Intrapartum Test for Detection of Group B Streptococcus Colonization During Labor

Picchiassi, Elena; Coata, Giuliana; Babucci, Giulia; Giardina, Irene; Summa, Valentina; Tarquini, Federica; Centra, Michela; Bini, Vittorio; Cappuccini, Benito; Renzo, Gian Carlo Di

doi:10.1097/01.ogx.0000557707.43337.84

Cited by 2 publications

(3 citation statements)

References 0 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…While molecular assays have been shown to be rapid, sensitive and specific, they are generally associated with higher material costs (Poncelet‐Jasserand et al ). However, several studies suggest that the reduction in disease associated with appropriate detection and treatment in addition to reduction of unnecessary IAP may balance this cost (El Helali et al ; Picchiassi et al ). Another limitation of molecular methods is that the GBS strains are not cultured and are thus not available for antimicrobial susceptibility testing, which is crucial for patients with penicillin allergies.…”

Section: Introductionmentioning

confidence: 99%

Detection of group B Streptococcus during antenatal screening in Western Australia: a comparison of culture and molecular methods

Furfaro

Chang

Payne

2019

J of Applied Microbiology

View full text Add to dashboard Cite

Aim Global screening strategies for Group B Streptococcus (GBS) include risk‐ or culture‐based methods to guide intrapartum prophylaxis. In Western Australia (WA), antenatal culture‐based screening is routine; however, numerous culture methods exist, in addition to molecular methods. We aimed to assess the comparability of research and diagnostic screening approaches. Methods and Results Vaginal and rectal swabs were self‐collected by pregnant women (n = 531) from King Edward Memorial Hospital, WA, in parallel to routine screening (35–37 weeks of gestation). Research methods involved culture (Strep B Carrot Broth™ and StrepB CHROMagar™) and molecular methods (real‐time PCR) and were compared to routine diagnostic screening (Lim Broth and Granada agar). Overall, GBS detection was comparable between research and diagnostic approaches (3–5% discrepancy, kappa = 0·76). Specificity/sensitivity of Carrot Broth™ was 100%/89%, while that of CHROMagar™ was 73%/100%, respectively. Direct PCR was unable to detect GBS in ~18% of specimens which were culture positive; however, it exhibited 100% specificity. Conclusions This clinical evaluation of GBS screening methods provides support for current practice. Significance and Impact of the Study Although CHROM was highly sensitive, further testing is recommended due to a high false‐positive rate. Molecular assays are useful for rapid detection; however, low‐titre samples may require additional enrichment prior to molecular analysis to improve sensitivity.

show abstract

Section: Introductionmentioning

confidence: 99%

Detection of group B Streptococcus during antenatal screening in Western Australia: a comparison of culture and molecular methods

Furfaro

Chang

Payne

2019

J of Applied Microbiology

View full text Add to dashboard Cite

show abstract

“…In a previous evaluation [17] of the performance of the Xpert GBS rapid test when performed intrapartum, 13.4% of performed tests failed to yield a valid result on the first attempt (7.3% erroneous, 4.4% invalid, and 1.6% yielded no result). Another study [22] reported an invalid result rate of 13.6%, while Mueller [21] reported 13.4% after a 2-hour training session on thermocycler operation.…”

Section: Discussionmentioning

confidence: 93%

“…In a study [16] carried out among women in the Southeast region of Brazil, the prevalence of GBS by the culture method was around 18%, although only vaginal samples were collected. In an Italian sample of pregnant women [17], about 20% were GBS-positive with the culture method. The prevalence of GBS by culture in the present study (14.3%) was similar to the results of previous studies [4,14,16].…”

Section: Discussionmentioning

confidence: 96%

Group B Streptococcus detection in pregnant women: comparison of real-time PCR assay, culture, and the Xpert GBS rapid test

Vieira¹,

Perez²,

Machado³

et al. 2019

Preprint

View full text Add to dashboard Cite

Background Group B Streptococcus (GBS) is one of the most important causative agents of neonatal sepsis. As administration of prophylactic antibiotics during labor can prevent GBS infection, routine screening for this bacterium in prenatal care before the onset of labor is recommended. However, many women present in labor without having undergone such testing during antenatal care, and the turnaround time of detection methods is insufficient for results to be obtained before delivery. Methods Vaginal and anorectal specimens were collected from 270 pregnant women. Each sample was tested by Xpert GBS, PCR, and culture for GBS detection. Results The overall prevalence of maternal GBS colonization was 30.7% according to Xpert GBS, 51.1% according to PCR, and 14.3% according to cultures. Considering the PCR method as the reference, the Xpert GBS had a sensitivity of 53% and specificity of 93%. Positive Xpert GBS results were associated with marital status (married or cohabitating) and with prematurity as a cause of neonatal hospitalization. Positive cultures were associated with ischemic–hypoxic encephalopathy requiring therapeutic hypothermia. Conclusions Combined enrichment/PCR and the Xpert GBS rapid test found a high prevalence of GBS colonization. The Xpert GBS technique gives faster results and could be useful for evaluating mothers who present without antenatal GBS screening results and are at risk of preterm labor, thus allowing institution of prophylactic antibiotic therapy. Trial Registration: The study was approved by the institutional Research Ethics Committee (CAAE: 59688316.0.0000.5327)

show abstract

Intrapartum Test for Detection of Group B Streptococcus Colonization During Labor

Cited by 2 publications

References 0 publications

Detection of group B Streptococcus during antenatal screening in Western Australia: a comparison of culture and molecular methods

Detection of group B Streptococcus during antenatal screening in Western Australia: a comparison of culture and molecular methods

Group B Streptococcus detection in pregnant women: comparison of real-time PCR assay, culture, and the Xpert GBS rapid test

Contact Info

Product

Resources

About