Intraovarian influence of bovine corpus luteum on oocyte morphometry and developmental competence, embryo production and cryotolerance

Garzon, Daniel Ernesto Argudo; Tenemaza, M.; Merchán, S. L.; Balvoa, José A.; Méndez, M. S.; Soria, M. E.; Galarza, Luis; Guanga, Luis Eduardo Ayala; Hernández–Fonseca, Hugo; Perea, Mariana S.; Perea, Fernando

doi:10.1016/j.theriogenology.2020.05.044

Cited by 11 publications

(6 citation statements)

References 73 publications

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“…Supporting the previous finding, substances produced by luteal tissue, likely Progesterone, seem to have adverse effects on oocyte competence, because maturation rate was lower when the oocytes were collected during the luteal stage or from ovaries of pregnant cats than in the follicular or inactive stage [18]. However, in ruminants, the CL had favorable effects on oocyte competence and embryonic development [29,30].…”

Section: Resultssupporting

confidence: 63%

Effect of estrous cycle stage on oocyte in vitro maturation of domestic cats reared under tropical conditions

Monasterio–Alemán,

Monasterio–Oquendo,

Zambrano–Vivas

et al. 2023

RC FCV-LUZ

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Oocyte maturation is a critical step for in vitro embryo production. In female cats, findings on the influence of the estrous cycle stage on oocyte quality and maturation are contradictory. Little is known about this phenomenon in female cats in the tropics. This study aimed to assess the effect of the estrous cycle stage on oocyte quality and subsequent capacity to complete nuclear maturation in cats in a tropical environment. Ovaries from 18 sexually matured cats were collected during ovariohysterectomy. Cumulus–oocyte complexes (COCs) were released from follicles by slicing and fragmentation of the ovarian cortex. According to morphological characteristics, COCs were classified into grades I–II (suitable) and III–IV (no suitable). Only suitable COCs from each cat were cultured for in vitro maturation. Nuclear oocyte maturation was assessed by the presence of a telophase I or metaphase II plate with extrusion of the first polar corpuscle. A significantly greater number of oocytes per ovary were collected from queens in inactive than in follicular or luteal phase. Proportions of suitable COCs were similar among groups. Rate of oocyte maturation did not differ among stages of the estrous cycle, nor did the proportion of non–matured or degenerated oocytes. The age of the queens did not affect the percentage of oocyte maturation. In conclusion, the quality and rate of oocytes maturation were similar in the three stages of estrous cycle examined.

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Section: Resultssupporting

confidence: 63%

Effect of estrous cycle stage on oocyte in vitro maturation of domestic cats reared under tropical conditions

Monasterio–Alemán,

Monasterio–Oquendo,

Zambrano–Vivas

et al. 2023

RC FCV-LUZ

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“…In alpacas, cytoplasmic maturation studies have not yet been reported, being the first in this study. However, in cattle, rates of 80.3% [36] and 77.75% have been obtained by aspirating follicles from 4 to 8 mm using OPU [21]. Furthermore, in this same study, no significant differences were found in animals with and without super ovulation treatment.…”

Section: Discussionmentioning

confidence: 61%

Comparison of Two In vitro Maturation Media in Their Efficiency for Production of Competent Alpaca Oocytes

DDH¹,

FWG²,

MGP³

et al. 2022

J Anim Sci Res

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Oocyte maturation is the most critical process in in vitro culture, since the oocyte acquires competence for future processes, which involve the resumption of meiosis, epigenetic regulation, polyspermy block, activation of the zygote, exchange of protamines to histones, cleavage, cell polarization, embryonic stem cell proliferation, and implantation. Therefore, the objective of this study was to evaluate the efficiency of two different in vitro maturation media in the production of competent alpaca oocytes matured in vitro. The competency evaluated by morphometry and cytoplasmic and nuclear staining techniques. Alpaca ovaries (n=44) were sourced from slaughterhouse, transported to laboratory in thermoses with saline solution at 33-36ºC and subjected to follicular aspiration and/or dissection. A total of 212 oocytes were obtained. The oocytes were selected, randomized and distributed in one of the two experimental groups: Maturation medium 1 (MM1) and Maturation medium 2 (MM2) with three replicates per experiment. In vitro maturation was carried out in a portable incubator for 40h under 5% CO2 conditions (produced by effervescent granules) and 38.5ºC (by thermostatic bath). Nuclear maturation was assessed using acetate-orcein staining, cytoplasmic maturation using Brilliant Cresyl Blue (BCB) staining and morphometry using ImageJ Software. Nuclear maturation was classified according to the meiotic stage. Cytoplasmic maturation was classified by the presence (BCB+) or absence (BCB-) of BCB staining. Morphometry evaluated the measurements of the diameter and thickness of the zona pellucida and perivitelline space of the oocyte. Nuclear maturation rate was evaluated in Metaphase, observing no significant differences (P=0.088) between MM1 (46.08 ± 5.90 %) and MM2 (54.65 ± 3.00%) in this parameter. The cytoplasmic maturation rate of MM1 (67.78 ± 4.50%) was significantly lower compared to MM2 (77.61 ± 3.58) % (P=0.014). For the diameter of the oocyte, MM1 (133.42 ± 14.56 μm) and MM2 (136.14 ± 15.2 μm) were no different (P=0.397), same for the thickness of the zona pellucida, MM1 (16.84 ± 3.96 μm) and MM2 (16.73 ± 4.98 μm) were no different (P=0.919). For the perivitelline space area, MM1 (11018.91 ± 2465.40 μm2 ) and MM2 (8686.18 ± 3016.88 μm2 ) were significantly different (P=0.0005). In conclusion, the present study shows that the MM2 medium results are better in cytoplasmic maturation and the area of the perivitelline space. The other parameters (nuclear maturation, diameter of the oocyte and thickness of the zona pellucida) were no different between the two maturation media evaluated.

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“…Solo se observaron diferencias significativas entre los ovarios (p<0,05) en el porcentaje de CCOs de TFII y calidad CIII, aunque son estructuras que no se tienen en cuenta en procesos in vitro, respaldan una mayor actividad de crecimiento folicular en el ovario derecho. Asimismo, al comparar los porcentajes de CI y CII para TFI y TFII, se observó un mayor número CCOs de buena calidad en el ovario derecho, lo cual, según Ginther y Gómez-León (2020), puede explicar la mayor frecuencia de ovulaciones observadas en él, debido a su mayor irrigación o a la presencia de mayor número de cuerpos lúteos, como se observó en esta investigación y en el estudio de Argudo et al (2020). Estas características en los ovarios conllevan a una mejor competencia de los folículos en el proceso de crecimiento, sin embargo, se necesitan más estudios para confirmarlo.…”

Section: Discussionunclassified

“…En estudios se encontraron que los oocitos BCB-produjeron tasas más bajas de blastocistos al día ocho de cultivo in vitro, en comparación con los oocitos BCB+ (Torner et al, 2008), similar a lo reportado por Opiela et al (2008) y por Lamas-Toranzo et al (2018). Con relación a este tipo de oocitos evaluados, Rodríguez et al (2003) indicaron que tienen una mayor expresión del factor promotor de la maduración y actividad mitocondrial, otros factores que inciden en la competencia oocitaria, como una mayor proporción de oocitos BCB+ en función de la presencia de un cuerpo lúteo y asimismo una mayor tasa de producción de blastocistos (Argudo et al, 2020).…”

Section: Discussionunclassified

Morfometría de ovarios, folículos y su relación con la calidad oocitaria en bovinos

et al. 2022

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Introducción. Estudios sobre características estructurales del ovario asociadas con calidad del complejo cúmulos-oocito (COCs) en bovinos, contribuyen con la definición de estrategias para mejorar la eficiencia reproductiva. Objetivo. Determinar el peso y tamaño de los ovarios, calidad de los folículos y COCs en hembras Cebú, para establecer diferencias entre el ovario derecho e izquierdo. Materiales y métodos. Entre marzo y julio del 2017, se obtuvieron 52 ovarios de veintiséis vacas Cebú. Los ovarios y folículos se pesaron y midieron. Los folículos se agruparon en tres categorías: a) <3 mm (TFI), b) 3-6 mm (TFII) y c) >6 mm (TFIII). La calidad de los folículos se determinó mediante la técnica de irrigación-traslucidez (TIT) y la de COCs por las características del citoplasma y células del cúmulo como buena (CI), regular (CII) y mala (CIII), y mediante la prueba azul brillante de cresilo se categorizaron como BCB+ y BCB-. Resultados. La longitud, ancho y peso de los ovarios fueron 2,7 ± 0,6 cm, 1,8 ± 0,5 cm y 7,0 ± 3 g, respectivamente, con diferencias para longitud y peso entre ovario derecho e izquierdo (p<0,01). La mayor cantidad de folículos se obtuvieron en TFI (p<0,05). El 88,56 % de los folículos evaluados fueron de buena calidad. El 52 % y 44,2 % de COCs fueron clasificados como CI y BCB+, respectivamente. Cuando se consideró el tamaño del folículo y la calidad del COCs, se evidenció que en el grupo TFI el 22 % fueron CI, el 34 % CII y el 44 % CIII, y el TFII el 31 % fue CI, el 21 % CII y el 48 % CIII. No se observan diferencias entre ovario izquierdo y derecho. Conclusión. El ovario derecho presentó un mayor tamaño y peso, mayor número de estructuras foliculares y mayor número de CCOs de buena calidad.

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Intraovarian influence of bovine corpus luteum on oocyte morphometry and developmental competence, embryo production and cryotolerance

Cited by 11 publications

References 73 publications

Effect of estrous cycle stage on oocyte in vitro maturation of domestic cats reared under tropical conditions

Effect of estrous cycle stage on oocyte in vitro maturation of domestic cats reared under tropical conditions

Comparison of Two In vitro Maturation Media in Their Efficiency for Production of Competent Alpaca Oocytes

Morfometría de ovarios, folículos y su relación con la calidad oocitaria en bovinos

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