1973
DOI: 10.1073/pnas.70.12.3371
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Intrasomatic Injection of Radioactive Precursors for Studying Transmitter Synthesis in Identified Neurons of Aplysia californica

Abstract: We introduced radioactive precursors directly into identified neurons of Aplysia californica. [3HJ-Choline and L-[3H]tryptophan were injected with pressure into nerve cell bodies to study synthesis of acetylcholine and serotonin. We confirmed the cholinergic nature of R2, LIO, and Lii, identified neurons of the abdominal ganglion. Cells in the LD cluster (which contains motor neurons to the heart and gill) also converted most of the injected choline into acetylcholine. Neurons in the RB cluster (which contains… Show more

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Cited by 124 publications
(117 citation statements)
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“…were dissected out, treated with 0.5-l .O% protease (type XIV, Sigma) in artificial seawater at 18°C for 30-90 min, and allowed to recover for 2-6 d at 18°C in artificial seawater supplemented with amino acids, vitamins, sugar, penicillin, and streptomycin (Eisenstadt et al, 1973). The pleural sensory neuron cluster is a relatively pure preparation containing only sensory neuron cell bodies and neuropil .…”
Section: Isolated Sensory Clusters Clusters Of Pleural Mechanosensormentioning
confidence: 99%
“…were dissected out, treated with 0.5-l .O% protease (type XIV, Sigma) in artificial seawater at 18°C for 30-90 min, and allowed to recover for 2-6 d at 18°C in artificial seawater supplemented with amino acids, vitamins, sugar, penicillin, and streptomycin (Eisenstadt et al, 1973). The pleural sensory neuron cluster is a relatively pure preparation containing only sensory neuron cell bodies and neuropil .…”
Section: Isolated Sensory Clusters Clusters Of Pleural Mechanosensormentioning
confidence: 99%
“…The central nervous system was removed intact and the R2 cell soma was pressure-injected with 10% HRP (Sigma Chemicals, type VI) in 0.1 N NaOH (Rayport et al, 1983). After injection, the nervous system was maintained in artificial seawater (Eisenstadt et al, 1973) for 48 hr at 15"C, fixed with 6% glutaraldehyde in S-collidine 'buffer; reacted with diaminobenzidine, dehydrated, and embedded in Permount (Bailey et al,198 1). Tissue was examined by Nomarski differential interference optics and by electron microscopy.…”
Section: Methodsmentioning
confidence: 99%
“…(Palovcik et al, 1982;Mackey and Carew, 1983;Kabotyanski et al, 2000). In the feeding system, serotonin is expressed in the MCC neurons (Eisenstadt et al, 1973;Weinreich et al, 1973), which mediate food-induced arousal of the feeding network Rosen et al, 1989). The MCCs do not, however, trigger locomotor activity or modulate the loco- motor network.…”
Section: Introductionmentioning
confidence: 99%