Intraoperative imprint cytology versus histological diagnosis for the detection of sentinel lymph nodes in breast cancer treated with neoadjuvant chemotherapy

Bocanegra, Ronald Enrique Delgado; Millen, Eduardo Camargo; Nascimento, Cristina Moreira do; Bruno, Karine de Aguiar

doi:10.6061/clinics/2018/e363

Cited by 7 publications

(4 citation statements)

References 28 publications

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“…Majority (36.7%) of patients were of the age group 41–50 years with a mean age of 48.1 ± 10.6 years which was similar to the distribution of previous studies. [ 8 - 11 ] Most (90%) of the cases in the present study were invasive carcinoma of no special type (ductal) which is concordance to previous data. [ 8 - 12 ] There were six cases of SLN which were reported as negative for malignancy on TIC and turned out to be malignant on histopathological sections.…”

Section: Discussionsupporting

confidence: 92%

“…[ 8 - 11 ] Most (90%) of the cases in the present study were invasive carcinoma of no special type (ductal) which is concordance to previous data. [ 8 - 12 ] There were six cases of SLN which were reported as negative for malignancy on TIC and turned out to be malignant on histopathological sections. The possible reason behind this could be a low-grade tumor mimicking the background lymphocytes leading to a false-negative report.…”

Section: Discussionsupporting

confidence: 92%

“…In contrast, frozen section examination despite being reliable is a time-consuming and expensive technique. [ 8 ]…”

Section: Discussionmentioning

confidence: 99%

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Efficacy of intraoperative imprint cytology of sentinel lymph node in breast cancer

Yadav,

Ahuja,

Zaheer

et al. 2024

Cytojournal

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Objective: The most important determinant of patient outcome in cases of breast carcinoma is the regional lymph node status. Intraoperative assessment of sentinel lymph nodes (SLNs) allows the surgeon to perform axillary lymph node dissection in the same sitting if required. The commonly performed intraoperative methods for SLN evaluation are touch imprint cytology (TIC) and frozen section. The present study aimed to determine the sensitivity, specificity, and accuracy of TIC with histopathological diagnosis as gold standard. Material and Methods: The lymph nodes sent for intraoperative examination were bisected along the long axis and touched onto clean glass slides followed by Toluidine blue and rapid Papanicolaou staining. The imprints were reviewed and the interpretation was conveyed to the surgeon. Thereafter, the biopsy was fixed in 10% formalin followed by paraffin embedding with hematoxylin and eosin staining. The specificity, sensitivity, diagnostic accuracy, positive predictive value, and negative predictive value were evaluated with histopathological diagnosis as gold standard. Results: A total of 60 patients who underwent resection surgery were included in the study. Majority (36.7%) of patients were in the age group 41–50 years with a mean age of 48.1 ± 10.6 years. There were 54 cases (90%) and 6 cases (10%) of invasive carcinoma of no special type (ductal) and lobular carcinoma, respectively. According to modified Bloom–Richardson scoring, the cases were categorized as Grade 1–6 cases (10%), Grade 2–36 (60%), and Grade 3–18 (30%). The sensitivity and specificity of TIC were 87.5% and 100%, respectively. The diagnostic accuracy of TIC in the diagnosis of metastasis in SLN was 90%. Conclusion: TIC is an easy-to-perform, cost-effective, rapid, and accurate technique for axillary lymph node evaluation, which also overcomes the need for a cryostat.

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Section: Discussionsupporting

confidence: 92%

Section: Discussionsupporting

confidence: 92%

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Efficacy of intraoperative imprint cytology of sentinel lymph node in breast cancer

Yadav,

Ahuja,

Zaheer

et al. 2024

Cytojournal

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“…This histopathological evaluation was considered as the gold standard in the assessment of lymph node metastasis. [36][37][38]…”

Section: Histopathologymentioning

confidence: 99%

<p>Proteomics of Sentinel Lymph Nodes in Early Breast Cancer for Identification of Thymidylate Synthase as a Potential Biomarker to Flag Metastasis: A Preliminary Study</p>

Pathania

Khan

Kumar

et al. 2020

CMAR

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Introduction: Breast cancer is the second most common cancer in women across the world. Some of the patients who present in the early stage of disease are affected by metastasis to the axillary group of lymph nodes. The first among this group that is affected is called as sentinel lymph node, and its diagnosis is crucial for the staging of cancer thereby dictating the type of surgical therapy. Therefore, the sentinel lymph node status provides the most relevant information to the surgeon and patient prognosis. The expanded utilization of breast conservation surgery has declined the morbidity associated with mastectomy and axillary lymph node surgery. Recent interest is, therefore, centered on techniques that allow accurate assessment of the sentinel lymph node metastasis. A current procedure such as sentinel lymph node biopsy (SLNB) that is used to assess axillary lymph node metastasis is neither specific nor sensitive, and besides, it is time-consuming. Objective: To compare the protein profiles between metastatic and non-metastatic lymph nodes to identify a biomarker that can flag lymph node metastasis. Materials and Methods: Women with early breast cancer were screened using mammography imaging and recruited to the study. Surgical resection was done to remove the breast tissue, and sentinel lymph node was identified using fluorescein and methylene blue tracer. Lymph node was sliced, and one set was sent for histopathology, which was considered the gold standard to assess the metastatic status of the lymph node. One set of slices was taken for proteomic experiments. Proteins were labelled with fluorescent cyanine tags and were subjected to difference gel electrophoresis experiment. Differentially expressed spots that had at least a twofold relative ratio and consistent pattern across three sets of biological replicate experiments were marked. Gel spots were trypsin digested and identified on mass spectrometry machine. Validation study was done by Western blot experiment on the same set of samples. Results: Thymidylate synthase has a twofold higher expression in the metastatic sentinel lymph nodes as compared to non-metastatic lymph nodes in early breast cancer patients. Conclusion: Differential in gel expression proteomics is an ideal platform for the identification of potential protein biomarker candidates that can differentiate metastatic from nonmetastatic lymph nodes in early breast cancer. The identification of thymidylate synthase offers a scope to develop an on-table diagnostic kit to assess the status of sentinel lymph nodes during mastectomy procedure to guide surgical management of axillary lymph nodes in early breast cancer.

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Intraoperative Touch Imprint Cytology in Breast Cancer Patients After Neoadjuvant Chemotherapy

Hadalin¹,

Pislar²,

Borštnar³

et al. 2022

Clinical Breast Cancer

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Intraoperative imprint cytology versus histological diagnosis for the detection of sentinel lymph nodes in breast cancer treated with neoadjuvant chemotherapy

Cited by 7 publications

References 28 publications

Efficacy of intraoperative imprint cytology of sentinel lymph node in breast cancer

Efficacy of intraoperative imprint cytology of sentinel lymph node in breast cancer

<p>Proteomics of Sentinel Lymph Nodes in Early Breast Cancer for Identification of Thymidylate Synthase as a Potential Biomarker to Flag Metastasis: A Preliminary Study</p>

Intraoperative Touch Imprint Cytology in Breast Cancer Patients After Neoadjuvant Chemotherapy

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