Intraperitoneal dye injection method for visualizing the functioning lymphatic vascular system in zebrafish and medaka

Saito, Erina; Isogai, Sumio; Deguchi, Tomonori; Ishida, Katsuaki; Nozaki, Taiji; Ishiyama, Eri; Wayama, Marina; Shimoda, Hiroshi

doi:10.1002/dvdy.143

Cited by 3 publications

(3 citation statements)

References 34 publications

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“…Since LyVs readily absorbs cinnabar dye, the intraperitoneal injection of this dye into experimental animals allows visualization of lymphatic vessels in whole embryos or whole organs of adult animals in standard histological and transmission electron microscopy sections. 78 Based on the expression of Prox1, Lyve1, VEGFR-3, podoplanin, and other molecules in LEC, after whole-body immunostaining with specific antibodies to these molecules, the whole-body immunostaining and clarified tissue samples observed by light sheet microscopy presented important information about lymphatic vessels in 3-dimensional form (Figure 2). This technique can distinguish initial lymphangiectasia or degeneration from precollector or collector vessel changes in caliber or density, which have different functional effects on tissue drainage capacity.…”

Section: Visualization Techniques For Lymphatic Vasculature Molecular...mentioning

confidence: 99%

Role of Lymphangiogenesis in Cardiac Repair and Regeneration

Xu,

Lu,

Chen

et al. 2023

Methodist DeBakey Cardiovascular Journal

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This article highlights the importance of the structure and function of cardiac lymphatics in cardiovascular diseases and the therapeutic potential of cardiac lymphangiogenesis. Specifically, we explore the innate lymphangiogenic response to damaged cardiac tissue or cardiac injury, derive key findings from regenerative models demonstrating how robust lymphangiogenic responses can be supported to improve cardiac function, and introduce an approach to imaging the structure and function of cardiac lymphatics.

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Section: Visualization Techniques For Lymphatic Vasculature Molecular...mentioning

confidence: 99%

Role of Lymphangiogenesis in Cardiac Repair and Regeneration

Xu,

Lu,

Chen

et al. 2023

Methodist DeBakey Cardiovascular Journal

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“…Such studies have shown that zebrafish possess a lymphatic system that shares key structural and functional features with its mammalian counterpart [47,48]. Examples of functional similarities include the ability of zebrafish lymphatic vessels to clear injected dyes from extracellular spaces [9,[47][48][49][50] and the presence of collecting vessels with valves for unidirectional lymph flow [51].…”

Section: Techniques and Models To Visualise Lymphatics During Embryonmentioning

confidence: 99%

Mechanisms and cell lineages in lymphatic vascular development

et al. 2021

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Lymphatic vessels have critical roles in both health and disease and their study is a rapidly evolving area of vascular biology. The consensus on how the first lymphatic vessels arise in the developing embryo has recently shifted. Originally, they were thought to solely derive by sprouting from veins. Since then, several studies have uncovered novel cellular mechanisms and a diversity of contributing cell lineages in the formation of organ lymphatic vasculature. Here, we review the key mechanisms and cell lineages contributing to lymphatic development, discuss the advantages and limitations of experimental techniques used for their study and highlight remaining knowledge gaps that require urgent attention. Emerging technologies should accelerate our understanding of how lymphatic vessels develop normally and how they contribute to disease.

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“…Overall, this caudal lymphatic network recapitulates the patterns described upon injection of red fluorescent beads to fli1-GFP transgenic lines (vascular and lymphatic endothelial cells) 38 or injection of cinnabar dye. 39 Interestingly, high resolution 3D imaging of the Tg (batf3MIN:eGFP) eGFP pattern in the tail of juveniles highlights the elusive "lymphatic heart" in vivo. These observations will therefore enable to conduct functional studies to unveil the functions of lymphatics in the caudal tissue during inflammation/regeneration processes modeled in zebrafish over development.…”

mentioning

confidence: 99%

New reporter zebrafish line unveils heterogeneity among lymphatic endothelial cells during development

Frétaud

Khoa

Houel

et al. 2021

Developmental Dynamics

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Background: In zebrafish, lymphatic endothelial cells (LECs) originate from multiple/several distinct progenitor populations and generate organ-specific lymphatic vasculatures. Cell fate and tissue specificities were determined using a combination of genetically engineered transgenic lines in which the promoter of a LEC-specific gene drives expression of a fluorescent reporter protein.Results: We established a novel zebrafish transgenic line expressing eGFP under the control of part of the zebrafish batf3 promoter (Basic Leucine Zipper ATF-Like Transcription Factor 3). Spatiotemporal examination of Tg(batf3MIN:eGFP) transgenic fish revealed a typical lymphatic expression pattern, which does not perfectly recapitulate the expression pattern of existing LEC transgenic lines. eGFP + cells constitute a heterogeneous endothelial cell population, which expressed LEC and/or blood endothelial cells (BEC) markers in different tissues.In addition, we characterize the renal eGFP + cell as a population of interest to study kidney diseases and regeneration. Conclusion:Our Tg(batf3MIN:eGFP) reporter zebrafish line provides a useful system to study LEC populations, of which heterogeneity depends on origin of progenitors, tissue environment and physiological conditions. We further developed a novel fish-adapted tissue clearing method, which allows deep imaging and 3D-visualization of vascular and lymphatic networks in the whole organism.

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Intraperitoneal dye injection method for visualizing the functioning lymphatic vascular system in zebrafish and medaka

Cited by 3 publications

References 34 publications

Role of Lymphangiogenesis in Cardiac Repair and Regeneration

Role of Lymphangiogenesis in Cardiac Repair and Regeneration

Mechanisms and cell lineages in lymphatic vascular development

New reporter zebrafish line unveils heterogeneity among lymphatic endothelial cells during development

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