Intraplaque and Cellular Distribution of Dextran-Coated Iron Oxide Fluorescently Labeled Nanoparticles

Calcagno, Claudia; Fayad, Zahi A.

doi:10.1161/circimaging.117.006533

Cited by 6 publications

(3 citation statements)

References 20 publications

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“…Oxidized LDL and inflammation are involved in the pathology of atherosclerosis; targeting macrophages, oxidized low-density lipoprotein (oxLDL), and microvessels might be useful in attenuating atherosclerosis. Recently, magnetic resonance imaging (MRI) with iron oxide nanoparticles has been extensively probed as a tool to detect inflammation in high-risk plaques vulnerable to rupture [25]. Iron oxide nanoparticles contain an iron oxide core and based on the size, they can be used for intravascular versus tissue imaging.…”

Section: Therapeutic Role In Vascular Diseasesmentioning

confidence: 99%

“…These nanoparticles can be labeled with radioisotopes or fluorophores to increase their diagnostic properties. The detection of iron oxide nanoparticles phagocytized by inflammatory cells and reticuloendothelial system cells may indicate the inflammatory cells involved in the process of inflammation and atherosclerosis [25]. Ferumoxtran-10 are ultra-small superparamagnetic iron oxides (SPIO) approved for treating iron-deficient anemia in chronic kidney disease and their uptake has been demonstrated in the symptomatic and contralateral asymptomatic carotid artery [26] and coronary artery disease [27].…”

Section: Therapeutic Role In Vascular Diseasesmentioning

confidence: 99%

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Nanomaterial-Mediated Theranostics for Vascular Diseases

Agrawal

Nooti

Singh

et al. 2020

JNT

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Nanotechnology could offer a new complementary strategy for the treatment of vascular diseases including coronary, carotid, or peripheral arterial disease due to narrowing or blockage of the artery caused by atherosclerosis. These arterial diseases manifest correspondingly as angina and myocardial infarction, stroke, and intermittent claudication of leg muscles during exercise. The pathogenesis of atherosclerosis involves biological events at the cellular and molecular level, thus targeting these using nanomaterials precisely and effectively could result in a better outcome. Nanotechnology can mitigate the pathological events by enhancing the therapeutic efficacy of the therapeutic agent by delivering it at the point of a lesion in a controlled and efficacious manner. Further, combining therapeutics with imaging will enhance the theranostic ability in atherosclerosis. Additionally, nanoparticles can provide a range of delivery systems for genes, proteins, cells, and drugs, which individually or in combination can address various problems within the arteries. Imaging studies combined with nanoparticles helps in evaluating the disease progression as well as the response to the treatment because imaging and diagnostic agents can be delivered precisely to the targeted destinations via nanocarriers. This review focuses on the use of nanotechnology in theranostics of coronary artery and peripheral arterial disease.

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Section: Therapeutic Role In Vascular Diseasesmentioning

confidence: 99%

Section: Therapeutic Role In Vascular Diseasesmentioning

confidence: 99%

Nanomaterial-Mediated Theranostics for Vascular Diseases

Agrawal

Nooti

Singh

et al. 2020

JNT

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“…Early studies recognized VSOP accumulation in atherosclerotic lesion, 1 h after IV injection in rabbits [ 38 ], as opposed to 3 days required for accumulation of ferumoxytol in atherosclerotic lesions, in hyperlipidemic rabbits [ 39 ]. Recently, the potential of VSOP [ 38 , 40 – 43 ] and other IONP for atherosclerotic plaques characterization has been explored [ 44 , 45 ].…”

Section: Introductionmentioning

confidence: 99%

Synthesis of europium-doped VSOP, customized enhancer solution and improved microscopy fluorescence methodology for unambiguous histological detection

et al. 2017

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BackgroundIntrinsic iron in biological tissues frequently precludes unambiguous the identification of iron oxide nanoparticles when iron-based detection methods are used. Here we report the full methodology for synthesizing very small iron oxide nanoparticles (VSOP) doped with europium (Eu) in their iron oxide core (Eu-VSOP) and their unambiguous qualitative and quantitative detection by fluorescence.Methods and resultsThe resulting Eu-VSOP contained 0.7 to 2.7% Eu relative to iron, which was sufficient for fluorescent detection while not altering other important particle parameters such as size, surface charge, or relaxivity. A customized enhancer solution with high buffer capacity and nearly neutral pH was developed to provide an antenna system that allowed fluorescent detection of Eu-VSOP in cells and histologic tissue slices as well as in solutions even under acidic conditions as frequently obtained from dissolved organic material. This enhancer solution allowed detection of Eu-VSOP using a standard fluorescence spectrophotometer and a fluorescence microscope equipped with a custom filter set with an excitation wavelength (λex) of 338 nm and an emission wavelength (λem) of 616 nm.ConclusionThe fluorescent detection of Eu-doped very small iron oxide nanoparticles (Eu-VSOP) provides a straightforward tool to unambiguously characterize VSOP biodistribution and toxicology at tissue, and cellular levels, providing a sensitive analytical tool to detect Eu-doped IONP in dissolved organ tissue and biological fluids with fluorescence instruments.Electronic supplementary materialThe online version of this article (doi:10.1186/s12951-017-0301-6) contains supplementary material, which is available to authorized users.

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