Intraoperative autologous blood transfusion: report of a technique.

Khan, Raheem M. A.; Bassett, H. F. M.

doi:10.1136/thx.30.4.447

Cited by 4 publications

(2 citation statements)

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“…Previously a few authors have cultured circulating venous blood during autologous transfusion and have reported it to be sterile. Khan & Bassett (1975) cultured blood directly from the collecting bottle and recovered skin saprophytes in most cases from a pericardial blood pool. The present report is the first to quantify the degree of contamination by bacterial counting.…”

Section: Discussionmentioning

confidence: 99%

Autologous blood collection in abdominal vascular surgery. Assessment of a low pressure blood salvage system with particular reference to the preservation of cellular elements, triglyceride, complement and bacterial content in the collected blood

Andrews

Bloor

2008

Clinical &Amp; Laboratory Haematology

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Summary. A simple, low pressure, blood scavenging system has been assessed in major abdominal vascular operations. The aim of this study was to assess the quality of blood obtained with this device and its suitability for reinfusion. Three areas of special interest, which have not been reported so far, are the fate of plasma complement, the load of fat aspirated after tissue dissection and the degree of bacterial contamination in the scavenged blood. The development of autologous blood scavenging systems is reviewed. The features which most affect the quality of scavenged blood are identified and their importance discussed by comparison with our experience. Key features of our system were: simplicity of the apparatus, controlled low‐pressure aspiration, the use of systemic heparin and the avoidance of mechanical pumps. The blood obtained was of excellent quality with good preservation of cellular elements, platelets and fibrinogen. Plasma total haemolytic complement, C3, and C4, fractions were preserved in normal, though slightly reduced, quantities. Lipid (triglyceride) content was minimally increased after filtration. Bacterial contamination was present in all cases, but at a very low level provided that aspiration was limited to the peritoneal cavity. This low‐level of contamination is not thought to be of great significance; its origin and importance are discussed

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Section: Discussionmentioning

confidence: 99%

Autologous blood collection in abdominal vascular surgery. Assessment of a low pressure blood salvage system with particular reference to the preservation of cellular elements, triglyceride, complement and bacterial content in the collected blood

Andrews

Bloor

2008

Clinical &Amp; Laboratory Haematology

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“…4 Various techniques have been employed for collection, filtration and transfusion of salvaged blood. 7 A successful case of retransfusion of salvaged blood using a simple technique is reported.…”

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A Case of Intraoperative Blood Salvage and Retransfusion

Sharma

Ali

2003

Ann Saudi Med

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SM Sharma, ZZ Ali, A Case of Intraoperative Blood Salvage and Retransfusion. 2003; 23(5): 294-295 Retransfusion of autologous blood collected fron body cavities may be a life-saving measure when compatible homologous blood is not available. It may also prove to be a blessing in disguise because it eliminates the possibility of transfusion hazards such as iso-immunization, mismatch, and transmission of diseases. Spread of HIV and AIDS through the use of whole blood and blood products lends further support to the use of autohemotransfusion. 1,9,10 Use of blood collected from the peritoneal cavity in patients suffering from a ruptured ectopic gestation or splenic rupture has been a fairly common practice in the past.2 Despite being the most readily available source of compatible blood this method has not gained wide clinical importance because of cumbersome technique and lack of adequate knowledge about changes occurring in the blood shed into body cavities before and during the procedure of collection and retransfusion.3 Use of blood obtained by intraoperative salvage may be complicated by hemolysis, disseminated intravascular coagulation, sepsis and air embolism. 4 Various techniques have been employed for collection, filtration and transfusion of salvaged blood.7 A successful case of retransfusion of salvaged blood using a simple technique is reported. Case ReportA 25-year-old female was admitted with complaints of hypogastric pain and vomiting of 4 days' duration. She was not constipated, but had a low-grade fever (37.6°C). On admission she had a haemoglobin of 10 g/dL with a haematocrit of 30%. She had mild tachycardia (heart rate 110/min) with an arterial pressure of 98/70 mm Hg. Next morning (about 20 hours later) she was found to be grossly anaemic with the haemoglobin level having dropped to 5.0 g/dL. A peritoneal tap was haemorrhagic. No compatible blood was available in the hospital as the patient was found to belong to the B Rh-negative blood group.In view of intra-abdominal bleeding an emergency laparotomy was performed. Before incising the peritoneum arrangements had been made to collect the blood in the peritoneal cavity into sterile bottles containing acid-citratedextrose solution through a sterile 1.8 meter long rubber tube of 5 mm internal diameter. Approximately 2 litres of blood could be collected in about 7 minutes using a high vacuum suction apparatus. This blood was sieved aseptically through two layers of sterile gauze moistened with isotonic saline (0.89% NaCl solution) into four 500 mL capacity autoclaved glass bottles. On opening the abdomen a ruptured ectopic gestation was found. It was managed by the gynaecologist.Anaesthetic management consisted of inducing anaesthesia with diazepam (5 mg) and ketamine (75 mg), endotracheal intubation facilitated by use of suxamethonium (50 mg). Anaesthesia was maintained using pancuronium (6 mg), morphine (6mg), nitrous oxide and oxygen (50:50). Intra-operatively, crystalloids (1.5 L) including 1000 mL of lactated Ringer's solution, and 500 mL of i...

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S

Elks¹,

Ganellin²

1990

Dictionary of Drugs

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Intraoperative autologous blood transfusion: report of a technique.

Cited by 4 publications

References 13 publications

Autologous blood collection in abdominal vascular surgery. Assessment of a low pressure blood salvage system with particular reference to the preservation of cellular elements, triglyceride, complement and bacterial content in the collected blood

Autologous blood collection in abdominal vascular surgery. Assessment of a low pressure blood salvage system with particular reference to the preservation of cellular elements, triglyceride, complement and bacterial content in the collected blood

A Case of Intraoperative Blood Salvage and Retransfusion

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