Intraphagosomal pH in Alveolar Macrophages after Phagocytosis In Vivo and In Vitro of Fluorescein-Labeled Yeast Particles

Nilsen, Asbjørn Magne; Nyberg, Kristina; Camner, Per

doi:10.3109/01902148809115124

Cited by 29 publications

(19 citation statements)

References 17 publications

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“…range of 5 (45)(46)(47)(48)(49)(50)(51)(52)(53)(54)(55)(56)(57)(58)(59)(60), it is plausible to conclude that the intracellular mechanism of particle dissolution is controlled by these two parameters. However, at this moment we cannot exclude other mechanisms of IPD resulting from proteolytic enzymes, oxygen radicals, chelators, precipitators, etc., that are contained in the phagolysosomal plasma (20).…”

Section: Mechanisms Of Intracellular Particle Dissolutionmentioning

confidence: 99%

Intracellular Particle Dissolution in Alveolar Macrophages

Kreyling¹

1992

Environmental Health Perspectives

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Aerosol particles deposited in the lungs that are not reaolily soluble in the epithelial lining fluid will be phagocytized by alveolar macrophages (AM). Inside the phagolysomal vacuole, the constituents ofthe plam allow dissolution ofavaiety ofcompounds at a higher rate than dissolutionietracelular lung fluids. Cielator concentration and a pH value ofabout 5 were found to control intracellular partice dissolution (IPD). Hence, IPD is the initial step oftranslocation ofdissolved material to blood, which is an important lung clearance nism for particles retined long term. IPD rtes of uniform test particles determined in human, baboon, and canine AM cultures were imilar to initialtrnslocation rates determined in lung clearnce studies ofthe same species after inhalation ofthe same test particles. IPD rate in cultured AM proved to be a sensitive functional parameter ofAM, which was used to identify changes in the clearance mechanism oftrnslocation during different exposure conditions.

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Section: Mechanisms Of Intracellular Particle Dissolutionmentioning

confidence: 99%

Intracellular Particle Dissolution in Alveolar Macrophages

Kreyling¹

1992

Environmental Health Perspectives

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“…In the measurements using FYP, there was a decrease in pH during the first day after lavage, both in the in vitro and the in vivo procedure, whereas in measurements with FSP, the pH was unchanged during the same period (14,15). Table 3 presents phagolysosomal pH using FYP and FSP in macro- Table 2.…”

Section: Comparison Of Ph Obtained With Spectrometer and Microscopementioning

confidence: 99%

“…Figure 1 show standard curves for FYP and FSP obtained from fluorescense measurements ofthe particles suspended in series of Hank's balanced salt solution (BSS) with various pHs. (14) and Nyberg et al (15,16).…”

Section: Particlesmentioning

confidence: 99%

“…Table 3 presents phagolysosomal pH using FYP and FSP in macro- Table 2. Phagolysosomal pH in samples from three rabbits estimated from measurements on individual phagolysosomes in a fluorescence microscope and on populations of macrophages in a fluorescence spectrometer (16 the pH was unchanged during the same period (14,15). Table 3 presents phagolysosomal pH using FYP and FSP in macrophages cultured for 3 and 24 hr.…”

Section: Comparison Of Ph Obtained With Spectrometer and Microscopementioning

confidence: 99%

“…In cell populations, using a fluorescence spectrometer, they estimated lysosomal pH from the relationship between the ratio of the fluorescenceat wavelength 519 nm and excitation wavelengths 495 and 450 nm and pH. We used this principle for measurements of phagolysosomal pH in alveolar macrophages with fluoresceinlabeled yeastparticles (FYP) or fluorescein-labeled amorphous silicaparticles (FSP) as probes (14)(15)(16)(17). The presentpaper reviews our studies with these particles concerning methodological aspects and includes examination of the influence of particle number, size, and material on pH, comparisons ofpH obtained from the testparticles in vitro and in vivo, as well as after various times after the administration ofparticles.…”

Section: Introductionmentioning

confidence: 99%

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Phagolysosomal pH in alveolar macrophages.

Nyberg

Johansson

et al. 1992

Environ Health Perspect

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VW tdi phagolysm pH in veobr macrophags (AM) using fluorescein-labeledyead (FYP) amn silica particles (FSP) as probes. Fluorescence intensities from the ingested test particles were measured on populations of AM using fluorescence spectometry and on individual phagolysosomes using fluorescence microscopy. Measurements were performed on rabbit AM, which had been incubated with FYPorFSP (in vitot procedure). ealso insd FYPor FSP via the trachea into rabbit lungs and after 1 day, 1 week, 1 month, and 3 months lavaged thelungs and measured the pH in AM (in vivo procedure). Phagolysosomal pH was independent of the number and size of the fluorescent particles. Measurementsof uadons of AM with fluorescence spec y and of hd ual phagolysosomes with fluorescence microscopy gave similar average pH. For the FYP, pH decreased during the first day after lavage both in the in vit and the in vivo procedures. For the FSP, pH was unchaneddurngthe same period. After 1 daypH was sinilar for both particles. Electron microscopy showed a larger number of lysosomes in contact with phagosomes and a higher pementage ofvacuolated phagosomesforFYPthanforFSP. Inthein uwoprocedure, pH was unchanged at least upto 1 month, and this pH was lower than that in the in vitrv procedure. The difference was probably due to conditions at the time of phagocyosis Particles retained in the lung parenchyma were within AM, and their location within the AM appeared unchanged from 1 week up to 3 months.

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Effect of some additives on the biostability of a poly(etherurethane) elastomer

Zhao

Anderson

et al. 1991

J. Biomed. Mater. Res.

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Four materials based on a single poly-(etherurethane) (PEU) prepared from MDI and PTMEG but differing in additives were studied in the cage implant system. The two additives studied were Santowhite powder at the 1% level and Methacrol 2138F 5%. Methacrol 2138F appeared to be immiscible with the base PEU and was dispersed in discrete domains about 0.5-micron in size. The retrieved PEU specimens were also cleaned and examined in the optical and scanning electron microscopes, and the size and density of adherent foreign body giant cells (FBGCs) were measured at implantation times up to 10 weeks. Methacrol 2138F had no effect on the density, coverage or size distribution of adherent FBGCs, but leaching of Methacrol 2138F was considered to be responsible for extensive pitting of the PEU surface. On the other hand, Santowhite powder appeared to inhibit formation of FBGCs, and while surface cracking and flaking were observed as early as 3 weeks postimplantation on some PEUs, the Santowhite powder effectively inhibited surface cracking and flaking up to the longest implantation time studied.

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Intraphagosomal pH in Alveolar Macrophages after Phagocytosis In Vivo and In Vitro of Fluorescein-Labeled Yeast Particles

Cited by 29 publications

References 17 publications

Intracellular Particle Dissolution in Alveolar Macrophages

Intracellular Particle Dissolution in Alveolar Macrophages

Phagolysosomal pH in alveolar macrophages.

Effect of some additives on the biostability of a poly(etherurethane) elastomer

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