Intrapericardial Neoplasia in Dogs

Abstract

Abstract.A retrospective study was conducted from 1,919 necropsies of dogs, and 52 cases were selected on the basis of primary or metastatic neoplasia in the pericardium, the heart base, or the myocardium. Primary intrapericardial neoplasms were more frequent than intrapericardial metastases. Hemangiosarcomas were the most frequent primary neoplasms, followed by chemodectomas. Lymphomas were the most frequent metastatic neoplasms, followed by fibrosarcomas and rhabdomyosarcomas. Other neoplasms, either primary or metastatic, occurred rarely.Neoplasms affecting the pericardium, the heart base or the myocardium are infrequent in the dog, representing only a small proportion of necropsy cases. 6,27 Hemangiosarcomas 4,13,27 and chemodectomas 2,4,27,28 are the most frequently observed. Other mesenchymal and epithelial tumors have been sporadically reported. 1,4,7,8,11,12,14,15,17,18,20,23,[25][26][27] Ratios of primary intrapericardial versus metastatic neoplasms and of metastatic mesenchymatous versus epithelial neoplasms are unknown.A 6-year retrospective study was done to determine the incidence of primary or metastatic intrapericardial neoplasia in the dog. The relative frequency of primary versus metastatic and metastatic mesenchymatous versus epithelial neoplasms affecting the pericardium, the heart base, and the pericardium were also investigated. Materials and methodsSelection of cases. Necropsies were performed on 1,919 dogs over a 6-yr period (January 1990 to December 1995 at the Faculty of Veterinary Medicine, University of Montreal. Neoplasms were diagnosed in 184 cases, and 52 of these were selected for study based on their intrapericardial localization.Light microscopy. Tissues were fixed in buffered 10% formalin, embedded in paraffin, sectioned at 5 m, and stained with hematoxylin, phloxin, and saffron (HPS). Giemsa, periodic acid-Schiff, phosphotungstic acid-hematoxylin, Gridley's reticulin and/or Grimelius stains were used on selected sections.Immunoperoxidase. Monoclonal antibodies a were used to detect the presence of desmin, vimentin, alpha-smooth muscle actin (SMA), myoglobin, and cytokeratin and polyclonal antibodies were used for calcitonin, thyroglobulin, muramidase, alpha-1 antitrypsin, kappa-lambda light chains, a and factor VIII-related antigen b on selected neoplasms to confirm the diagnosis. Sections were cut at 5 m and mounted on Received for publication May 14, 1997.slides precoated with poly-D-lysine. Sections were stained by the avidin-biotin complex method using standard reagents. c Sections were deparaffinized in successive xylene immersions, dehydrated through graded ethanols, and rehydrated in distilled water. Endogenous peroxidase activity was blocked with 3% hydrogen peroxide diluted in methanol. Sections were incubated overnight at 4 C with appropriate primary antibody in a humidified chamber, followed by incubation at room temperature in a humidified chamber with appropriate biotinylated antibodies. Sections were then covered with freshly prepared avidin-biotin-peroxidase solu...

Prevalência e aspectos anatomopatológicos das neoplasias primárias do coração, de tecidos da base do coração e metastáticas, em cães do Sul de Minas Gerais (1994-2009)

“…IHQ pelo método estreptavidina-biotina-peroxidase, barra 50μm. diagnosticado como histiocitose maligna sistêmica (Fig.11) foi baseado nos aspectos histopatológicos e na forte marcação imuno-histoquímica para CD18 (Fig.12) (Walter & Rudolph 1996, Girard et al 1999, Ware & Hopper 1999, Aupperle et al 2007. O hemangiossarcoma primário ocorreu em um Pastor Alemão, descrita como a raça mais afetada por esse tipo de tumor (Aronsohn 1985, Walter & Rudolph 1996.…”

“…A neoplasia metastática mais frequente foi o linfoma, com 29,16% (7/24); dados semelhantes são descritos por Girard et al (1999). Em alguns tumores classiϐicados anteriormente dentro do grupo de células redondas, somente com a marcação imuno-histoquímica foi possível o diagnóstico deϐinitivo.…”

“…Tumores primários do coração são raros em caninos, sendo o hemangiossarcoma o mais frequentemente relatado (Walter & Rudolph 1996, Girard et al 1999, Ware & Hopper 1999. Os tumores da base do coração, considerados neoplasias primárias dos tecidos extracardíacos, como o quimiodectoma (Kim et al 2005, Aupperle et al 2007, Headley et al 2009) e o carcinoma de tireoide ectópica (Constantino-Casas et al 1996, Walter & Rudolph 1996, Ware & Hopper 1999, ocorrem frequentemente em cães e podem afetar a função cardíaca, por comprimirem os vasos da base do coração e por inϐiltrarem no tecido cardíaco, como foi descrito por Almes et al (2008) em um carcinossarcoma de tireoide ectópica e por Cavalcanti et al (2006) em um quimiodectoma maligno.…”

“…O quimiodectoma é o tumor de maior ocorrência, depois do hemangiossarcoma cardíaco (Walter & Rudolph 1996, Ware & Hopper 1999. Também são descritos como tumores primários do coração em caninos o rabdomiossarcoma (Aupperle et al 2007), o rabdomioma (Girard et al 1999, Radi & Metz 2009, o ϐibrossarcoma (Madarame et al 2004, Speltz et al 2007, o neuroϐibrossarcoma (Aupperle et al 2007), os mesenquimomas malignos (tumores constituídos por vá-rios tipos de células mesenquimais) (Machida et al 2002), o schwannoma maligno (Wohlsein et al 2005), o condrossarcoma (Albers et al 1997, LaRock et al 1997, o osteossarcoma (Schelling & Moses 1994, Warman et al 2006, o mixoma (Symbas et al 1976), o mixoma valvular (Machida et al 2003, Akkoc et al 2007, o leiomiossarcoma (Fews et al 2008), o mesotelioma (Cobb & Brownlie 1992, Girard et al 1999, Brower et al 2006, o lipoma (Brambilla et al 2006, Ben-Amotz et al 2007) e o sarcoma mioϐibroblástico (Grieco et al 2008).…”

“…Num estudo retrospectivo de 83 cães com doença neoplásica em estágio terminal, em 11 foram diagnosticados tumores primários do coração e, em 24, metastáticos (Aupperle et al 2007). Vários tipos de neoplasia podem atingir o coração, entre eles carcinomas, linfomas, hemangiossarcoma (Aupperle et al 2007), ϐibrossarcoma, rabdomiossarcoma, melanoma, mastocitoma, feocromocitoma e histiocitose maligna disseminada (Girard et al 1999).…”