Intrarenal signaling mediated by CCK plays a role in salt intake-induced natriuresis

Abstract

The natriuretic hormone CCK exhibits its gene transcripts in total kidney extracts. To test the possibility of CCK acting as an intrarenal mediator of sodium excretion, we examined mouse kidneys by ) an in situ hybridization technique for CCK mRNA in animals fed a normal- or a high-sodium diet;) immuno-electron microscopy for the CCK peptide, ) an in situ hybridization method and immunohistochemistry for the CCK-specific receptor CCKAR;) confocal image analysis of receptor-mediated Ca responses in isolated renal tubules; and ) metabolic cage experiments for the measurement of urinary sodium excretion in high-salt-fed mice either treated or untreated with the CCKAR antagonist lorglumide. Results showed the CCK gene to be expressed intensely in the inner medulla and moderately in the inner stripe of the outer medulla, with the expression in the latter being enhanced by high sodium intake. Immunoreactivity for the CCK peptide was localized to the rough endoplasmic reticulum of the medullary interstitial cells in corresponding renal regions, confirming it to be a secretory protein. Gene transcripts, protein products, and the functional activity for CCKAR were consistently localized to the late proximal tubule segments (S2 and S3) in the medullary rays, and the outer stripe of the outer medulla. Lorglumide significantly diminished natriuretic responses of mice to a dietary sodium load without altering the glomerular filtration rate. These findings suggest that the medullary interstitial cells respond to body fluid expansion by CCK release for feedback regulation of the late proximal tubular reabsorption.

Targeted single-cell RNA-seq identifies minority cell types of kidney distal nephron that regulate blood pressure and calcium balance

“…The analysis also identified some GPCRs without well-characterized kidney functions including cholecystokinin A receptor (Cckar) in PT. A recent study indicated that it might regulate proximal tubular reabsorption 40 . Additional analyses were provided in this study including the distribution of aquaporins ( Supplementary Fig.…”

Photodynamic Activation of the Cholecystokinin 1 Receptor with Tagged Genetically Encoded Protein Photosensitizers: Optimizing the Tagging Patterns

“…CCK1R is expressed in the cerebral cortex and subcortical structures (basal ganglia, hippocampus, thalamus and hypothalamus) (1)(2)(3)(4)(5)(6)(7), in the brain stem and spinal dorsal horn (1)(2)(3). In the periphery, CCK1R is localized in trigeminal ganglia (8), the vagus (9,10), myenteric plexus (11), stomach (12,13), pancreas (12,14,15), gallbladder (12,16), intestines (17,18), adrenal cortex (19), kidney tubules (20), spleen (21), white adipose tissue (22) and leukocytes (23). Central CCK1R regulates peptide neurotransmitter release (24,25), anxiolysis (26)(27)(28), appetite (29), neurogenesis (4,30), learning and memory (31).…”