Intraperitoneal Injection of Multiplacentas Pooled Cells Treatment on a Mouse Model with Aplastic Anemia

Abstract

Coinfusion of hematopoietic and mesenchymal stem cells is more effective than hematopoietic stem cell transplantation alone. It is necessary to explore a safe and routine mixed stem cell intraperitoneal transplantation method. Multiplacentas pooled cells were intraperitoneally injected into a radiation- and immunity-induced mouse aplastic anemia model with single time. Then, mouse survival time, peripheral blood hemoglobin count, bone marrow architecture, and donor cell engraftment were assessed. The recipient mouse exhibited donor cell engraftment in both bone marrow and peripheral blood. Survival time and peripheral blood hemoglobin count increased in placenta pooled cells treated mice, compared with model-only controls (P = 0.048 and P = 0.000, resp.). However, placentas pooled cells failed to cause a significant decrease in bone marrow pimelosis area (P = 0.357). Intraperitoneally transplanted multiplacentas pooled cells can survive and engraft into a host body through blood circulation, which can increase the life span of an aplastic anemia model mice, and delay but not abrogate the development of aplastic anemia. Furthermore, they appear to play a role in increasing peripheral blood hemoglobin level response for increasing the life span of aplastic anemia model mice.

Multiplacenta derived stem cell/cytokine treatment increases survival time in a mouse model with radiation-induced bone marrow damage

“…In addition, the number of stem cells on the blood smear far exceeded that in the bone marrow smear, and the peripheral blood mesenchymal stem cell count in the blood smear was much higher than that of the hematopoietic stem cells. These findings suggest that cytokines boost the survival of mesenchymal stem cells and engraft then into the host body through the blood circulation (Li et al 2016). Mesenchymal stem cells can promote the survival and engraftment of the hematopoietic stem cells in the peripheral blood.…”

“…Sca1 ? cells) were sorted from green fluorescent proteinexpressing transgenic inbred parturient C57BL/6J mice as reported previously (Li et al 2016;Meng et al 2003;Wang et al 2006).…”

“…So, when mice started to die, mice in the detection subgroup were initially bled for peripheral blood donor cell engraftment detection and hemoglobin measurement. Then, the mice were sacrificed to collect the left and right femurs for marrow donor cell engraftment and architecture examination, respectively, as reported previously (Li et al 2016). The peripheral blood and the bone marrow were diluted with the same multiple DMEM/F-12 to detect the donor cells.…”

“…In addition, immunological mechanisms may facilitate engraftment in donors receiving two unrelated umbilical cord blood units (David McKenna and Sheth 2011). Using a mouse model, we have ascertained that intraperitoneal transplantation of multiplacenta-pooled cells can delay the development of aplastic anemia (Li et al 2016). Considering that the placenta contains abundant cytokines as well as hematopoietic and mesenchymal stem cells, it is plausible that treatment with multiplacenta-derived stem cells/cytokines can improve hematopoietic recovery.…”

Increased apoptosis and peripheral blood mononuclear cell suppression of bone marrow mesenchymal stem cells in severe aplastic anemia

“…We speculate that the poor proliferative potential and increased apoptosis of the SAA MSCs may be associated with their poor support. In vivo , MSC infusion has greatly benefited hematopoietic reconstitution in SAA animals . Therefore, MSC insufficiency is possibly responsible for their functionally abnormal support in SAA BM.…”