applicability as anti-species antibody in common immunoassays. Western blot analyses demonstrated its broad cross-reactivity with the serum IgY light chain of different duck species: Muscovy duck (Cairina moschata), Mallard (Anas platyrhynchos), white-winged wood duck (Asarcornis scutulatus), common pintail (Dafila acuta). Reactivity was also evident with IgY of two swan species*mute swan (Cygnus olor) and black-necked swan (Sthenelides melanocoryphus)*and two goose species*domestic goose (Anser anser var. domestica) and redbreasted goose (Rufibrenta ruficollis). Applying the mAb for Newcastle disease virus (avian paramyxovirus serotype 1 [APMV-1]) test systems, its functionality within indirect immunoassays was evaluated. Using APMV-1-positive sera of domestic geese and Muscovy ducks, mAb 14A3 facilitated specific staining of APMV-1-infected cells in an immunofluorescence test. In addition, it proved to be functional in an indirect enzyme-linked immunosorbent assay (ELISA) and a western blot assay. Thus, the analysed mAb represents an attractive and versatile reagent that offers the opportunity to develop serological tests for waterfowl, allowing a high sample throughput using the ELISA technique or the fine analysis of humoral immune responses using the western blot.

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Intranuclear Inclusions in Cells Infected with Newcastle Disease Virus

Cited by 6 publications

References 22 publications

Avian Paramyxoviruses: Detection by Transmission Electron Microscopy Techniques

Avian Paramyxoviruses: Detection by Transmission Electron Microscopy Techniques

Isolation and partial characterization of a novel virus from different carp species suffering gill necrosis – ultrastructure and morphogenesis

Evaluation of Newcastle disease virus immunoassays for waterfowl using a monoclonal antibody specific for the duck immunoglobulin light chain

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