Intraoperative bioprinting of human adipose-derived stem cells and extra-cellular matrix induces hair follicle-like downgrowths and adipose tissue formation during full-thickness craniomaxillofacial skin reconstruction

Kang, Youngnam; Yeo, Miji; Derman, Irem Deniz; Ravnic, Dino J.; Singh, Yogendra Pratap; Alioglu, Mecit Altan; Wu, Yang; Makkar, Jasson; Driskell, Ryan R.; Ozbolat, Ibrahim T.

doi:10.1016/j.bioactmat.2023.10.034

Cited by 5 publications

(1 citation statement)

References 39 publications

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“…Numerous studies have demonstrated that during in vitro cultivation, ADSCs could secret a multitude of bioactive cytokines and growth factors, including transforming growth factor-β, hepatocyte growth factor (HGF) and vascular endothelial growth factor (VEGF) ( Qin et al, 2023 ; Wang et al, 2023 ; Wu et al, 2023 ). These mediators are instrumental in fostering cell proliferation, cellular migration, angiogenesis regulation, immune response modulation, and inflammation response regulation, assuming an indispensable role in the wound repair process ( Song Y. et al, 2023 ; Wu et al, 2023 ; Kang et al, 2024 ). Following decellularization, these cytokines and growth factors partially remained within the dECM, which is closer to the natural ECM, thus enabling the dECM to exert a potential substantial impact in tissue regeneration.…”

Section: Introductionmentioning

confidence: 99%

Adipose-derived stem cells derived decellularized extracellular matrix enabled skin regeneration and remodeling

Zhang,

Xiang,

Yang

et al. 2024

Front. Bioeng. Biotechnol.

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The tissues or organs derived decellularized extracellular matrix carry immunogenicity and the risk of pathogen transmission, resulting in limited therapeutic effects. The cell derived dECM cultured in vitro can address these potential risks, but its impact on wound remodeling is still unclear. This study aimed to explore the role of decellularized extracellular matrix (dECM) extracted from adipose derived stem cells (ADSCs) in skin regeneration.Methods: ADSCs were extracted from human adipose tissue. Then we cultivated adipose-derived stem cell cells and decellularized ADSC-dECM for freeze-drying. Western blot (WB), enzyme-linked immunosorbent assay (ELISA) and mass spectrometry (MS) were conducted to analyzed the main protein components in ADSC-dECM. The cell counting assay (CCK-8) and scratch assay were used to explore the effects of different concentrations of ADSC-dECM on the proliferation and migration of human keratinocytes cells (HaCaT), human umbilical vein endothelia cells (HUVEC) and human fibroblasts (HFB), respectively. Moreover, we designed a novel ADSC-dECM-CMC patch which used carboxymethylcellulose (CMC) to load with ADSC-dECM; and we further investigated its effect on a mouse full thickness skin wound model.Results: ADSC-dECM was obtained after decellularization of in vitro cultured human ADSCs. Western blot, ELISA and mass spectrometry results showed that ADSC-dECM contained various bioactive molecules, including collagen, elastin, laminin, and various growth factors. CCK-8 and scratch assay showed that ADSC-dECM treatment could significantly promote the proliferation and migration of HaCaT, human umbilical vein endothelia cells, and human fibroblasts, respectively. To evaluate the therapeutic effect on wound healing in vivo, we developed a novel ADSC-dECM-CMC patch and transplanted it into a mouse full-thickness skin wound model. And we found that ADSC-dECM-CMC patch treatment significantly accelerated the wound closure with time. Further histology and immunohistochemistry indicated that ADSC-dECM-CMC patch could promote tissue regeneration, as confirmed via enhanced angiogenesis and high cell proliferative activity.Conclusion: In this study, we developed a novel ADSC-dECM-CMC patch containing multiple bioactive molecules and exhibiting good biocompatibility for skin reconstruction and regeneration. This patch provides a new approach for the use of adipose stem cells in skin tissue engineering.

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Section: Introductionmentioning

confidence: 99%

Adipose-derived stem cells derived decellularized extracellular matrix enabled skin regeneration and remodeling

Zhang,

Xiang,

Yang

et al. 2024

Front. Bioeng. Biotechnol.

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Extrusion/Inkjet Printing of Verteporfin-Loaded Bilayer Skin Substitutes for Wound Healing and Structure Reconstruction

Jiao,

Zhou,

Jiao

et al. 2024

J Bionic Eng

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Advancements in 3D skin bioprinting: processes, bioinks, applications and sensor integration

Derman,

Rivera,

Garriga Cerda

et al. 2024

Int. J. Extrem. Manuf.

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This comprehensive review explores the multifaceted landscape of skin bioprinting, revolutionizing dermatological research. The applications of skin bioprinting utilizing techniques like extrusion-, droplet-, laser- and light-based methods, with specialized bioinks for skin biofabrication have been critically reviewed along with the intricate aspects of bioprinting hair follicles, sweat glands, and achieving skin pigmentation. Challenges remain with the need for vascularization, safety concerns, and the integration of automated processes for effective clinical translation. The review further investigates the incorporation of biosensor technologies, emphasizing their role in monitoring and enhancing the wound healing process. While highlighting the remarkable progress in the field, critical limitations and concerns are critically examined to provide a balanced perspective. This synthesis aims to guide scientists, engineers, and healthcare providers, fostering a deeper understanding of the current state, challenges, and future directions in skin bioprinting for transformative applications in tissue engineering and regenerative medicine.

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Intraoperative bioprinting of human adipose-derived stem cells and extra-cellular matrix induces hair follicle-like downgrowths and adipose tissue formation during full-thickness craniomaxillofacial skin reconstruction

Cited by 5 publications

References 39 publications

Adipose-derived stem cells derived decellularized extracellular matrix enabled skin regeneration and remodeling

Adipose-derived stem cells derived decellularized extracellular matrix enabled skin regeneration and remodeling

Extrusion/Inkjet Printing of Verteporfin-Loaded Bilayer Skin Substitutes for Wound Healing and Structure Reconstruction

Advancements in 3D skin bioprinting: processes, bioinks, applications and sensor integration

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